ID   CNBA_COMTE              Reviewed;         227 AA.
AC   Q5XW77;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Chloronitrobenzene nitroreductase {ECO:0000303|PubMed:16517619};
DE   AltName: Full=Nitrobenzene nitroreductase {ECO:0000305|PubMed:16517619};
DE            EC=1.7.1.16 {ECO:0000305|PubMed:16517619};
GN   Name=cnbA {ECO:0000303|PubMed:16517619};
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OG   Plasmid pCNB {ECO:0000312|EMBL:AAU43738.1}.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CNB-1; PLASMID=pCNB {ECO:0000312|EMBL:AAU43738.1};
RX   PubMed=15580337; DOI=10.1007/s00203-004-0738-5;
RA   Wu J.F., Sun C.W., Jiang C.Y., Liu Z.P., Liu S.J.;
RT   "A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-
RT   chloronitrobenzene by Comamonas strain CNB-1: purification, properties,
RT   genetic cloning and expression in Escherichia coli.";
RL   Arch. Microbiol. 183:1-8(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   PATHWAY.
RC   STRAIN=CNB-1; PLASMID=pCNB {ECO:0000312|EMBL:AAU43738.1};
RX   PubMed=16517619; DOI=10.1128/aem.72.3.1759-1765.2006;
RA   Wu J.F., Jiang C.Y., Wang B.J., Ma Y.F., Liu Z.P., Liu S.J.;
RT   "Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene
RT   degradation in Comamonas sp. strain CNB-1.";
RL   Appl. Environ. Microbiol. 72:1759-1765(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pCNB {ECO:0000312|EMBL:AAU43738.1};
RX   PubMed=17526790; DOI=10.1128/aem.00616-07;
RA   Ma Y.F., Wu J.F., Wang S.Y., Jiang C.Y., Zhang Y., Qi S.W., Liu L.,
RA   Zhao G.P., Liu S.J.;
RT   "Nucleotide sequence of plasmid pCNB1 from Comamonas strain CNB-1 reveals
RT   novel genetic organization and evolution for 4-chloronitrobenzene
RT   degradation.";
RL   Appl. Environ. Microbiol. 73:4477-4483(2007).
CC   -!- FUNCTION: Involved in the biodegradation of chlorinated nitroaromatic
CC       compounds. Catalyzes the reduction of 4-chloronitrobenzene to yield 1-
CC       hydroxylamino-4-chlorobenzene. Probably also able to catalyze the two-
CC       electron reduction of nitrobenzene (NB) to produce a nitrosobenzene
CC       (NOB) intermediate, which is immediately reduced to
CC       hydroxylaminobenzene (HAB) by a second two-electron transfer.
CC       {ECO:0000269|PubMed:16517619}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-phenylhydroxylamine + 2 NADP(+) = 2 H(+) + 2 NADPH +
CC         nitrobenzene; Xref=Rhea:RHEA:52884, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:27798, ChEBI:CHEBI:28902,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.7.1.16;
CC         Evidence={ECO:0000305|PubMed:16517619};
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000250|UniProtKB:A0R6D0};
CC       Note=Binds 1 FMN per subunit. {ECO:0000250|UniProtKB:A0R6D0};
CC   -!- PATHWAY: Xenobiotic degradation; nitrobenzene degradation.
CC       {ECO:0000305|PubMed:16517619}.
CC   -!- PATHWAY: Xenobiotic degradation; 4-chloronitrobenzene degradation.
CC       {ECO:0000305|PubMed:16517619}.
CC   -!- SIMILARITY: Belongs to the nitroreductase family. {ECO:0000305}.
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DR   EMBL; EF079106; AAU43738.1; -; Genomic_DNA.
DR   RefSeq; YP_001967716.1; NC_010935.1.
DR   AlphaFoldDB; Q5XW77; -.
DR   SMR; Q5XW77; -.
DR   PATRIC; fig|688245.4.peg.77; -.
DR   UniPathway; UPA00923; -.
DR   UniPathway; UPA01033; -.
DR   GO; GO:0018546; F:nitrobenzene nitroreductase activity; IEA:RHEA.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.109.10; -; 1.
DR   InterPro; IPR029479; Nitroreductase.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   Pfam; PF00881; Nitroreductase; 1.
DR   SUPFAM; SSF55469; SSF55469; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Flavoprotein; FMN; NADP;
KW   Nucleotide-binding; Oxidoreductase; Plasmid.
FT   CHAIN           1..227
FT                   /note="Chloronitrobenzene nitroreductase"
FT                   /id="PRO_0000441895"
FT   BINDING         14..18
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:A0R6D0"
FT   BINDING         44
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:A0R6D0"
FT   BINDING         172..173
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:A0R6D0"
FT   BINDING         215
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:A0R6D0"
SQ   SEQUENCE   227 AA;  25970 MW;  1271274BC394AB00 CRC64;
     MPTSPFIDDL IRDRRTKRGF LDQPVPIEMV KDILSVAKYT PSSSNTQPWR CYVLTGEARE
     RVTTAAVEAY RGAPEGLKPE YSYFPEPLHE PYATRFNSFR GQLGDAEGCC RSDITGRRRY
     VERQFRFFDA PVGLIFTMDR RLEWASFICY GCFLQNIMLA AKGRGLDTCP QGLWSLQHPV
     LRTELNLPDD QMVVAGMSLG WADNSMAVNQ MSMSRVELEE FTTFVHE