ID   CML22_ARATH             Reviewed;         229 AA.
AC   Q9LRN6;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Probable calcium-binding protein CML22;
DE   AltName: Full=Calmodulin-like protein 22;
GN   Name=CML22; OrderedLocusNames=At3g24110; ORFNames=MUJ8.1;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   DOI=10.1046/j.1469-8137.2003.00845.x;
RA   McCormack E., Braam J.;
RT   "Calmodulins and related potential calcium sensors of Arabidopsis.";
RL   New Phytol. 159:585-598(2003).
CC   -!- FUNCTION: Potential calcium sensor. {ECO:0000250}.
CC   -!- CAUTION: Although assigned as a calmodulin family member by Ref.3, it
CC       only contains EF-hand domains. {ECO:0000305}.
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DR   EMBL; AB028621; BAB01352.1; -; Genomic_DNA.
DR   EMBL; CP002686; ANM65247.1; -; Genomic_DNA.
DR   RefSeq; NP_189053.1; NM_113316.2.
DR   AlphaFoldDB; Q9LRN6; -.
DR   STRING; 3702.AT3G24110.1; -.
DR   iPTMnet; Q9LRN6; -.
DR   PaxDb; Q9LRN6; -.
DR   PRIDE; Q9LRN6; -.
DR   ProteomicsDB; 241077; -.
DR   EnsemblPlants; AT3G24110.2; AT3G24110.2; AT3G24110.
DR   GeneID; 821997; -.
DR   Gramene; AT3G24110.2; AT3G24110.2; AT3G24110.
DR   KEGG; ath:AT3G24110; -.
DR   Araport; AT3G24110; -.
DR   TAIR; locus:2093716; AT3G24110.
DR   eggNOG; KOG0027; Eukaryota.
DR   HOGENOM; CLU_069274_1_1_1; -.
DR   InParanoid; Q9LRN6; -.
DR   OMA; LSIIRWA; -.
DR   PhylomeDB; Q9LRN6; -.
DR   PRO; PR:Q9LRN6; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LRN6; baseline and differential.
DR   Genevisible; Q9LRN6; AT.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   CDD; cd00051; EFh; 1.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13499; EF-hand_7; 1.
DR   SMART; SM00054; EFh; 2.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 4.
PE   3: Inferred from homology;
KW   Calcium; Metal-binding; Reference proteome; Repeat.
FT   CHAIN           1..229
FT                   /note="Probable calcium-binding protein CML22"
FT                   /id="PRO_0000342951"
FT   DOMAIN          53..88
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          89..124
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          145..180
FT                   /note="EF-hand 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          184..219
FT                   /note="EF-hand 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         66
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         68
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         70
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         72
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         77
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ   SEQUENCE   229 AA;  26266 MW;  94C011172CA33740 CRC64;
     MLCCCVNSEN NKKYAELDAK LARKMVESRS IYPGHRSLKS MDSIIMKFPK LREGLRNIRS
     VFESYDNDTN GTIDIEELKK CLEELKLSLS DEEVKGLYSW CDVDGSKGIQ FNEFIVLLCL
     IYLLAKPSSE SSTESREMGP KLVESIFDPI VEVFLFLDKD GKGKLNKADV IKTLNNEDYP
     LERSPSHVTN MRFEEMDWGR KGKVGFREFL FAFMSWVGLD DADDDFMSS