CNG16_ARATH
ID CNG16_ARATH Reviewed; 705 AA.
AC Q9SU64; Q1PEH4;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Probable cyclic nucleotide-gated ion channel 16;
DE AltName: Full=Cyclic nucleotide- and calmodulin-regulated ion channel 16;
GN Name=CNGC16; OrderedLocusNames=At3g48010; ORFNames=T17F15.120;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 86-705.
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Putative cyclic nucleotide-gated ion channel.
CC -!- SUBUNIT: Homotetramer or heterotetramer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DOMAIN: The binding of calmodulin to the C-terminus might interfere
CC with cyclic nucleotide binding and thus channel activation.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. {ECO:0000305}.
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DR EMBL; AL049658; CAB41138.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78356.1; -; Genomic_DNA.
DR EMBL; DQ446744; ABE65999.1; -; mRNA.
DR PIR; T06682; T06682.
DR RefSeq; NP_190384.1; NM_114670.2.
DR AlphaFoldDB; Q9SU64; -.
DR BioGRID; 9275; 1.
DR STRING; 3702.AT3G48010.1; -.
DR PaxDb; Q9SU64; -.
DR PRIDE; Q9SU64; -.
DR ProteomicsDB; 220290; -.
DR EnsemblPlants; AT3G48010.1; AT3G48010.1; AT3G48010.
DR GeneID; 823956; -.
DR Gramene; AT3G48010.1; AT3G48010.1; AT3G48010.
DR KEGG; ath:AT3G48010; -.
DR Araport; AT3G48010; -.
DR TAIR; locus:2097860; AT3G48010.
DR eggNOG; KOG0498; Eukaryota.
DR HOGENOM; CLU_013069_3_0_1; -.
DR InParanoid; Q9SU64; -.
DR OMA; MIWFVIP; -.
DR OrthoDB; 281394at2759; -.
DR PhylomeDB; Q9SU64; -.
DR PRO; PR:Q9SU64; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SU64; baseline and differential.
DR Genevisible; Q9SU64; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
DR GO; GO:0030553; F:cGMP binding; IEA:UniProtKB-KW.
DR GO; GO:0005216; F:ion channel activity; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
PE 2: Evidence at transcript level;
KW Calmodulin-binding; cAMP; cAMP-binding; Cell membrane; cGMP; cGMP-binding;
KW Ion channel; Ion transport; Ligand-gated ion channel; Membrane;
KW Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..705
FT /note="Probable cyclic nucleotide-gated ion channel 16"
FT /id="PRO_0000219344"
FT TOPO_DOM 1..57
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical; Name=H1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 79..91
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical; Name=H2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 113..147
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical; Name=H3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 169..180
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical; Name=H4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..222
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical; Name=H5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..353
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical; Name=H6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 375..705
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 593..622
FT /note="IQ"
FT REGION 573..588
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250"
FT REGION 636..655
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 672..705
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 638..655
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 457..580
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT BINDING 528
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /evidence="ECO:0000250"
SQ SEQUENCE 705 AA; 81955 MW; F5696626418AE93F CRC64;
MSNLHLYTSA RFRNFPTTFS LRHHHNDPNN QRRRSIFSKL RDKTLDPGGD LITRWNHIFL
ITCLLALFLD PLYFYLPIVQ AGTACMSIDV RFGIFVTCFR NLADLSFLIH ILLKFKTAFV
SKSSRVFGRG ELVMDRREIA IRYLKSEFVI DLAATLPLPQ IMIWFVIPNA GEFRYAAHQN
HTLSLIVLIQ YVPRFLVMLP LNRRIIKATG VAAKTAWSGA AYNLILYLLV SHVLGSVWYV
LSIQRQHECW RRECIKEMNA THSPSCSLLF LDCGSLHDPG RQAWMRITRV LSNCDARNDD
DQHFQFGMFG DAFTNDVTSS PFFDKYFYCL WWGLRNLSSY GQSLAASTLS SETIFSCFIC
VAGLVFFSHL IGNVQNYLQS TTARLDEWRV RRRDTEEWMR HRQLPDELQE RVRRFVQYKW
LTTRGVDEEA ILRALPLDLR RQIQRHLCLA LVRRVPFFAQ MDDQLLDAIC ERLVPSLNTK
DTYVIREGDP VNEMLFIIRG QMESSTTDGG RSGFFNSITL RPGDFCGEEL LTWALVPNIN
HNLPLSTRTV RTLSEVEAFA LRAEDLKFVA NQFRRLHSKK LQHAFRYYSH QWRAWGTCFI
QAAWRRYMKR KLAMELARQE EEDDYFYDDD GDYQFEEDMP ESNNNNGDEN SSNNQNLSAT
ILASKFAANT KRGVLGNQRG STRIDPDHPT LKMPKMFKPE DPGFF
