CMT2_ORYSJ
ID CMT2_ORYSJ Reviewed; 1319 AA.
AC Q5KQL9; A0A0P0WJF8; C0SQ91; Q0DJV2; Q5KQM0;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2016, sequence version 2.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=DNA (cytosine-5)-methyltransferase CMT2 {ECO:0000305};
DE EC=2.1.1.37 {ECO:0000305};
DE AltName: Full=Chromomethylase 2 {ECO:0000305};
DE AltName: Full=OsCMT2 {ECO:0000303|PubMed:19788421};
GN Name=CMT2 {ECO:0000305};
GN OrderedLocusNames=Os05g0224800/Os05g0224900 {ECO:0000312|EMBL:BAF16872.1},
GN LOC_Os05g13780/LOC_Os05g13790 {ECO:0000305};
GN ORFNames=OJ1732_H01.8 {ECO:0000312|EMBL:AAW56861.1},
GN OJ1732_H01.9 {ECO:0000312|EMBL:AAW56862.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-571.
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 545-1293.
RC STRAIN=cv. Nipponbare;
RX PubMed=26243209; DOI=10.1111/tpj.12952;
RA Cheng C., Tarutani Y., Miyao A., Ito T., Yamazaki M., Sakai H., Fukai E.,
RA Hirochika H.;
RT "Loss of function mutations in the rice chromomethylase OsCMT3a cause a
RT burst of transposition.";
RL Plant J. 83:1069-1081(2015).
RN [7]
RP INDUCTION.
RX PubMed=19788421; DOI=10.1111/j.1742-4658.2009.07338.x;
RA Sharma R., Mohan Singh R.K., Malik G., Deveshwar P., Tyagi A.K., Kapoor S.,
RA Kapoor M.;
RT "Rice cytosine DNA methyltransferases - gene expression profiling during
RT reproductive development and abiotic stress.";
RL FEBS J. 276:6301-6311(2009).
CC -!- FUNCTION: Involved in CpXpG DNA methylation.
CC {ECO:0000250|UniProtKB:C0SQ89}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-
CC methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:13681, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:11370,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:85452, ChEBI:CHEBI:85454; EC=2.1.1.37;
CC Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: By cold and salt stresses. {ECO:0000269|PubMed:19788421}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAW56861.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAW56862.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF16871.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF16872.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAS92885.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAS92886.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC098835; AAW56861.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC098835; AAW56862.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008211; BAF16871.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008211; BAF16872.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014961; BAS92885.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014961; BAS92886.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK109728; BAG98874.1; -; mRNA.
DR EMBL; AB360585; BAH37021.1; -; Genomic_DNA.
DR RefSeq; XP_015639449.1; XM_015783963.1.
DR AlphaFoldDB; Q5KQL9; -.
DR SMR; Q5KQL9; -.
DR STRING; 4530.OS05T0224900-01; -.
DR REBASE; 11924; M.OsaCMT2P.
DR iPTMnet; Q5KQL9; -.
DR PaxDb; Q5KQL9; -.
DR PRIDE; Q5KQL9; -.
DR GeneID; 4338140; -.
DR KEGG; osa:4338140; -.
DR eggNOG; ENOG502QT36; Eukaryota.
DR HOGENOM; CLU_475212_0_0_1; -.
DR OrthoDB; 898916at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IEA:InterPro.
DR GO; GO:0003886; F:DNA (cytosine-5-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR Gene3D; 2.30.30.490; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR001025; BAH_dom.
DR InterPro; IPR043151; BAH_sf.
DR InterPro; IPR018117; C5_DNA_meth_AS.
DR InterPro; IPR001525; C5_MeTfrase.
DR InterPro; IPR031303; C5_meth_CS.
DR InterPro; IPR016197; Chromo-like_dom_sf.
DR InterPro; IPR000953; Chromo/chromo_shadow_dom.
DR InterPro; IPR023780; Chromo_domain.
DR InterPro; IPR023779; Chromodomain_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF01426; BAH; 1.
DR Pfam; PF00385; Chromo; 1.
DR Pfam; PF00145; DNA_methylase; 1.
DR PRINTS; PR00105; C5METTRFRASE.
DR SMART; SM00439; BAH; 1.
DR SMART; SM00298; CHROMO; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR SUPFAM; SSF54160; SSF54160; 1.
