位置:首页 > 蛋白库 > CMT1_CRYNH
CMT1_CRYNH
ID   CMT1_CRYNH              Reviewed;         122 AA.
AC   J9VXB3;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2013, sequence version 2.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Copper metallothionein 1 {ECO:0000303|PubMed:21819456};
DE            Short=Cu-MT 1 {ECO:0000303|PubMed:21819456};
DE            Short=Cu-metallothionein 1 {ECO:0000303|PubMed:21819456};
DE   AltName: Full=Copper chelatin 1 {ECO:0000305};
DE   AltName: Full=Copper thionein 1 {ECO:0000305};
GN   Name=CMT1 {ECO:0000303|PubMed:23498952}; ORFNames=CNAG_05449;
OS   Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS   CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=235443;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX   PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA   Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V.,
RA   Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F.,
RA   Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A.,
RA   Gaillardin C., Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S.,
RA   Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L.,
RA   Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K.,
RA   Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A.,
RA   Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA   Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA   Cuomo C.A., Dietrich F.S.;
RT   "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT   grubii reveals complex RNA expression and microevolution leading to
RT   virulence attenuation.";
RL   PLoS Genet. 10:E1004261-E1004261(2014).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=21819456; DOI=10.1111/j.1365-2958.2011.07794.x;
RA   Ding C., Yin J., Tovar E.M., Fitzpatrick D.A., Higgins D.G., Thiele D.J.;
RT   "The copper regulon of the human fungal pathogen Cryptococcus neoformans
RT   H99.";
RL   Mol. Microbiol. 81:1560-1576(2011).
RN   [3]
RP   FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, DOMAIN, COPPER-BINDING, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=23498952; DOI=10.1016/j.chom.2013.02.002;
RA   Ding C., Festa R.A., Chen Y.L., Espart A., Palacios O., Espin J.,
RA   Capdevila M., Atrian S., Heitman J., Thiele D.J.;
RT   "Cryptococcus neoformans copper detoxification machinery is critical for
RT   fungal virulence.";
RL   Cell Host Microbe 13:265-276(2013).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=31694529; DOI=10.1186/s12866-019-1606-4;
RA   Dbouk N.H., Covington M.B., Nguyen K., Chandrasekaran S.;
RT   "Increase of reactive oxygen species contributes to growth inhibition by
RT   fluconazole in Cryptococcus neoformans.";
RL   BMC Microbiol. 19:243-243(2019).
CC   -!- FUNCTION: Copper metallothionein that protects the cell against copper
CC       toxicity by tightly chelating copper ions (PubMed:21819456,
CC       PubMed:23498952). Required for antioxidant-mediated growth rescue in
CC       the presence of fluconazole (PubMed:31694529). Acts as a critical
CC       factors for lung colonization and virulence (PubMed:23498952).
CC       {ECO:0000269|PubMed:21819456, ECO:0000269|PubMed:23498952,
CC       ECO:0000269|PubMed:31694529}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC       {ECO:0000269|PubMed:23498952}.
CC   -!- INDUCTION: Expression is induced in a dose-dependent manner in response
CC       to high concentrations of copper and basal transcription is repressed
CC       in the presence of the copper chelator bathocuproin sulphonate (BCS)
CC       (PubMed:21819456). Expression is regulated by the CUF1 copper-dependent
CC       transcription factor (PubMed:21819456). Highly induced during mice
CC       pulmonary infection (PubMed:23498952). {ECO:0000269|PubMed:21819456,
CC       ECO:0000269|PubMed:23498952}.
CC   -!- DOMAIN: Divided into 4 Cys-rich domains by 3 spacer sequences termed
CC       B1-B3 (PubMed:23498952). Each cystein-rich domain contains the
CC       conserved copper-binding motif Cys-X-Cys-X6-Cys-X-Cys-X4-Cys-X-Cys-X2-
CC       Cys (PubMed:23498952). The copper-binding domains together are able to
CC       chelate up to 16 copper ions (PubMed:23498952).
CC       {ECO:0000269|PubMed:23498952}.
CC   -!- DISRUPTION PHENOTYPE: Fungi lacking both CMT1 and CMT2 show attenuated
CC       virulence and reduced pulmonary colonization in mice (PubMed:23498952).
CC       Impairs growth rescue by antioxidants when cells are treated with
CC       fluconazole (PubMed:31694529). {ECO:0000269|PubMed:23498952,
CC       ECO:0000269|PubMed:31694529}.
CC   -!- SIMILARITY: Belongs to the metallothionein superfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP003833; AFR98878.2; -; Genomic_DNA.
DR   RefSeq; XP_012053609.1; XM_012198219.1.
DR   AlphaFoldDB; J9VXB3; -.
DR   EnsemblFungi; AFR98878; AFR98878; CNAG_05449.
DR   GeneID; 23888764; -.
DR   VEuPathDB; FungiDB:CNAG_05449; -.
DR   HOGENOM; CLU_146153_0_0_1; -.
DR   Proteomes; UP000010091; Chromosome 14.
DR   GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Copper; Cytoplasm; Metal-binding; Metal-thiolate cluster; Repeat.
FT   CHAIN           1..122
FT                   /note="Copper metallothionein 1"
FT                   /id="PRO_0000449496"
FT   REGION          1..35
FT                   /note="Cys-rich copper-binding 1"
FT                   /evidence="ECO:0000305|PubMed:23498952"
FT   REGION          36..50
FT                   /note="Spacer B1"
FT                   /evidence="ECO:0000305|PubMed:23498952"
FT   REGION          51..72
FT                   /note="Cys-rich copper-binding 2"
FT                   /evidence="ECO:0000305|PubMed:23498952"
FT   REGION          73..81
FT                   /note="Spacer B2"
FT                   /evidence="ECO:0000305|PubMed:23498952"
FT   REGION          82..103
FT                   /note="Cys-rich copper-binding 3"
FT                   /evidence="ECO:0000305|PubMed:23498952"
FT   REGION          104..113
FT                   /note="Spacer B3"
FT                   /evidence="ECO:0000305|PubMed:23498952"
FT   REGION          114..122
FT                   /note="Cys-rich copper-binding 4"
FT                   /evidence="ECO:0000305|PubMed:23498952"
SQ   SEQUENCE   122 AA;  11890 MW;  3B16615B4A78F718 CRC64;
     MACNCPPQKN TACCSTSEAQ DKCTCQKGNC ECKACPNSTK TSESGGKAST CNCGGSGEAC
     TCPPGQCACD KCPKKAKSVS TCGCGGSGAA CSCPPGKCAC DNCPKQAQEK VSSCACSGSG
     AA
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024