ID   CND2_ARATH              Reviewed;         671 AA.
AC   Q564K3; O80896;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Condensin complex subunit 2;
DE   AltName: Full=Chromosome-associated protein H;
DE            Short=AtCAP-H;
DE   AltName: Full=Non-SMC condensin I complex subunit H;
DE   AltName: Full=Protein EMBRYO DEFECTIVE 2795;
GN   Name=CAPH; Synonyms=EMB2795; OrderedLocusNames=At2g32590; ORFNames=T26B15;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GM
RP   DOMAIN, AND MUTAGENESIS OF 406-TRP--ARG-415.
RC   STRAIN=cv. Columbia;
RX   PubMed=15883832; DOI=10.1007/s00425-005-1546-0;
RA   Fujimoto S., Yonemura M., Matsunaga S., Nakagawa T., Uchiyama S., Fukui K.;
RT   "Characterization and dynamic analysis of Arabidopsis condensin subunits,
RT   AtCAP-H and AtCAP-H2.";
RL   Planta 222:293-300(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   IDENTIFICATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=18926486; DOI=10.1016/j.crvi.2008.07.014;
RA   Devic M.;
RT   "The importance of being essential: EMBRYO-DEFECTIVE genes in
RT   Arabidopsis.";
RL   C. R. Biol. 331:726-736(2008).
CC   -!- FUNCTION: Regulatory subunit of the condensin complex, a complex
CC       required for conversion of interphase chromatin into mitotic-like
CC       condense chromosomes. The condensin complex probably introduces
CC       positive supercoils into relaxed DNA in the presence of type I
CC       topoisomerases and converts nicked DNA into positive knotted forms in
CC       the presence of type II topoisomerases (By similarity). Essential
CC       protein. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the condensin complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15883832}.
CC       Chromosome {ECO:0000269|PubMed:15883832}. Note=Localized at mitotic
CC       chromosomes from pro-metaphase to telophase. During interphase, mainly
CC       found in cytoplasm.
CC   -!- TISSUE SPECIFICITY: Mostly expressed in flower buds and flowers, and,
CC       to a lower extent, in roots, stems, leaves and seedlings.
CC       {ECO:0000269|PubMed:15883832}.
CC   -!- DISRUPTION PHENOTYPE: Embryo development arrested at the preglobular
CC       stage. {ECO:0000269|PubMed:18926486}.
CC   -!- SIMILARITY: Belongs to the CND2 (condensin subunit 2) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC25941.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB193292; BAD95575.1; -; mRNA.
DR   EMBL; AC004681; AAC25941.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC08703.1; -; Genomic_DNA.
DR   PIR; T02558; T02558.
DR   RefSeq; NP_180818.2; NM_128818.3.
DR   AlphaFoldDB; Q564K3; -.
DR   STRING; 3702.AT2G32590.1; -.
DR   PaxDb; Q564K3; -.
DR   PRIDE; Q564K3; -.
DR   ProteomicsDB; 241240; -.
DR   EnsemblPlants; AT2G32590.1; AT2G32590.1; AT2G32590.
DR   GeneID; 817819; -.
DR   Gramene; AT2G32590.1; AT2G32590.1; AT2G32590.
DR   KEGG; ath:AT2G32590; -.
DR   Araport; AT2G32590; -.
DR   TAIR; locus:2060273; AT2G32590.
DR   eggNOG; KOG2328; Eukaryota.
DR   HOGENOM; CLU_010510_2_0_1; -.
DR   InParanoid; Q564K3; -.
DR   OMA; GREHWKV; -.
DR   OrthoDB; 702078at2759; -.
DR   PhylomeDB; Q564K3; -.
DR   PRO; PR:Q564K3; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q564K3; baseline and differential.
DR   Genevisible; Q564K3; AT.
DR   GO; GO:0000793; C:condensed chromosome; IDA:UniProtKB.
DR   GO; GO:0000796; C:condensin complex; IEA:InterPro.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007076; P:mitotic chromosome condensation; IBA:GO_Central.
DR   InterPro; IPR022816; Condensin_barren_su2.
DR   PANTHER; PTHR13108; PTHR13108; 1.
DR   Pfam; PF05786; Cnd2; 1.
DR   PIRSF; PIRSF017126; Condensin_H; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Chromosome; Cytoplasm; DNA condensation;
KW   Mitosis; Reference proteome.
FT   CHAIN           1..671
FT                   /note="Condensin complex subunit 2"
FT                   /id="PRO_0000419281"
FT   REGION          1..33
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          404..444
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          510..564
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           406..415
FT                   /note="Kleisin-gamma middle domain (GM domain) involved in
FT                   chromosome-binding"
FT   COMPBIAS        1..28
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        510..534
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        535..553
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         406..415
FT                   /note="Missing: Loss of chromosome-binding."
FT                   /evidence="ECO:0000269|PubMed:15883832"
SQ   SEQUENCE   671 AA;  75365 MW;  CEF6E2B06F430DAA CRC64;
     MDESLTPNPK QKPASTTTRI QAPTSPFFLG SNDDRLEREQ ARAARAAASR RRSVIFARGS
     QPETESDPCF DKQQILELFQ NCIKLASENK INQKNTWELN LIDHLCEIIK VEDENNTETN
     FQKASCTLEA GVKIYSMRVD SVHSEAYKVL GGITRAGHDD GGDHEDAAGA VENATNQKKQ
     PEKKISPLST LEPSFDALNV KKFDVAFAVD PLYHQTSAQF DEGGAKGLLL NNLGVYGGCQ
     VLFDSQEIPG KLVSSANKHD KSETIDLSFV KECVEQMVLN MRKKDEIVPS LRAIINQFDE
     ENQRPSDTFS CGQQTTESFD ISHGNDASYA DDDEGYENFG TSFDYEGQSG DVDENFGPNE
     AEPIYSNFHE EVEPASLQDM DSDDRLENVD DYLFLSLGIS SKQNSWAGPD HWKYRKTKGP
     DVQPASEIKS SPPAKKTRKK KQAEPELDFA KALEEEMPDI FAPPKNPKTL LLPASRTPCQ
     TKLPEDCHYQ PENLIKLFLL PNVMCLGRRR RKNSGETSRQ QPDDYEHGES WGNDNVYDDD
     DGPFDDNEND QSDAEDTNTL ISQPRQVNKI DVQYDKASKQ VDVQVLKETL WECLQESHQP
     PIQDEEHQQE PPESRSFKVL LASFPDDCQA AERTQDISPH LCFICLLHLA NEHNLSLIGS
     QNLDDLTIHL A