ID   CMR1_SACS2              Reviewed;         467 AA.
AC   Q97WX2;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=CRISPR system CMR subunit Cmr1;
DE   AltName: Full=Type III-B/RAMP module RAMP protein Cmr1;
GN   Name=cmr1; OrderedLocusNames=SSO1989;
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION IN CMR COMPLEX, SUBUNIT, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=22227115; DOI=10.1016/j.molcel.2011.12.013;
RA   Zhang J., Rouillon C., Kerou M., Reeks J., Brugger K., Graham S.,
RA   Reimann J., Cannone G., Liu H., Albers S.V., Naismith J.H., Spagnolo L.,
RA   White M.F.;
RT   "Structure and mechanism of the CMR complex for CRISPR-mediated antiviral
RT   immunity.";
RL   Mol. Cell 45:303-313(2012).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA) (By
CC       similarity). The CMR complex degrades RNA complementary to the crRNA
CC       (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate
CC       ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an
CC       unpaired 3' flag on the target RNA, and is stimulated by ATP. Some
CC       cleavage of the guide crRNA can also be observed. {ECO:0000250,
CC       ECO:0000269|PubMed:22227115}.
CC   -!- SUBUNIT: Part of the CMR ribonucleoprotein complex, consisting of crRNA
CC       plus Cmr1/Cmr2/Cmr3/Cmr4/Cmr5/Cmr6 at 1:1 and possibly 3 Cmr7 dimers. A
CC       Cmr2/Cmr3/Cmr7 subcomplex without crRNA can also be isolated. It does
CC       not cleave target RNA. {ECO:0000269|PubMed:22227115}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22227115}.
CC   -!- SIMILARITY: Belongs to the CRISPR system Cmr1 family. {ECO:0000305}.
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DR   EMBL; AE006641; AAK42179.1; -; Genomic_DNA.
DR   RefSeq; WP_009992994.1; NC_002754.1.
DR   AlphaFoldDB; Q97WX2; -.
DR   SMR; Q97WX2; -.
DR   STRING; 273057.SSO1989; -.
DR   EnsemblBacteria; AAK42179; AAK42179; SSO1989.
DR   GeneID; 27428315; -.
DR   KEGG; sso:SSO1989; -.
DR   PATRIC; fig|273057.12.peg.2065; -.
DR   eggNOG; arCOG03893; Archaea.
DR   HOGENOM; CLU_584790_0_0_2; -.
DR   OMA; KWWTRIL; -.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR007522; CRISPR-assoc_prot_TM1795.
DR   InterPro; IPR005537; RAMP_III_fam.
DR   Pfam; PF03787; RAMPs; 1.
DR   TIGRFAMs; TIGR01894; cas_TM1795_cmr1; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cytoplasm; Reference proteome.
FT   CHAIN           1..467
FT                   /note="CRISPR system CMR subunit Cmr1"
FT                   /id="PRO_0000418072"
SQ   SEQUENCE   467 AA;  54844 MW;  A17CF7D8D11E4B35 CRC64;
     MEELLMSFKL KAIYPLTGGY NRHSINEFYE ENVRPTEIKG LWRWWNRVLF NTVSYVKEGK
     LYTYDSIDRL FEDVFGSENK KSAVRLEVIT DEGSDNHFEL SNVELDNVID CLKANREEKV
     NLDFRDNTLI IEIEGSTKIP ISFKSNLDID KIKDLVYKNK LLSFELLGFK SIKIDTKISD
     KEVIKEILRD LITNYLEYFN IKQEVTFTLN IYLDKSLKHK QNFDAKLKFA LHSLLVFILL
     GGIGRKTSRG FGGLSIVNAE CHDGLCGEIY GIVNNMESEK EKKDLATVLP NIIFSQTIEQ
     YFSELINNES YKLRSWNNNS DFFVYYFIKD INILRINRID TNVNRNGIEN ILNRISNELS
     ASGNCLKDLI MQEMRRRAFA LAFLGNRKFR NIHEIYPRIL EFLYANYIKR EFVNLIGKER
     RLSNLRFKIL EINNTYYIIS YLLYSSYLKD PNSSIKDTLY QFARCVI