ID   CMR4_SACS2              Reviewed;         308 AA.
AC   Q97WX4;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=CRISPR system CMR subunit Cmr4;
DE   AltName: Full=CRISPR type III-B/RAMP module RAMP protein Cmr4;
GN   Name=cmr4; OrderedLocusNames=SSO1987;
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION IN CMR COMPLEX, SUBUNIT, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=22227115; DOI=10.1016/j.molcel.2011.12.013;
RA   Zhang J., Rouillon C., Kerou M., Reeks J., Brugger K., Graham S.,
RA   Reimann J., Cannone G., Liu H., Albers S.V., Naismith J.H., Spagnolo L.,
RA   White M.F.;
RT   "Structure and mechanism of the CMR complex for CRISPR-mediated antiviral
RT   immunity.";
RL   Mol. Cell 45:303-313(2012).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA) (By
CC       similarity). The CMR complex degrades RNA complementary to the crRNA
CC       (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate
CC       ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an
CC       unpaired 3' flag on the target RNA, and is stimulated by ATP. Some
CC       cleavage of the guide crRNA can also be observed. {ECO:0000250,
CC       ECO:0000269|PubMed:22227115}.
CC   -!- SUBUNIT: Part of the CMR ribonucleoprotein complex, consisting of crRNA
CC       plus Cmr1/Cmr2/Cmr3/Cmr4/Cmr5/Cmr6 at 1:1 and possibly 3 Cmr7 dimers. A
CC       Cmr2/Cmr3/Cmr7 subcomplex without crRNA can also be isolated. It does
CC       not cleave target RNA. {ECO:0000269|PubMed:22227115}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22227115}.
CC   -!- SIMILARITY: Belongs to the CRISPR system Cmr4 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE006641; AAK42177.1; -; Genomic_DNA.
DR   PIR; B90365; B90365.
DR   AlphaFoldDB; Q97WX4; -.
DR   SMR; Q97WX4; -.
DR   STRING; 273057.SSO1987; -.
DR   EnsemblBacteria; AAK42177; AAK42177; SSO1987.
DR   KEGG; sso:SSO1987; -.
DR   PATRIC; fig|273057.12.peg.2063; -.
DR   eggNOG; arCOG02657; Archaea.
DR   HOGENOM; CLU_047795_0_0_2; -.
DR   InParanoid; Q97WX4; -.
DR   OMA; VINEMEF; -.
DR   PhylomeDB; Q97WX4; -.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR013410; CRISPR-assoc_RAMP_Cmr4.
DR   InterPro; IPR005537; RAMP_III_fam.
DR   PANTHER; PTHR36700; PTHR36700; 1.
DR   Pfam; PF03787; RAMPs; 1.
DR   TIGRFAMs; TIGR02580; cas_RAMP_Cmr4; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cytoplasm; Reference proteome.
FT   CHAIN           1..308
FT                   /note="CRISPR system CMR subunit Cmr4"
FT                   /id="PRO_0000418078"
SQ   SEQUENCE   308 AA;  34725 MW;  BF65DC97DD6A9222 CRC64;
     MIIMTYYTFV KPFFIKAVTH LHVSSGTSVE EEVDLPFQRD ELNYPTIFAS SLKGAIKSFL
     LKEYSNAREI IYKVLGSDEN PDEASLGSFL DAVLFAIPAR SIGVSGLDEA WVYVTTYELL
     RKVKSLLESM NTLSATQFTS LLDAINNILQ ESKNLIISDN AASVILNEDF YVELSNIQNE
     YIQSLLGDML IENSTYNQSN QNSQSQGNHL VKPLIVLKDS IGREVINRSL IRVRRIKIKR
     SSKTVEEGGL WSEEYVPVNS LFFSSFLARG SKDTAYFADC ILRKTRYLIL GGKETIGKGI
     VELRWLND