CNBL2_ORYSJ
ID CNBL2_ORYSJ Reviewed; 225 AA.
AC Q3HRP5; B9GE30; Q2QMP2;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Calcineurin B-like protein 2;
GN Name=CBL2; OrderedLocusNames=Os12g0597000, LOC_Os12g40510;
GN ORFNames=OsJ_36745 {ECO:0000312|EMBL:EEE53537.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Nipponbare; TISSUE=Seedling;
RX PubMed=18395997; DOI=10.1016/j.gene.2008.02.011;
RA Gu Z., Ma B., Jiang Y., Chen Z., Su X., Zhang H.;
RT "Expression analysis of the calcineurin B-like gene family in rice (Oryza
RT sativa L.) under environmental stresses.";
RL Gene 415:1-12(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14730064; DOI=10.1104/pp.103.033068;
RA Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., Kudla J.;
RT "Calcium sensors and their interacting protein kinases: genomics of the
RT Arabidopsis and rice CBL-CIPK signaling networks.";
RL Plant Physiol. 134:43-58(2004).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND GENE
RP FAMILY.
RX PubMed=15980189; DOI=10.1104/pp.105.062703;
RA Hwang Y.-S., Bethke P.C., Cheong Y.H., Chang H.-S., Zhu T., Jones R.L.;
RT "A gibberellin-regulated calcineurin B in rice localizes to the tonoplast
RT and is implicated in vacuole function.";
RL Plant Physiol. 138:1347-1358(2005).
CC -!- FUNCTION: Acts as a calcium sensor. May promote vacuolation of aleurone
CC cells in response to signal induced by gibberellin. CBL proteins
CC interact with CIPK serine-threonine protein kinases. Binding of a CBL
CC protein to the regulatory NAF domain of a CIPK protein lead to the
CC activation of the kinase in a calcium-dependent manner (By similarity).
CC {ECO:0000250, ECO:0000269|PubMed:15980189}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole, aleurone grain membrane
CC {ECO:0000269|PubMed:15980189}. Note=Tonoplast. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, shoots, culms, young spikelets
CC and scutellum. {ECO:0000269|PubMed:15980189,
CC ECO:0000269|PubMed:18395997}.
CC -!- INDUCTION: By gibberellin. {ECO:0000269|PubMed:15980189}.
CC -!- SIMILARITY: Belongs to the calcineurin regulatory subunit family.
CC {ECO:0000305}.
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DR EMBL; DQ201196; ABA54177.1; -; mRNA.
DR EMBL; DP000011; ABA99199.2; -; Genomic_DNA.
DR EMBL; DP000011; ABG22071.1; -; Genomic_DNA.
DR EMBL; AP008218; BAF30209.1; -; Genomic_DNA.
DR EMBL; AP014968; BAT17929.1; -; Genomic_DNA.
DR EMBL; CM000149; EEE53537.1; -; Genomic_DNA.
DR RefSeq; XP_015620792.1; XM_015765306.1.
DR AlphaFoldDB; Q3HRP5; -.
DR SMR; Q3HRP5; -.
DR STRING; 4530.OS12T0597000-01; -.
DR PaxDb; Q3HRP5; -.
DR PRIDE; Q3HRP5; -.
DR EnsemblPlants; Os12t0597000-01; Os12t0597000-01; Os12g0597000.
DR GeneID; 4352701; -.
DR Gramene; Os12t0597000-01; Os12t0597000-01; Os12g0597000.
DR KEGG; osa:4352701; -.
DR eggNOG; KOG0034; Eukaryota.
DR HOGENOM; CLU_061288_21_0_1; -.
DR InParanoid; Q3HRP5; -.
DR OMA; TQQGPDT; -.
DR OrthoDB; 1271942at2759; -.
DR Proteomes; UP000000763; Chromosome 12.
DR Proteomes; UP000007752; Chromosome 12.
DR Proteomes; UP000059680; Chromosome 12.
DR Genevisible; Q3HRP5; OS.
DR GO; GO:0032578; C:aleurone grain membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0019900; F:kinase binding; IEA:InterPro.
DR GO; GO:0019722; P:calcium-mediated signaling; IEA:InterPro.
DR InterPro; IPR045198; CNBL1-10.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR PANTHER; PTHR23056; PTHR23056; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR Pfam; PF13833; EF-hand_8; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Repeat; Vacuole.
FT CHAIN 1..225
FT /note="Calcineurin B-like protein 2"
FT /id="PRO_0000337765"
FT DOMAIN 45..80
FT /note="EF-hand 1"
FT /evidence="ECO:0000305"
FT DOMAIN 81..116
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 118..153
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 162..197
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT SITE 154
FT /note="Involved in dimerization"
FT /evidence="ECO:0000250"
SQ SEQUENCE 225 AA; 25865 MW; 90DF6F7D584CDC84 CRC64;
MVQCLDGVRQ LLAVVFKCCD LELKQPRGLE DPQVLARETV FSVSEVEALY ELFKKISSAV
IDDGLINKEE FQLALFKTSK KESLFADRVF DLFDTKHNGI LGFDEFARAL SVFHPSAPLD
EKIDFSFQLY DLKQQGYIER QEVKQMVVAT LAESGMNLSD EIIESIIDKT FEEADTKHDG
RIDKEEWRNL VLRHPSLLKN MTLQYLKDIT TTFPSFVFHS QVDDT
