ID   CMR1B_PYRFU             Reviewed;         187 AA.
AC   Q8U3V3;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=CRISPR system Cmr subunit Cmr1-2;
DE   AltName: Full=CRISPR type III-B/RAMP module RAMP protein Cmr1-2;
GN   Name=cmr1-2 {ECO:0000303|PubMed:19945378}; OrderedLocusNames=PF0352;
OS   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=186497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA   Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA   DiRuggiero J., Robb F.T.;
RT   "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT   horikoshii inferred from complete genomic sequences.";
RL   Genetics 152:1299-1305(1999).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION IN CMR COMPLEX, SUBCELLULAR
RP   LOCATION, AND SUBUNIT.
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=19945378; DOI=10.1016/j.cell.2009.07.040;
RA   Hale C.R., Zhao P., Olson S., Duff M.O., Graveley B.R., Wells L.,
RA   Terns R.M., Terns M.P.;
RT   "RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex.";
RL   Cell 139:945-956(2009).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA),
CC       formerly called psiRNA (prokaryotic silencing) in this organism. Part
CC       of the Cmr ribonucleoprotein complex which has divalent cation-
CC       dependent endoribonuclease activity specific for ssRNA complementary to
CC       the crRNA (target RNA), generating 5' hydroxy- and 3' phosphate or 2'-
CC       3' cyclic phosphate termini (PubMed:19945378). Cmr4 is probably the
CC       subunit that cleaves target RNA. Cmr complex does not cleave ssDNA
CC       complementary to the crRNA. Cleavage of invading RNA is guided by the
CC       crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3'
CC       end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not
CC       absolutely necessary for target cleavage.
CC       {ECO:0000250|UniProtKB:Q8U1S5, ECO:0000269|PubMed:19945378}.
CC   -!- SUBUNIT: Part of the type III-B Cmr ribonucleoprotein (RNP) complex
CC       (PubMed:19945378). This is an elongated RNP with Cmr2 and Cmr3 as the
CC       base, with Cmr4 and Cmr5 forming a helical core along the mature crRNA
CC       (39 or 45 nt in length), while the complex is capped by Cmr6 and Cmr1.
CC       The 5' end of the crRNA is bound to Cmr2 and Cmr3, while Cmr6 and a
CC       Cmr1 subunit (Cmr1-1 or Cmr1-2) cap the 3' end of the crRNA. The target
CC       RNA lies antiparallel to the crRNA, with its 5' end near Cmr1 and Cmr6
CC       and its 3' end near Cmr2 and Cmr3; major target cleavage occurs nears
CC       the junction of Cmr1/Cmr6 and Cmr4/Cmr, with minor cleavage occurring
CC       at 6 nt intervals which coincide with the proposed spacing of Cmr4
CC       subunits. {ECO:0000250|UniProtKB:Q8U1S5, ECO:0000269|PubMed:19945378}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19945378}.
CC   -!- SIMILARITY: Belongs to the CRISPR system Cmr1 family. {ECO:0000305}.
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DR   EMBL; AE009950; AAL80476.1; -; Genomic_DNA.
DR   RefSeq; WP_048059033.1; NC_003413.1.
DR   AlphaFoldDB; Q8U3V3; -.
DR   SMR; Q8U3V3; -.
DR   IntAct; Q8U3V3; 1.
DR   STRING; 186497.PF0352; -.
DR   EnsemblBacteria; AAL80476; AAL80476; PF0352.
DR   GeneID; 1468187; -.
DR   KEGG; pfu:PF0352; -.
DR   PATRIC; fig|186497.12.peg.365; -.
DR   eggNOG; arCOG03891; Archaea.
DR   HOGENOM; CLU_1444720_0_0_2; -.
DR   Proteomes; UP000001013; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR007522; CRISPR-assoc_prot_TM1795.
DR   InterPro; IPR005537; RAMP_III_fam.
DR   Pfam; PF03787; RAMPs; 1.
DR   TIGRFAMs; TIGR01894; cas_TM1795_cmr1; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cytoplasm; Reference proteome.
FT   CHAIN           1..187
FT                   /note="CRISPR system Cmr subunit Cmr1-2"
FT                   /id="PRO_0000418071"
SQ   SEQUENCE   187 AA;  21719 MW;  B0D3699E70C929E3 CRC64;
     MYEAIFDLEA ITPLFMRGAD ARSPEFSSAS VKGVMRWWFR ALAGGYFGNN IEALKEVEEK
     IFGSTRNKSR VFVRAEVEDV KKGNIYRQAS SWADKTIIVW SEYVDYFFFS VLDKRRNRKT
     KKIDIKTRFE YFDVGSKFSI SLSSTDERYF RLAEASLWMT INLGGFGFRA RRGAGSLKVQ
     MLREMLL