CMLA_PSEAI
ID CMLA_PSEAI Reviewed; 419 AA.
AC P32482; Q56411; Q56412;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Chloramphenicol resistance protein;
GN Name=cmlA;
OS Pseudomonas aeruginosa.
OG Plasmid R1033.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TRANSPOSON=Tn1696;
RX PubMed=1648560; DOI=10.1128/jb.173.14.4493-4502.1991;
RA Bissonnette L., Champetier S., Buisson J.-P., Roy P.H.;
RT "Characterization of the nonenzymatic chloramphenicol resistance (cmlA)
RT gene of the In4 integron of Tn1696: similarity of the product to
RT transmembrane transport proteins.";
RL J. Bacteriol. 173:4493-4502(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TRANSPOSON=Tn1696;
RX PubMed=1658833; DOI=10.1016/0147-619x(91)90032-r;
RA Stokes H.W., Hall R.M.;
RT "Sequence analysis of the inducible chloramphenicol resistance determinant
RT in the Tn1696 integron suggests regulation by translational attenuation.";
RL Plasmid 26:10-19(1991).
RN [3]
RP SEQUENCE REVISION.
RA Partridge S., Hall R.M.;
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Appears to provoke a reduction of the content of the major
CC porins OmpA and OmpC.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Bcr/CmlA
CC family. {ECO:0000305}.
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DR EMBL; M64556; AAA26057.1; -; Genomic_DNA.
DR EMBL; U12338; AAB60004.2; -; Genomic_DNA.
DR PIR; A47033; A47033.
DR RefSeq; WP_000095725.1; NZ_JTYV01000103.1.
DR RefSeq; WP_032488514.1; NG_047646.1.
DR AlphaFoldDB; P32482; -.
DR SMR; P32482; -.
DR TCDB; 2.A.1.2.3; the major facilitator superfamily (mfs).
DR KEGG; ag:AAB60004; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IEA:InterPro.
DR InterPro; IPR004812; Efflux_drug-R_Bcr/CmlA.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00710; efflux_Bcr_CflA; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Plasmid; Transmembrane; Transmembrane helix; Transport;
KW Transposable element.
FT CHAIN 1..419
FT /note="Chloramphenicol resistance protein"
FT /id="PRO_0000173320"
FT TOPO_DOM 1..9
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..80
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..109
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..127
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..141
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..169
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..187
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..214
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..245
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..279
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..299
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..306
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..327
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 328..342
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..370
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 392..419
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CONFLICT 412
FT /note="E -> G (in Ref. 1; AAA26057)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 419 AA; 44316 MW; EFBF1D97DD7B979B CRC64;
MSSKNFSWRY SLAATVLLLS PFDLLASLGM DMYLPAVPFM PNALGTTAST IQLTLTTYLV
MIGAGQLLFG PLSDRLGRRP VLLGGGLAYV VASMGLALTS SAEVFLGLRI LQACGASACL
VSTFATVRDI YAGREESNVI YGILGSMLAM VPAVGPLLGA LVDMWLGWRA IFAFLGLGMI
AASAAAWRFW PETRVQRVAG LQWSQLLLPV KCLNFWLYTL CYAAGMGSFF VFFSIAPGLM
MGRQGVSQLG FSLLFATVAI AMVFTARFMG RVIPKWGSPS VLRMGMGCLI AGAVLLAITE
IWALQSVLGF IAPMWLVGIG VATAVSVAPN GALRGFDHVA GTVTAVYFCL GGVLLGSIGT
LIISLLPRNT AWPVVVYCLT LATVVLGLSC VSRVKGSRGQ GEHDVVALQS AESTSNPNR
