ID   ACKMT_XENLA             Reviewed;         226 AA.
AC   Q5I047;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=ATP synthase subunit C lysine N-methyltransferase {ECO:0000305};
DE            EC=2.1.1.- {ECO:0000250|UniProtKB:Q6P4H8};
DE   AltName: Full=Protein N-lysine methyltransferase FAM173B {ECO:0000305};
GN   Name=atpsckmt; Synonyms=fam173b;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mitochondrial protein-lysine N-methyltransferase that
CC       promotes chronic pain. Involved in persistent inflammatory and
CC       neuropathic pain: methyltransferase activity in the mitochondria of
CC       sensory neurons promotes chronic pain via a pathway that depends on the
CC       production of reactive oxygen species (ROS) and on the engagement of
CC       spinal cord microglia. Protein-lysine N-methyltransferase activity is
CC       dependent on S-adenosyl-L-methionine. {ECO:0000250|UniProtKB:Q6P4H8}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC       {ECO:0000250|UniProtKB:Q6P4H8}; Single-pass membrane protein
CC       {ECO:0000255}. Note=Localizes to mitochondrial cristae.
CC       {ECO:0000250|UniProtKB:Q6P4H8}.
CC   -!- SIMILARITY: Belongs to the ANT/ATPSC lysine N-methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; BC088716; AAH88716.1; -; mRNA.
DR   RefSeq; NP_001088917.1; NM_001095448.1.
DR   AlphaFoldDB; Q5I047; -.
DR   SMR; Q5I047; -.
DR   DNASU; 496288; -.
DR   GeneID; 496288; -.
DR   KEGG; xla:496288; -.
DR   CTD; 496288; -.
DR   Xenbase; XB-GENE-6252193; atpsckmt.S.
DR   OrthoDB; 1605787at2759; -.
DR   Proteomes; UP000186698; Chromosome 6S.
DR   Bgee; 496288; Expressed in muscle tissue and 20 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0030061; C:mitochondrial crista; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0016279; F:protein-lysine N-methyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0018022; P:peptidyl-lysine methylation; ISS:UniProtKB.
DR   GO; GO:0018023; P:peptidyl-lysine trimethylation; ISS:UniProtKB.
DR   GO; GO:1905273; P:positive regulation of proton-transporting ATP synthase activity, rotational mechanism; ISS:UniProtKB.
DR   GO; GO:1904058; P:positive regulation of sensory perception of pain; ISS:UniProtKB.
DR   GO; GO:1905706; P:regulation of mitochondrial ATP synthesis coupled proton transport; ISS:UniProtKB.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR026170; FAM173A/B.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR13610; PTHR13610; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Methyltransferase; Mitochondrion; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..226
FT                   /note="ATP synthase subunit C lysine N-methyltransferase"
FT                   /id="PRO_0000321538"
FT   TRANSMEM        35..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          48..82
FT                   /note="Required for mitochondrial location"
FT                   /evidence="ECO:0000250|UniProtKB:Q6P4H8"
SQ   SEQUENCE   226 AA;  25478 MW;  91CE7FDE1C902518 CRC64;
     MSKSRKESKS LEEYSIVSST SRPKKKKWGL VATGVIGGTL VALYAVATPF VAPALRKLCL
     PYVPATTTQV KNVLKMLRSR TGIVVDIGSG DGRIVIAAAK EGFQAVGYEL NPWLVWYSRF
     RAWREGVHHH TRFYVSDLWK VSFSRYRNVV IFGVPQMMPQ LEKKLQTELQ DAARVIACRF
     PFPNWVPDHI FGEGVDTVWT YDLGAFRKVS DLKQVASKHC ILDTTV