CML49_ARATH
ID CML49_ARATH Reviewed; 335 AA.
AC Q8W4L0; Q2V3X2; Q3E6V8; Q9SS41;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Probable calcium-binding protein CML49;
DE AltName: Full=Calmodulin-like protein 49;
GN Name=CML49; OrderedLocusNames=At3g10300; ORFNames=F14P13.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1046/j.1469-8137.2003.00845.x;
RA McCormack E., Braam J.;
RT "Calmodulins and related potential calcium sensors of Arabidopsis.";
RL New Phytol. 159:585-598(2003).
RN [6]
RP INDUCTION.
RX PubMed=15720654; DOI=10.1111/j.1469-8137.2004.01238.x;
RA Lee D., Polisensky D.H., Braam J.;
RT "Genome-wide identification of touch- and darkness-regulated Arabidopsis
RT genes: a focus on calmodulin-like and XTH genes.";
RL New Phytol. 165:429-444(2005).
CC -!- FUNCTION: Potential calcium sensor. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8W4L0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8W4L0-2; Sequence=VSP_034560, VSP_034561;
CC Name=3;
CC IsoId=Q8W4L0-3; Sequence=VSP_034557, VSP_034558;
CC Name=4;
CC IsoId=Q8W4L0-4; Sequence=VSP_034559;
CC -!- INDUCTION: By touch and during darkness conditions.
CC {ECO:0000269|PubMed:15720654}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 3]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 4]: May be due to a competing donor splice site
CC and an exon skipping. {ECO:0000305}.
CC -!- CAUTION: Although assigned as a calmodulin family member by Ref.5, it
CC only contains EF-hand domains. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF02826.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC009400; AAF02826.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74885.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74886.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74887.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74888.1; -; Genomic_DNA.
DR EMBL; AY062498; AAL32576.1; -; mRNA.
DR EMBL; BT015172; AAT85768.1; -; mRNA.
DR RefSeq; NP_001030668.1; NM_001035591.2. [Q8W4L0-4]
DR RefSeq; NP_187641.2; NM_111865.7. [Q8W4L0-1]
DR RefSeq; NP_850997.1; NM_180666.4. [Q8W4L0-3]
DR RefSeq; NP_850998.1; NM_180667.4. [Q8W4L0-2]
DR AlphaFoldDB; Q8W4L0; -.
DR SMR; Q8W4L0; -.
DR STRING; 3702.AT3G10300.3; -.
DR PaxDb; Q8W4L0; -.
DR PRIDE; Q8W4L0; -.
DR EnsemblPlants; AT3G10300.1; AT3G10300.1; AT3G10300. [Q8W4L0-3]
DR EnsemblPlants; AT3G10300.2; AT3G10300.2; AT3G10300. [Q8W4L0-2]
DR EnsemblPlants; AT3G10300.3; AT3G10300.3; AT3G10300. [Q8W4L0-1]
DR EnsemblPlants; AT3G10300.4; AT3G10300.4; AT3G10300. [Q8W4L0-4]
DR GeneID; 820192; -.
DR Gramene; AT3G10300.1; AT3G10300.1; AT3G10300. [Q8W4L0-3]
DR Gramene; AT3G10300.2; AT3G10300.2; AT3G10300. [Q8W4L0-2]
DR Gramene; AT3G10300.3; AT3G10300.3; AT3G10300. [Q8W4L0-1]
DR Gramene; AT3G10300.4; AT3G10300.4; AT3G10300. [Q8W4L0-4]
DR KEGG; ath:AT3G10300; -.
DR Araport; AT3G10300; -.
DR TAIR; locus:2076229; AT3G10300.
DR eggNOG; KOG0037; Eukaryota.
DR HOGENOM; CLU_051357_3_0_1; -.
DR InParanoid; Q8W4L0; -.
DR OMA; MAYQGGY; -.
DR PhylomeDB; Q8W4L0; -.
DR PRO; PR:Q8W4L0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8W4L0; baseline and differential.
DR Genevisible; Q8W4L0; AT.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR InterPro; IPR044590; CML48/49/50.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR PANTHER; PTHR46824; PTHR46824; 1.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13405; EF-hand_6; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..335
FT /note="Probable calcium-binding protein CML49"
FT /id="PRO_0000342972"
FT DOMAIN 164..199
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 230..265
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..49
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 177
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 179
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 181
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 188
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 243
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 245
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 247
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 249
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 254
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT VAR_SEQ 222..232
FT /note="PKEFTSLFFSL -> QFLLSFKLISN (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_034557"
FT VAR_SEQ 233..335
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_034558"
FT VAR_SEQ 296..335
FT /note="CCLTVKGLTEKFKEKDTALSGSAIFNYENFMLTVLPFLVA -> YVHSGSPR
FT SSRRRIRRYQAQLFSITRTSCSLFYHSSSLE (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_034559"
FT VAR_SEQ 296..324
FT /note="CCLTVKGLTEKFKEKDTALSGSAIFNYEN -> YVHSFQPPYLFLLCNKYNS
FT SLILVYFPLM (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_034560"
FT VAR_SEQ 325..335
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_034561"
SQ SEQUENCE 335 AA; 35302 MW; A4571A9D713BE343 CRC64;
MSGYPPSSQG YGYGGNPPPP QPPYGSTGNN PPPYGSSGSN PPPPYGSSAS SPYAVPYGAQ
PAPYGAPPSA PYASLPGDHN KPHKEKPHGA SYGSPSPGGY GAHPSSGPSD YGGYGGAPQQ
SGHGGGYGGA PQQSGHGGGY GAPPPQASYG SPFASLVPSA FPPGTDPNIV ACFQAADRDN
SGFIDDKELQ GALSSYNQSF SIRTVHLLMY LFTNSNVRKI GPKEFTSLFF SLQNWRSIFE
RFDKDRSGRI DTNELRDALM SLGFSVSPVI LDLLVSKFDK SGGRNRAIEY DNFIECCLTV
KGLTEKFKEK DTALSGSAIF NYENFMLTVL PFLVA
