CNBL9_ORYSJ
ID CNBL9_ORYSJ Reviewed; 290 AA.
AC Q3HRN8; Q0JLR4;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 2.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Calcineurin B-like protein 9;
GN Name=CBL9; OrderedLocusNames=Os01g0579600, LOC_Os01g39770;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INDUCTION, AND
RP GENE FAMILY.
RC STRAIN=cv. Nipponbare; TISSUE=Seedling;
RX PubMed=18395997; DOI=10.1016/j.gene.2008.02.011;
RA Gu Z., Ma B., Jiang Y., Chen Z., Su X., Zhang H.;
RT "Expression analysis of the calcineurin B-like gene family in rice (Oryza
RT sativa L.) under environmental stresses.";
RL Gene 415:1-12(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14730064; DOI=10.1104/pp.103.033068;
RA Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., Kudla J.;
RT "Calcium sensors and their interacting protein kinases: genomics of the
RT Arabidopsis and rice CBL-CIPK signaling networks.";
RL Plant Physiol. 134:43-58(2004).
RN [7]
RP GENE FAMILY.
RX PubMed=15980189; DOI=10.1104/pp.105.062703;
RA Hwang Y.-S., Bethke P.C., Cheong Y.H., Chang H.-S., Zhu T., Jones R.L.;
RT "A gibberellin-regulated calcineurin B in rice localizes to the tonoplast
RT and is implicated in vacuole function.";
RL Plant Physiol. 138:1347-1358(2005).
CC -!- FUNCTION: Acts as a calcium sensor. CBL proteins interact with CIPK
CC serine-threonine protein kinases. Binding of a CBL protein to the
CC regulatory NAF domain of a CIPK protein lead to the activation of the
CC kinase in a calcium-dependent manner (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3HRN8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3HRN8-2; Sequence=VSP_034006, VSP_034007;
CC -!- TISSUE SPECIFICITY: Expressed in shoots and culms.
CC {ECO:0000269|PubMed:18395997}.
CC -!- INDUCTION: By abscisic acid (ABA). {ECO:0000269|PubMed:18395997}.
CC -!- SIMILARITY: Belongs to the calcineurin regulatory subunit family.
CC {ECO:0000305}.
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DR EMBL; DQ201203; ABA54184.1; -; mRNA.
DR EMBL; AP008207; BAF05314.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS72848.1; -; Genomic_DNA.
DR EMBL; AK111735; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015622218.1; XM_015766732.1. [Q3HRN8-1]
DR AlphaFoldDB; Q3HRN8; -.
DR SMR; Q3HRN8; -.
DR STRING; 4530.OS01T0579600-01; -.
DR PaxDb; Q3HRN8; -.
DR PRIDE; Q3HRN8; -.
DR EnsemblPlants; Os01t0579600-01; Os01t0579600-01; Os01g0579600. [Q3HRN8-2]
DR GeneID; 9269084; -.
DR Gramene; Os01t0579600-01; Os01t0579600-01; Os01g0579600. [Q3HRN8-2]
DR KEGG; osa:9269084; -.
DR eggNOG; KOG0034; Eukaryota.
DR InParanoid; Q3HRN8; -.
DR OMA; EKLCAVF; -.
DR OrthoDB; 1271942at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0016020; C:membrane; IEA:UniProt.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0019900; F:kinase binding; IEA:InterPro.
DR GO; GO:0019722; P:calcium-mediated signaling; IEA:InterPro.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR045198; CNBL1-10.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR PANTHER; PTHR23056; PTHR23056; 1.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..290
FT /note="Calcineurin B-like protein 9"
FT /id="PRO_0000337772"
FT DOMAIN 111..146
FT /note="EF-hand 1"
FT /evidence="ECO:0000305"
FT DOMAIN 147..182
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 184..219
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 228..263
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 241
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 243
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 245
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 247
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 252
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT SITE 220
FT /note="Involved in dimerization"
FT /evidence="ECO:0000250"
FT VAR_SEQ 191..218
FT /note="FAFRLYDLRQTGFIEREEVMQMVIAILS -> CKYTSFRHYLLKFMYRGIFT
FT IRPPFGLF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_034006"
FT VAR_SEQ 219..290
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_034007"
FT CONFLICT 120..122
FT /note="KKI -> EKT (in Ref. 1; ABA54184)"
FT /evidence="ECO:0000305"
FT CONFLICT 132
FT /note="I -> T (in Ref. 1; ABA54184)"
FT /evidence="ECO:0000305"
FT CONFLICT 137..139
FT /note="LQL -> PQP (in Ref. 1; ABA54184)"
FT /evidence="ECO:0000305"
FT CONFLICT 143
FT /note="K -> R (in Ref. 1; ABA54184)"
FT /evidence="ECO:0000305"
FT CONFLICT 148
FT /note="Q -> R (in Ref. 1; ABA54184)"
FT /evidence="ECO:0000305"
FT CONFLICT 154
FT /note="R -> W (in Ref. 1; ABA54184)"
FT /evidence="ECO:0000305"
FT CONFLICT 166
FT /note="V -> I (in Ref. 5; AK111735)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 290 AA; 32892 MW; 60803C4C249BA638 CRC64;
MESGYGFRFS DDDVESASSL TVGERLCAAF LPFVAIAEAV FFALTDCLAD LLPPSAAASR
HRRSAASSYL AAVARKWNHQ QRGRVGIGCT SLTLRQLARL ADESRCFSVN EVEALFELYK
KISCSIIDDG LIHKEELQLA LFKTPSGQNL FLDRVFDLFD EKKNGVIEFD EFIHALSVFH
PLAPLEDKIN FAFRLYDLRQ TGFIEREEVM QMVIAILSES DMKLSEELLE AIIDKTFEDA
DADRDGKINQ QEWKEFVLRH PNLLKNMTLP YLRDITTVFP SFVFNTAVED
