CMLE_NEUCR
ID CMLE_NEUCR Reviewed; 366 AA.
AC P38677; Q7RV80;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Carboxy-cis,cis-muconate cyclase;
DE EC=5.5.1.5;
DE AltName: Full=3-carboxy-cis,cis-muconate lactonizing enzyme;
DE Short=CMLE;
GN ORFNames=NCU04071;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-41, AND CHARACTERIZATION.
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=8132467; DOI=10.1128/jb.176.6.1718-1728.1994;
RA Mazur P., Henzel W.J., Mattoo S., Kozarich J.W.;
RT "3-carboxy-cis,cis-muconate lactonizing enzyme from Neurospora crassa: an
RT alternate cycloisomerase motif.";
RL J. Bacteriol. 176:1718-1728(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX PubMed=11937053; DOI=10.1016/s0969-2126(02)00744-x;
RA Kajander T., Merckel M.C., Thompson A., Deacon A.M., Mazur P.,
RA Kozarich J.W., Goldman A.;
RT "The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing
RT enzyme, a beta propeller cycloisomerase.";
RL Structure 10:483-492(2002).
CC -!- FUNCTION: Catalyzes a syn cycloisomerization. Also possesses mle
CC activity.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 3-carboxy-
CC cis,cis-muconate; Xref=Rhea:RHEA:14977, ChEBI:CHEBI:57496,
CC ChEBI:CHEBI:57976; EC=5.5.1.5;
CC -!- PATHWAY: Aromatic compound metabolism; beta-ketoadipate pathway; 3-
CC carboxy-cis,cis-muconate from 3-carboxy-2,5-dihydro-5-oxofuran-2-
CC acetate: step 1/1.
CC -!- SUBUNIT: Homotetramer.
CC -!- SIMILARITY: Belongs to the cycloisomerase 2 family. {ECO:0000305}.
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DR EMBL; L27538; AAA21020.1; -; mRNA.
DR EMBL; CM002241; EAA28450.1; -; Genomic_DNA.
DR PIR; A55525; A55525.
DR RefSeq; XP_957686.1; XM_952593.2.
DR PDB; 1JOF; X-ray; 2.50 A; A/B/C/D/E/F/G/H=2-366.
DR PDBsum; 1JOF; -.
DR AlphaFoldDB; P38677; -.
DR SMR; P38677; -.
DR EnsemblFungi; EAA28450; EAA28450; NCU04071.
DR GeneID; 3873776; -.
DR KEGG; ncr:NCU04071; -.
DR VEuPathDB; FungiDB:NCU04071; -.
DR HOGENOM; CLU_045869_0_0_1; -.
DR InParanoid; P38677; -.
DR OMA; IFTVQFD; -.
DR UniPathway; UPA00157; UER00308.
DR EvolutionaryTrace; P38677; -.
DR Proteomes; UP000001805; Chromosome 5, Linkage Group VI.
DR GO; GO:0017057; F:6-phosphogluconolactonase activity; IBA:GO_Central.
DR GO; GO:0047768; F:carboxy-cis,cis-muconate cyclase activity; IEA:UniProtKB-EC.
DR GO; GO:0042952; P:beta-ketoadipate pathway; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR019405; Lactonase_7-beta_prop.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF10282; Lactonase; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Isomerase; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:8132467"
FT CHAIN 2..366
FT /note="Carboxy-cis,cis-muconate cyclase"
FT /id="PRO_0000171127"
FT ACT_SITE 149
FT ACT_SITE 197
FT ACT_SITE 213
FT ACT_SITE 275
FT STRAND 3..14
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 16..23
FT /evidence="ECO:0007829|PDB:1JOF"
FT TURN 24..27
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 28..36
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 44..48
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 52..60
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 62..70
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 73..81
FT /evidence="ECO:0007829|PDB:1JOF"
FT HELIX 86..89
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 96..102
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 110..118
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 121..127
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 133..141
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 148..153
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 157..164
FT /evidence="ECO:0007829|PDB:1JOF"
FT TURN 165..168
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 169..175
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 181..188
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 196..201
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 205..212
FT /evidence="ECO:0007829|PDB:1JOF"
FT TURN 213..216
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 217..223
FT /evidence="ECO:0007829|PDB:1JOF"
FT TURN 225..227
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 230..240
FT /evidence="ECO:0007829|PDB:1JOF"
FT TURN 249..251
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 252..263
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 267..278
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 284..290
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 296..304
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 315..317
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 324..328
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 330..332
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 334..341
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 344..352
FT /evidence="ECO:0007829|PDB:1JOF"
FT STRAND 358..365
FT /evidence="ECO:0007829|PDB:1JOF"
SQ SEQUENCE 366 AA; 41329 MW; E71BA50509E72873 CRC64;
MPLHHLMIGT WTPPGAIFTV QFDDEKLTCK LIKRTEIPQD EPISWMTFDH ERKNIYGAAM
KKWSSFAVKS PTEIVHEASH PIGGHPRAND ADTNTRAIFL LAAKQPPYAV YANPFYKFAG
YGNVFSVSET GKLEKNVQNY EYQENTGIHG MVFDPTETYL YSADLTANKL WTHRKLASGE
VELVGSVDAP DPGDHPRWVA MHPTGNYLYA LMEAGNRICE YVIDPATHMP VYTHHSFPLI
PPGIPDRDPE TGKGLYRADV CALTFSGKYM FASSRANKFE LQGYIAGFKL RDCGSIEKQL
FLSPTPTSGG HSNAVSPCPW SDEWMAITDD QEGWLEIYRW KDEFLHRVAR VRIPEPGFGM
NAIWYD
