CNG12_ARATH
ID CNG12_ARATH Reviewed; 649 AA.
AC Q8GWD2; Q9SKD5;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 21-NOV-2003, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Probable cyclic nucleotide-gated ion channel 12;
DE AltName: Full=Cyclic nucleotide- and calmodulin-regulated ion channel 12;
GN Name=CNGC12; OrderedLocusNames=At2g46450; ORFNames=F11C10.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Probable cyclic nucleotide-gated ion channel.
CC -!- SUBUNIT: Homotetramer or heterotetramer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8GWD2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8GWD2-2; Sequence=VSP_008989, VSP_008990;
CC -!- DOMAIN: The binding of calmodulin to the C-terminus might interfere
CC with cyclic nucleotide binding and thus channel activation.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD23055.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006526; AAD23055.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10697.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62322.1; -; Genomic_DNA.
DR EMBL; AK118918; BAC43501.1; -; mRNA.
DR PIR; A84903; A84903.
DR RefSeq; NP_001324486.1; NM_001337192.1. [Q8GWD2-1]
DR RefSeq; NP_850454.5; NM_180123.6. [Q8GWD2-1]
DR AlphaFoldDB; Q8GWD2; -.
DR BioGRID; 4588; 3.
DR STRING; 3702.AT2G46450.1; -.
DR PaxDb; Q8GWD2; -.
DR ProteomicsDB; 220481; -. [Q8GWD2-1]
DR EnsemblPlants; AT2G46450.1; AT2G46450.1; AT2G46450. [Q8GWD2-1]
DR EnsemblPlants; AT2G46450.4; AT2G46450.4; AT2G46450. [Q8GWD2-1]
DR GeneID; 819253; -.
DR Gramene; AT2G46450.1; AT2G46450.1; AT2G46450. [Q8GWD2-1]
DR Gramene; AT2G46450.4; AT2G46450.4; AT2G46450. [Q8GWD2-1]
DR KEGG; ath:AT2G46450; -.
DR Araport; AT2G46450; -.
DR TAIR; locus:2039114; AT2G46450.
DR eggNOG; KOG0498; Eukaryota.
DR InParanoid; Q8GWD2; -.
DR OMA; RYQYCAR; -.
DR OrthoDB; 1073751at2759; -.
DR PhylomeDB; Q8GWD2; -.
DR PRO; PR:Q8GWD2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8GWD2; baseline and differential.
DR Genevisible; Q8GWD2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
DR GO; GO:0005261; F:cation channel activity; IMP:TAIR.
DR GO; GO:0030553; F:cGMP binding; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IGI:TAIR.
DR GO; GO:0009617; P:response to bacterium; IGI:TAIR.
DR GO; GO:0009620; P:response to fungus; IMP:TAIR.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF00520; Ion_trans; 1.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50096; IQ; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calmodulin-binding; cAMP; cAMP-binding;
KW Cell membrane; cGMP; cGMP-binding; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Nucleotide-binding; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..649
FT /note="Probable cyclic nucleotide-gated ion channel 12"
FT /id="PRO_0000219340"
FT TOPO_DOM 1..43
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical; Name=H1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 65..76
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical; Name=H2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical; Name=H3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..162
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical; Name=H4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..200
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical; Name=H5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 222..329
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical; Name=H6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 351..649
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 565..594
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 545..560
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250"
FT REGION 618..649
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 436..559
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT BINDING 507
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /evidence="ECO:0000250"
FT VAR_SEQ 316..368
FT /note="SALGQNLETSNSAGEIFFAIIICVSGLLLFAVLIGNVQKYLQSSTTRVDEME
FT E -> RLIHSTVLWAKTWRQATLQGRFSLLSSYVSLVYSCLLCSLETFRSTCNHLLLE
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074"
FT /id="VSP_008989"
FT VAR_SEQ 369..649
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074"
FT /id="VSP_008990"
SQ SEQUENCE 649 AA; 74806 MW; 0737B9857B6C4EBB CRC64;
MNHRRSKFAR IDSMGVDGKL KSVRGRLKKV YGKMKTLENW RKTVLLACVV ALAIDPLFLF
IPLIDSQRFC FTFDKTLVAV VCVIRTFIDT FYVIHIIYYL ITETIAPRSQ ASLRGEIVVH
SKATLKTRLL FHFIVDIISV LPIPQVVVLT LIPLSASLVS ERILKWIILS QYVPRIIRMY
PLYKEVTRAF GTVAESKWAG AALNLFLYML HSYVFGAFWY LSSIERKSKC WRAACARTSD
CNLTVTDLLC KRAGSDNIRF LNTSCPLIDP AQITNSTDFD FGMYIDALKS GVLEVKPKDF
PRKFVYCFWW GLRNISALGQ NLETSNSAGE IFFAIIICVS GLLLFAVLIG NVQKYLQSST
TRVDEMEEKR RDTEKWMSYR VIPEYLKERI RRFEDYKWRE TKGTEEEALL RSLPKDLRLE
TKRYLYLDML KRVPWLNIMD DGWLLEAVCD RVKSVFYLAN SFIVREGHPV EEMLIVTRGK
LKSTTGSHEM GVRNNCCDLQ DGDICGELLF NGSRLPTSTR TVMTLTEVEG FILLPDDIKF
IASHLNVFQR QKLQRTFRLY SQQWRSWAAF FIQAAWRKHC KRKLSKTRDN ENIPQGTQLN
LASTLYVSRF VSKALQNRRK DTADCSSSPD MSPPVPHKPA DLEFAKAEA
