CMOB_PSESM
ID CMOB_PSESM Reviewed; 319 AA.
AC Q87XG5;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=tRNA U34 carboxymethyltransferase {ECO:0000255|HAMAP-Rule:MF_01590};
DE EC=2.5.1.- {ECO:0000255|HAMAP-Rule:MF_01590};
GN Name=cmoB {ECO:0000255|HAMAP-Rule:MF_01590}; OrderedLocusNames=PSPTO_4213;
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC -!- FUNCTION: Catalyzes carboxymethyl transfer from carboxy-S-adenosyl-L-
CC methionine (Cx-SAM) to 5-hydroxyuridine (ho5U) to form 5-
CC carboxymethoxyuridine (cmo5U) at position 34 in tRNAs.
CC {ECO:0000255|HAMAP-Rule:MF_01590}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-hydroxyuridine(34) in tRNA + carboxy-S-adenosyl-L-methionine
CC = 5-carboxymethoxyuridine(34) in tRNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:52848, Rhea:RHEA-COMP:13381, Rhea:RHEA-
CC COMP:13383, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:134278,
CC ChEBI:CHEBI:136877, ChEBI:CHEBI:136879; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01590};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01590}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. CmoB family. {ECO:0000255|HAMAP-Rule:MF_01590}.
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DR EMBL; AE016853; AAO57669.1; -; Genomic_DNA.
DR RefSeq; NP_793974.1; NC_004578.1.
DR RefSeq; WP_005764771.1; NC_004578.1.
DR PDB; 4P7C; X-ray; 1.85 A; A/B=1-319.
DR PDBsum; 4P7C; -.
DR AlphaFoldDB; Q87XG5; -.
DR SMR; Q87XG5; -.
DR STRING; 223283.PSPTO_4213; -.
DR DNASU; 1185893; -.
DR EnsemblBacteria; AAO57669; AAO57669; PSPTO_4213.
DR GeneID; 1185893; -.
DR KEGG; pst:PSPTO_4213; -.
DR PATRIC; fig|223283.9.peg.4320; -.
DR eggNOG; COG0500; Bacteria.
DR HOGENOM; CLU_052665_0_0_6; -.
DR OMA; CEWRSDF; -.
DR OrthoDB; 1515497at2; -.
DR PhylomeDB; Q87XG5; -.
DR Proteomes; UP000002515; Chromosome.
DR GO; GO:0016765; F:transferase activity, transferring alkyl or aryl (other than methyl) groups; IEA:UniProtKB-UniRule.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_01590; tRNA_carboxymethyltr_CmoB; 1.
DR InterPro; IPR010017; CmoB.
DR InterPro; IPR027555; Mo5U34_MeTrfas-like.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF08003; Methyltransf_9; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR00452; TIGR00452; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Transferase; tRNA processing.
FT CHAIN 1..319
FT /note="tRNA U34 carboxymethyltransferase"
FT /id="PRO_0000313953"
FT BINDING 88
FT /ligand="carboxy-S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:134278"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01590"
FT BINDING 102
FT /ligand="carboxy-S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:134278"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01590"
FT BINDING 107
FT /ligand="carboxy-S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:134278"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01590"
FT BINDING 126
FT /ligand="carboxy-S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:134278"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01590"
FT BINDING 176..177
FT /ligand="carboxy-S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:134278"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01590"
FT BINDING 192
FT /ligand="carboxy-S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:134278"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01590"
FT BINDING 196
FT /ligand="carboxy-S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:134278"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01590"
FT BINDING 311
FT /ligand="carboxy-S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:134278"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01590"
FT HELIX 5..11
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 17..33
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 35..39
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 42..47
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 54..57
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 70..81
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 91..93
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 96..98
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 104..111
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 121..125
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 131..138
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 142..147
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 151..161
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 167..172
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 176..178
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 186..193
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 195..197
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 201..209
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 212..223
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 225..227
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 242..244
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 248..250
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 251..260
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 264..273
FT /evidence="ECO:0007829|PDB:4P7C"
FT TURN 276..278
FT /evidence="ECO:0007829|PDB:4P7C"
FT HELIX 290..293
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 298..302
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 305..307
FT /evidence="ECO:0007829|PDB:4P7C"
FT STRAND 310..317
FT /evidence="ECO:0007829|PDB:4P7C"
SQ SEQUENCE 319 AA; 36133 MW; 4DBD8E3C8D809418 CRC64;
MIDLAPLVRR LAGTPLAEWA NGLQAQLDTK MSKGHGDLQR WQSALDALPA LQPEKVDLTD
SFTLETECDG ETRTVLRKAL LGLSPWRKGP FNVFGVHIDT EWRSDWKWSR VSPHLDLKGK
RVLDVGCGNG YYQWRMLGAG ADSVIGVDPN WLFFCQFQAM QRYLPDLPAW HLPFALEDLP
ANLEGFDTVF SMGVLYHRKS PIDHLLALKD CLVKGGELVM ETLVIPGDVH QVLVPEDRYA
QMRNVWFLPS VPALELWMRR AGFTDVRCVD VSHTTVEEQR STEWMRFQSL GDYLDPNDHS
KTVEGLPAPM RAVIVGRKP
