CNBL8_ARATH
ID CNBL8_ARATH Reviewed; 214 AA.
AC Q9FUQ7; Q9SGW7;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Calcineurin B-like protein 8;
GN Name=CBL8; OrderedLocusNames=At1g64480; ORFNames=F1N19.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11115898; DOI=10.1104/pp.124.4.1844;
RA Kim K.-N., Cheong Y.H., Gupta R., Luan S.;
RT "Interaction specificity of Arabidopsis calcineurin B-like calcium sensors
RT and their target kinases.";
RL Plant Physiol. 124:1844-1853(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Albrecht V., Weinl S., Blazevic D., Kudla J.;
RT "Molecular characterization of the CBL gene family from Arabidopsis
RT thaliana.";
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP GENE FAMILY.
RX PubMed=14730064; DOI=10.1104/pp.103.033068;
RA Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., Kudla J.;
RT "Calcium sensors and their interacting protein kinases: genomics of the
RT Arabidopsis and rice CBL-CIPK signaling networks.";
RL Plant Physiol. 134:43-58(2004).
RN [6]
RP INTERACTION WITH CIPK23.
RX PubMed=16814720; DOI=10.1016/j.cell.2006.06.011;
RA Xu J., Li H.-D., Chen L.-Q., Wang Y., Liu L.-L., He L., Wu W.-H.;
RT "A protein kinase, interacting with two calcineurin B-like proteins,
RT regulates K+ transporter AKT1 in Arabidopsis.";
RL Cell 125:1347-1360(2006).
RN [7]
RP SUBCELLULAR LOCATION, DOMAIN, AND INTERACTION WITH CIPK14.
RX PubMed=19832944; DOI=10.1111/j.1365-313x.2009.04045.x;
RA Batistic O., Waadt R., Steinhorst L., Held K., Kudla J.;
RT "CBL-mediated targeting of CIPKs facilitates the decoding of calcium
RT signals emanating from distinct cellular stores.";
RL Plant J. 61:211-222(2010).
CC -!- FUNCTION: Acts as a calcium sensor. CBL proteins interact with CIPK
CC serine-threonine protein kinases. Binding of a CBL protein to the
CC regulatory NAF domain of a CIPK protein lead to the activation of the
CC kinase in a calcium-dependent manner.
CC -!- SUBUNIT: Interacts with CIPK23. Interacts with CIPK14 at the cell
CC membrane exclusively. {ECO:0000269|PubMed:16814720,
CC ECO:0000269|PubMed:19832944}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19832944}. Nucleus
CC {ECO:0000269|PubMed:19832944}. Cell membrane
CC {ECO:0000269|PubMed:19832944}.
CC -!- DOMAIN: The N-terminal 16 amino acids are sufficient for the cell
CC membrane targeting of a heterologous protein.
CC {ECO:0000269|PubMed:19832944}.
CC -!- SIMILARITY: Belongs to the calcineurin regulatory subunit family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF19691.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF290433; AAG10058.1; -; mRNA.
DR EMBL; AF411957; AAL10300.1; -; mRNA.
DR EMBL; AC009519; AAF19691.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34245.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60358.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60359.1; -; Genomic_DNA.
DR PIR; F96668; F96668.
DR RefSeq; NP_001319319.1; NM_001334162.1.
DR RefSeq; NP_001322653.1; NM_001334163.1.
DR RefSeq; NP_176629.1; NM_105123.2.
DR AlphaFoldDB; Q9FUQ7; -.
DR SMR; Q9FUQ7; -.
DR BioGRID; 27977; 3.
DR IntAct; Q9FUQ7; 1.
DR STRING; 3702.AT1G64480.1; -.
DR TCDB; 8.A.82.2.1; the calmodulin calcium binding protein (calmodulin) family.
DR PaxDb; Q9FUQ7; -.
DR PRIDE; Q9FUQ7; -.
DR EnsemblPlants; AT1G64480.1; AT1G64480.1; AT1G64480.
DR EnsemblPlants; AT1G64480.2; AT1G64480.2; AT1G64480.
DR EnsemblPlants; AT1G64480.3; AT1G64480.3; AT1G64480.
DR GeneID; 842756; -.
DR Gramene; AT1G64480.1; AT1G64480.1; AT1G64480.
DR Gramene; AT1G64480.2; AT1G64480.2; AT1G64480.
DR Gramene; AT1G64480.3; AT1G64480.3; AT1G64480.
DR KEGG; ath:AT1G64480; -.
DR Araport; AT1G64480; -.
DR TAIR; locus:2019439; AT1G64480.
DR eggNOG; KOG0034; Eukaryota.
DR HOGENOM; CLU_061288_21_0_1; -.
DR InParanoid; Q9FUQ7; -.
DR OrthoDB; 1271942at2759; -.
DR PhylomeDB; Q9FUQ7; -.
DR PRO; PR:Q9FUQ7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FUQ7; baseline and differential.
DR Genevisible; Q9FUQ7; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; ISS:TAIR.
DR GO; GO:0019900; F:kinase binding; IEA:InterPro.
DR GO; GO:0019722; P:calcium-mediated signaling; TAS:TAIR.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR045198; CNBL1-10.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR PANTHER; PTHR23056; PTHR23056; 1.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Calcium; Cell membrane; Cytoplasm; Membrane; Metal-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..214
FT /note="Calcineurin B-like protein 8"
FT /id="PRO_0000073509"
FT DOMAIN 35..70
FT /note="EF-hand 1"
FT /evidence="ECO:0000305"
FT DOMAIN 71..106
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 108..143
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 152..187
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 165
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 167
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 169
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 171
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 176
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 205
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8LAS7"
SQ SEQUENCE 214 AA; 24649 MW; BDD1CBF7C77445DD CRC64;
MLAFVKCFSL KRAKHPRGYE DPHVLASETP FTVNEIEALH DLFKKLSTSI INDGLIHKEE
FLLALFRNGS MQNLFADRVF YMFDRKRNGV IEFGEFVRSL SIFHPYTPEH EKSAFMFKLF
DLHGTGFIEP HELKKMVGAL LGETDLELSE ESIEAIVEQT MLEVDTNKDG KIDEEEWKEL
VAKNPSILKN MTLPYLKEVT LAFPSFVLDS EVED
