CNDG2_MOUSE
ID CNDG2_MOUSE Reviewed; 1138 AA.
AC Q6DFV1; E9QP71; Q6SLL0; Q922D1;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Condensin-2 complex subunit G2;
DE AltName: Full=Chromosome-associated protein G2;
DE Short=CAP-G2;
DE AltName: Full=Leucine zipper protein 5;
DE AltName: Full=More than blood protein;
DE AltName: Full=Non-SMC condensin II complex subunit G2;
GN Name=Ncapg2; Synonyms=Luzp5, Mtb;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and Czech II; TISSUE=Head, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 207-1138, FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=129/Sv;
RX PubMed=14729962; DOI=10.1128/mcb.24.3.1168-1173.2004;
RA Smith E.D., Xu Y., Tomson B.N., Leung C.G., Fujiwara Y., Orkin S.H.,
RA Crispino J.D.;
RT "More than blood, a novel gene required for mammalian postimplantation
RT development.";
RL Mol. Cell. Biol. 24:1168-1173(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Regulatory subunit of the condensin-2 complex, a complex
CC which establishes mitotic chromosome architecture and is involved in
CC physical rigidity of the chromatid axis. Is required for early
CC embryonic development and is essential for viability and expansion of
CC the inner cell mass (ICM) of the implanting blastocyst.
CC {ECO:0000269|PubMed:14729962}.
CC -!- SUBUNIT: Component of the condensin-2 complex, which contains the SMC2
CC and SMC4 heterodimer, and 3 non SMC subunits that probably regulate the
CC complex: NCAPH2, NCAPD3 and NCAPG2.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in spleen, lung and testis as well as in
CC hematopoietic cell lines. {ECO:0000269|PubMed:14729962}.
CC -!- DEVELOPMENTAL STAGE: Expressed from 7 dpc when yolk sac hematopoiesis
CC is initiated. Between 12.5 dpc and 15.5 dpc restricted to the
CC developing fetal liver and the site of definitive hematopoiesis.
CC Detected in thymus at 15.5 dpc as well as the midbrain, forebrain and
CC the olfactory epithelium. {ECO:0000269|PubMed:14729962}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH08539.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC160634; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CT030173; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC008539; AAH08539.1; ALT_SEQ; mRNA.
DR EMBL; BC076631; AAH76631.1; -; mRNA.
DR EMBL; AY455829; AAR21588.1; -; mRNA.
DR CCDS; CCDS26212.1; -.
DR RefSeq; NP_598523.3; NM_133762.3.
DR RefSeq; XP_006516412.1; XM_006516349.3.
DR RefSeq; XP_006516413.1; XM_006516350.3.
DR RefSeq; XP_006516414.1; XM_006516351.3.
DR RefSeq; XP_006516415.1; XM_006516352.3.
DR RefSeq; XP_006516416.1; XM_006516353.3.
DR RefSeq; XP_006516417.1; XM_006516354.3.
DR AlphaFoldDB; Q6DFV1; -.
DR BioGRID; 217926; 24.
DR ComplexPortal; CPX-986; Condensin II complex.
DR IntAct; Q6DFV1; 12.
DR STRING; 10090.ENSMUSP00000081889; -.
DR iPTMnet; Q6DFV1; -.
DR PhosphoSitePlus; Q6DFV1; -.
DR EPD; Q6DFV1; -.
DR MaxQB; Q6DFV1; -.
DR PaxDb; Q6DFV1; -.
DR PeptideAtlas; Q6DFV1; -.
DR PRIDE; Q6DFV1; -.
DR ProteomicsDB; 283398; -.
DR Antibodypedia; 10695; 146 antibodies from 22 providers.
DR DNASU; 76044; -.
DR Ensembl; ENSMUST00000084828; ENSMUSP00000081889; ENSMUSG00000042029.
DR GeneID; 76044; -.
DR KEGG; mmu:76044; -.
DR UCSC; uc007pht.1; mouse.
DR CTD; 54892; -.
DR MGI; MGI:1923294; Ncapg2.
DR VEuPathDB; HostDB:ENSMUSG00000042029; -.
DR eggNOG; KOG1949; Eukaryota.
DR GeneTree; ENSGT00490000043432; -.
DR HOGENOM; CLU_008117_0_0_1; -.
DR InParanoid; Q6DFV1; -.
DR OMA; SMRTLGE; -.
DR OrthoDB; 79594at2759; -.
DR PhylomeDB; Q6DFV1; -.
DR TreeFam; TF101163; -.
DR Reactome; R-MMU-2299718; Condensation of Prophase Chromosomes.
DR BioGRID-ORCS; 76044; 21 hits in 76 CRISPR screens.
DR ChiTaRS; Ncapg2; mouse.
DR PRO; PR:Q6DFV1; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q6DFV1; protein.
DR Bgee; ENSMUSG00000042029; Expressed in nasal cavity epithelium and 215 other tissues.