DR PROSITE; PS51038; BAH; 1.
DR PROSITE; PS00094; C5_MTASE_1; 1.
DR PROSITE; PS00095; C5_MTASE_2; 1.
DR PROSITE; PS00598; CHROMO_1; 1.
DR PROSITE; PS50013; CHROMO_2; 1.
DR PROSITE; PS51679; SAM_MT_C5; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Methyltransferase; Nucleus; Reference proteome;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..1319
FT /note="DNA (cytosine-5)-methyltransferase CMT2"
FT /id="PRO_0000438157"
FT DOMAIN 602..719
FT /note="BAH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00370"
FT DOMAIN 758..1296
FT /note="SAM-dependent MTase C5-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01016"
FT DOMAIN 863..928
FT /note="Chromo"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00053"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 142..189
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 265..302
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 442..468
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 281..295
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 442..466
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 941
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01016"
SQ SEQUENCE 1319 AA; 144603 MW; 1802D8D9024F2FED CRC64;
METPPPDPVS PPPPAADEGS PGGDDGAEDA GGFSAGLDSL WTALFGSPEE LEPMWSPPRG
FGVGAEFAAA EVEPEIMDVA GGPWDGAPWR SSGVVAGEGA ATALVPPTAA AGFAEFEPAA
PIDSYPAGAA AASLGDVPEV SALDSGVDCS PDPPPSSSPP VDFDARGFDP VADSAPAMES
PLPPSVASSE ANLDGRMLDC TLNSVPSPPL ASPYEVGLGA EDPIKDSSPS VAWGTTMDAK
DPEVDATCAN GTALRRSRRI MKIKSAASSM PLNQNGDSSR ASKRRVADSR KSRSSEGSKL
PAFTGPISVN TVDLINGVKV QGLQEIVAVE NVSSSYDNNQ KAGGLYNQVV VALPAASNSL
LKDKGASVLP RRKTRLASKV LVNSDRVSAI SPVVNGGPPV QKSDVCIPTK KHKLAVEECL
TSLDGVDGGG IVLCNSKLKS AKSRVVSKTP QGRGRRSPQP PKTQRARTLS VKYLEKLKRA
ENNNNNGSMS KSPRVPMIPE NNGSMSKSPR VPIIPELSTK HELVLDKHMV DSVMLETDDG
SCFFVGDAVP DDEARKQWPH RYEINDQIMK KDKRTSSQTF ANAGKAVLDV KCHYLQAKVS
RYTFCIGECA FVKGPEGKPN YIGRLLEFFE TKTGECYFRV QWFFTAEDTV IGEQAQSHDP
RRLFYSDLTD DNLLDCIVSK VTIVQVPPSV DGKSKSVPSS DYYYDMKYSI DYSTFSTIEM
EDTDDLMQSC YTSRINDKMK KIDVNKKHKS PVLEKMELSL LDLYCGCGGM STGLCLGARG
GGVNLSARWA IDDDEIACES FRNNHPETRV RNETTDDFLE LLKEWEKLCK TYVKHSRTKA
CVDSTTESNN ETPDCSTVPP EEFEVWKLVD ICFGDPNKVS KHGLYFKVRW KGYGPHHDTW
EPVEGLRNCK EAIRDFVIEG HRQRILPRPG DVDVVCGGPP CQGISGYNRN REFEAPFKCE
KNKQIIVFMD VVQFLKPKYV YMENVLDILK FADATLARYA LSRLVAMHYQ ARLGIMAAGC
YGLPQFRMRV FLLGCHSKEK LPPFPLPTHE AIVKNGCPLA FERNLVGWPN DTPMQLARPI
VLEDILSDLP EVANGESRDE MLYVKGPQTE FQRYIRSFNV EVHGPRAHVT KDSKSSKLYD
HRPLVLDNDN YQRILQIPKR KGANFRDLSG VIVGPDNVAR LDPTKERVLL PSGRPLVLDC
ILAYENGKSL RPFGRVWWDE VVGTVLTVPN ARMQALIHPA QDRLLTIRES ARLQGFPDNY
RFRGTVKDRY RQIGNAVAVP VGRALGYALA MAYLKKSGDD PLMLLPPNFA FSHDLRGFA
