CNBP_DROME
ID CNBP_DROME Reviewed; 165 AA.
AC Q8T8R1; A0A0B4JD46;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=CCHC-type zinc finger nucleic acid binding protein {ECO:0000303|PubMed:24275942, ECO:0000312|FlyBase:FBgn0034802};
DE AltName: Full=CCHC-type zinc finger protein CG3800 {ECO:0000305};
GN Name=CNBP {ECO:0000303|PubMed:24275942, ECO:0000312|FlyBase:FBgn0034802};
GN ORFNames=CG3800 {ECO:0000312|FlyBase:FBgn0034802};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo, and Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:ANY27935.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Wan K., Booth B., Spirohn K., Hao T., Hu Y., Calderwood M., Hill D.,
RA Mohr S., Vidal M., Celniker S., Perrimon N.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [7]
RP FUNCTION, ASSOCIATION WITH ACTIVE POLYRIBOSOMES, AND DISRUPTION PHENOTYPE.
RX PubMed=24275942; DOI=10.4161/cc.27268;
RA Antonucci L., D'Amico D., Di Magno L., Coni S., Di Marcotullio L.,
RA Cardinali B., Gulino A., Ciapponi L., Canettieri G.;
RT "CNBP regulates wing development in Drosophila melanogaster by promoting
RT IRES-dependent translation of dMyc.";
RL Cell Cycle 13:434-439(2014).
CC -!- FUNCTION: Single-stranded DNA/RNA-binding protein with sequence
CC specificity (By similarity). Binds G-rich elements in target mRNA
CC coding sequences (By similarity). Prevents G-quadruplex structure
CC formation in vitro, suggesting a role in supporting translation by
CC resolving stable structures on mRNAs (By similarity). IRES trans-acting
CC factor (ITAF) that can promote IRES-dependent translation of Myc mRNA
CC (PubMed:24275942). Involved in the control of wing size by regulating
CC Myc levels (PubMed:24275942). {ECO:0000250|UniProtKB:P62633,
CC ECO:0000250|UniProtKB:P62634, ECO:0000269|PubMed:24275942}.
CC -!- SUBUNIT: Associates with active polyribosomes.
CC {ECO:0000269|PubMed:24275942}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P53996}. Cytoplasm
CC {ECO:0000250|UniProtKB:P62633}. Endoplasmic reticulum
CC {ECO:0000250|UniProtKB:P53996}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in lethality
CC before the pupal stage (PubMed:24275942). RNAi-mediated knockdown in
CC the wing imaginal disks results in reduction of wing size associated
CC with loss of patterning elements such as veins; this phenotype is
CC reversed by Myc overexpression (PubMed:24275942).
CC {ECO:0000269|PubMed:24275942}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE013599; AAN16117.1; -; Genomic_DNA.
DR EMBL; AE013599; ADV37238.1; -; Genomic_DNA.
DR EMBL; AY075371; AAL68216.1; -; mRNA.
DR EMBL; AY118596; AAM49965.1; -; mRNA.
DR EMBL; KX532125; ANY27935.1; -; mRNA.
DR RefSeq; NP_001188992.1; NM_001202063.2.
DR RefSeq; NP_611739.1; NM_137895.3.
DR AlphaFoldDB; Q8T8R1; -.
DR BioGRID; 63255; 19.
DR IntAct; Q8T8R1; 3.
DR STRING; 7227.FBpp0071903; -.
DR iPTMnet; Q8T8R1; -.
DR PaxDb; Q8T8R1; -.
DR PRIDE; Q8T8R1; -.
DR DNASU; 37646; -.
DR EnsemblMetazoa; FBtr0071994; FBpp0071903; FBgn0034802.
DR EnsemblMetazoa; FBtr0303464; FBpp0292516; FBgn0034802.
DR GeneID; 37646; -.
DR KEGG; dme:Dmel_CG3800; -.
DR UCSC; CG3800-RA; d. melanogaster.
DR CTD; 7555; -.
DR FlyBase; FBgn0034802; CNBP.
DR VEuPathDB; VectorBase:FBgn0034802; -.
DR eggNOG; KOG4400; Eukaryota.
DR GeneTree; ENSGT00950000183041; -.
DR HOGENOM; CLU_058879_4_0_1; -.
DR InParanoid; Q8T8R1; -.
DR OMA; SHQARDC; -.
DR OrthoDB; 1535084at2759; -.
DR PhylomeDB; Q8T8R1; -.
DR BioGRID-ORCS; 37646; 0 hits in 3 CRISPR screens.
DR ChiTaRS; CG3800; fly.
DR GenomeRNAi; 37646; -.
DR PRO; PR:Q8T8R1; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034802; Expressed in wing disc and 38 other tissues.
DR ExpressionAtlas; Q8T8R1; baseline and differential.
DR Genevisible; Q8T8R1; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:1905538; F:polysome binding; IDA:UniProtKB.
DR GO; GO:0003727; F:single-stranded RNA binding; IBA:GO_Central.
DR GO; GO:0045182; F:translation regulator activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0002192; P:IRES-dependent translational initiation of linear mRNA; IDA:UniProtKB.
DR GO; GO:2000767; P:positive regulation of cytoplasmic translation; IBA:GO_Central.
DR GO; GO:0007472; P:wing disc morphogenesis; IMP:UniProtKB.
DR InterPro; IPR001878; Znf_CCHC.
DR InterPro; IPR036875; Znf_CCHC_sf.
DR Pfam; PF00098; zf-CCHC; 6.
DR SMART; SM00343; ZnF_C2HC; 6.
DR SUPFAM; SSF57756; SSF57756; 4.
DR PROSITE; PS50158; ZF_CCHC; 6.
PE 1: Evidence at protein level;
KW Cytoplasm; Endoplasmic reticulum; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..165
FT /note="CCHC-type zinc finger nucleic acid binding protein"
FT /id="PRO_0000372843"
FT ZN_FING 5..22
FT /note="CCHC-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT ZN_FING 55..72
FT /note="CCHC-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT ZN_FING 75..92
FT /note="CCHC-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT ZN_FING 96..113
FT /note="CCHC-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT ZN_FING 123..140
FT /note="CCHC-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT ZN_FING 143..160
FT /note="CCHC-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT MOD_RES 21
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 142
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 165 AA; 17615 MW; 553E074DE830C66B CRC64;
MSMSATCYKC NRPGHFARDC SLGGGGGPGG VGGGGGGGGG GMRGNDGGGM RRNREKCYKC
NQFGHFARAC PEEAERCYRC NGIGHISKDC TQADNPTCYR CNKTGHWVRN CPEAVNERGP
TNVSCYKCNR TGHISKNCPE TSKTCYGCGK SGHLRRECDE KGGRN
