CMPA_BACSU
ID CMPA_BACSU Reviewed; 214 AA.
AC P14204;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Transcriptional regulatory protein ComA;
DE AltName: Full=Competence protein A;
GN Name=comA; Synonyms=comA1, comAA; OrderedLocusNames=BSU31680;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2507523; DOI=10.1128/jb.171.10.5362-5375.1989;
RA Weinrauch Y., Guillen N., Dubnau D.;
RT "Sequence and transcription mapping of Bacillus subtilis competence genes
RT comB and comA, one of which is related to a family of bacterial regulatory
RT determinants.";
RL J. Bacteriol. 171:5362-5375(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9274030; DOI=10.1099/00221287-143-8-2769;
RA Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H.,
RA Duesterhoeft A., Pohl T.M., Weitzenegger T.;
RT "Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence
RT of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).";
RL Microbiology 143:2769-2774(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION.
RX PubMed=16091051; DOI=10.1111/j.1365-2958.2005.04749.x;
RA Comella N., Grossman A.D.;
RT "Conservation of genes and processes controlled by the quorum response in
RT bacteria: characterization of genes controlled by the quorum-sensing
RT transcription factor ComA in Bacillus subtilis.";
RL Mol. Microbiol. 57:1159-1174(2005).
CC -!- FUNCTION: Response regulator in the two-component regulatory system
CC ComP/ComA involved in a major quorum response pathway that regulates
CC the development of genetic competence. Regulates directly the
CC expression of over 20 genes, including genes of the srfA operon, degQ,
CC rapA, rapC, rapE, rapF, etc. Regulates indirectly, through the
CC regulation of comK transcription, the expression of late competence
CC genes. {ECO:0000269|PubMed:16091051}.
CC -!- INTERACTION:
CC P14204; P71002: rapF; NbExp=5; IntAct=EBI-6418022, EBI-15961797;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by ComP.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M22856; AAA22320.1; -; Genomic_DNA.
DR EMBL; Z93932; CAB07904.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15156.1; -; Genomic_DNA.
DR PIR; A33591; RGBSCA.
DR RefSeq; NP_391046.1; NC_000964.3.
DR RefSeq; WP_003220716.1; NZ_JNCM01000033.1.
DR PDB; 2KRF; NMR; -; A/B=146-214.
DR PDB; 3ULQ; X-ray; 2.30 A; B=146-214.
DR PDBsum; 2KRF; -.
DR PDBsum; 3ULQ; -.
DR AlphaFoldDB; P14204; -.
DR BMRB; P14204; -.
DR SMR; P14204; -.
DR DIP; DIP-59430N; -.
DR IntAct; P14204; 2.
DR STRING; 224308.BSU31680; -.
DR PaxDb; P14204; -.
DR PRIDE; P14204; -.
DR EnsemblBacteria; CAB15156; CAB15156; BSU_31680.
DR GeneID; 64304913; -.
DR GeneID; 937179; -.
DR KEGG; bsu:BSU31680; -.
DR PATRIC; fig|224308.179.peg.3433; -.
DR eggNOG; COG2197; Bacteria.
DR InParanoid; P14204; -.
DR OMA; MVFHYLA; -.
DR PhylomeDB; P14204; -.
DR BioCyc; BSUB:BSU31680-MON; -.
DR EvolutionaryTrace; P14204; -.
DR PRO; PR:P14204; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00196; GerE; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS00622; HTH_LUXR_1; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Competence; Cytoplasm; DNA-binding;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..214
FT /note="Transcriptional regulatory protein ComA"
FT /id="PRO_0000081075"
FT DOMAIN 3..121
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 147..212
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 171..190
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT MOD_RES 55
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT STRAND 150..154
FT /evidence="ECO:0007829|PDB:2KRF"
FT HELIX 156..166
FT /evidence="ECO:0007829|PDB:3ULQ"
FT HELIX 171..178
FT /evidence="ECO:0007829|PDB:3ULQ"
FT HELIX 182..195
FT /evidence="ECO:0007829|PDB:3ULQ"
FT HELIX 201..208
FT /evidence="ECO:0007829|PDB:3ULQ"
SQ SEQUENCE 214 AA; 24129 MW; 52D01A9740759072 CRC64;
MKKILVIDDH PAVMEGTKTI LETDSNLSVD CLSPEPSEQF IKQHDFSSYD LILMDLNLGG
EVNGMELSKQ ILQENPHCKI IVYTGYEVED YFEEAIRAGL HGAISKTESK EKITQYIYHV
LNGEILVDFA YFKQLMTQQK TKPAPSSQKE QDVLTPRECL ILQEVEKGFT NQEIADALHL
SKRSIEYSLT SIFNKLNVGS RTEAVLIAKS DGVL