DR ExpressionAtlas; Q6DFV1; baseline and differential.
DR Genevisible; Q6DFV1; MM.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:ComplexPortal.
DR GO; GO:0000796; C:condensin complex; IDA:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0043425; F:bHLH transcription factor binding; IDA:MGI.
DR GO; GO:0008047; F:enzyme activator activity; IDA:MGI.
DR GO; GO:0035033; F:histone deacetylase regulator activity; IDA:MGI.
DR GO; GO:0035064; F:methylated histone binding; ISO:MGI.
DR GO; GO:0030293; F:transmembrane receptor protein tyrosine kinase inhibitor activity; IDA:MGI.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR GO; GO:0030218; P:erythrocyte differentiation; IDA:MGI.
DR GO; GO:0001833; P:inner cell mass cell proliferation; IMP:MGI.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR GO; GO:1905821; P:positive regulation of chromosome condensation; IC:ComplexPortal.
DR GO; GO:0051984; P:positive regulation of chromosome segregation; IMP:ComplexPortal.
DR GO; GO:1905820; P:positive regulation of chromosome separation; IMP:ComplexPortal.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:MGI.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR024741; Condensin2_G2.
DR Pfam; PF12422; Condensin2nSMC; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; DNA condensation; Mitosis; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..1138
FT /note="Condensin-2 complex subunit G2"
FT /id="PRO_0000255943"
FT REPEAT 459..497
FT /note="HEAT"
FT MOD_RES 30
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86XI2"
FT MOD_RES 1114
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q86XI2"
FT CONFLICT 589
FT /note="S -> P (in Ref. 3; AAR21588)"
FT /evidence="ECO:0000305"
FT CONFLICT 674
FT /note="L -> F (in Ref. 2; AAH76631)"
FT /evidence="ECO:0000305"
FT CONFLICT 723
FT /note="D -> G (in Ref. 3; AAR21588)"
FT /evidence="ECO:0000305"
FT CONFLICT 758
FT /note="Y -> C (in Ref. 3; AAR21588)"
FT /evidence="ECO:0000305"
FT CONFLICT 1101
FT /note="T -> A (in Ref. 3; AAR21588)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1138 AA; 130894 MW; B893DD92853AB1B5 CRC64;
MEKREAFIQA VSKELVEEFL QFLQLDKDSS NPFSLSELLD ELSRKQKEEL WQRLKDLLTE
TLLESPVDRW QTVEVEGADD MESEHSPKMR KSIKIICAIV TVILASVSII NEHENYGALL
ECAVILNGIL YALPESEQKL QNSIQDLCVK WWERGLPAKE DMGKTAFIML LRRSLETKSG
ADVCRLWRIH QALYCFDYDW EESREIKDML LECFINVNYI KKEEGRRFLS FLFSWNVDFI
KMIHETIKNQ LAGLQKSLMV HIAEIYFRAW KKASGKMLET IEYDCIQDFM FHGIHLLRRS
PVHSKVREVL SYFHQQKVRQ GVEEMLYRLY KPILWRGLKA RNSEVRSNAA LLFVEAFPIR
DPNFTATEMD NEIQKQFEEL YNLIEDPYPR VRSTGILGVC KISSKYWEMM PPNILVDFLK
KVTGELAFDI SSADVRCSVF KCLPIILDNK LSHPLLEQLL PTLRYSLHDN SEKVRVAFVD
LLLKIKAVRA AKFWKICPME DILVRLEMDS RPVSRRLVSL IFNSFLPVNQ PEEVWCERCV
TLIQMNRAAA RRFYQYAHEH TASTNIAKLI HVIRHCLNAC IQRTLREGSE AHKECEKENA
SVLDKTLSVN DTASMAGLLE IIVILWKNIH RSLENNKEAK IYTINKFAAV LPEYLKVFKD
ERCKIPLFML MSFLPASAVP VFSCGVISVL RNQESVTGRS YCTLLDCLCS WGQVGHVLEL
IVDWLPTVPP QAKSNLASKR KVEINDTCSV KPELALLYME YLLTHPKNRE CLLSVPQKKL
NQLLKALEGS KAELESFLQS PSGNPLNFNK ATALHAFGLY CRMSVHLQYK FCSEEKIHLS
ILDDTGSWLE NKVLPLLEDQ EEEYLKLRKD VYQQIIQTYL AVCKDVVMVG LGDPKFQMQL
LQRSFGIMKT VKGFFYVSLL LGILKEIAGN TIIHKTDSDE KVTVLFDLVQ EVFQKMLECI
ACIFRKQPEE SLPLFHSVQT PLHEFITTIQ SWHKDTAVHH AVLSTLIAAP VVEISHQLQK
VSDIEELTSP QCLHDLPPFS RCLVGVIMKS SDVVRSFVDE LKACVTSGDV EGIVCLTAVL
HIILVINKGK HISAKVKEVA TAVYRKLKTF MEITLEEDSL ERFLYESSMR TLGEFLNP
