CNG20_ARATH
ID CNG20_ARATH Reviewed; 764 AA.
AC Q9LD37;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Probable cyclic nucleotide-gated ion channel 20, chloroplastic;
DE AltName: Full=Cyclic nucleotide-binding transporter 1;
DE Flags: Precursor;
GN Name=CNGC20; Synonyms=CNBT1; OrderedLocusNames=At3g17700; ORFNames=MKP6.28;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Columbia;
RA Wolff P., Palme K.;
RT "Two genes encoding putative cyclic nucleotide-binding ion transporters
RT from Arabidopsis thaliana.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Probable cyclic nucleotide-gated ion channel.
CC -!- SUBUNIT: Homotetramer or heterotetramer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. {ECO:0000305}.
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DR EMBL; AF148541; AAF73128.1; -; mRNA.
DR EMBL; AF148542; AAF73130.1; -; Genomic_DNA.
DR EMBL; AB022219; BAB02062.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75994.1; -; Genomic_DNA.
DR RefSeq; NP_566585.1; NM_112651.5.
DR AlphaFoldDB; Q9LD37; -.
DR BioGRID; 6370; 7.
DR IntAct; Q9LD37; 1.
DR STRING; 3702.AT3G17700.1; -.
DR TCDB; 1.A.1.5.24; the voltage-gated ion channel (vic) superfamily.
DR PaxDb; Q9LD37; -.
DR PRIDE; Q9LD37; -.
DR ProteomicsDB; 220480; -.
DR EnsemblPlants; AT3G17700.1; AT3G17700.1; AT3G17700.
DR GeneID; 821037; -.
DR Gramene; AT3G17700.1; AT3G17700.1; AT3G17700.
DR KEGG; ath:AT3G17700; -.
DR Araport; AT3G17700; -.
DR TAIR; locus:2090572; AT3G17700.
DR eggNOG; KOG0498; Eukaryota.
DR HOGENOM; CLU_013069_2_0_1; -.
DR InParanoid; Q9LD37; -.
DR OrthoDB; 212763at2759; -.
DR PhylomeDB; Q9LD37; -.
DR PRO; PR:Q9LD37; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LD37; baseline and differential.
DR Genevisible; Q9LD37; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
DR GO; GO:0030553; F:cGMP binding; IEA:UniProtKB-KW.
DR GO; GO:0005216; F:ion channel activity; IEA:InterPro.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF00520; Ion_trans; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
PE 2: Evidence at transcript level;
KW Calmodulin-binding; cAMP; cAMP-binding; cGMP; cGMP-binding; Chloroplast;
KW Ion channel; Ion transport; Ligand-gated ion channel; Membrane;
KW Nucleotide-binding; Plastid; Reference proteome; Thylakoid;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..25
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 26..764
FT /note="Probable cyclic nucleotide-gated ion channel 20,
FT chloroplastic"
FT /id="PRO_0000005556"
FT TOPO_DOM 26..204
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical; Name=H1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..242
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical; Name=H2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..295
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical; Name=H3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..329
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical; Name=H4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 351..366
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical; Name=H5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 388..488
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 489..509
FT /note="Helical; Name=H6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 510..764
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT DOMAIN 734..763
FT /note="IQ"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 713..729
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250"
FT COMPBIAS 21..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 593..710
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT BINDING 658
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /evidence="ECO:0000250"
SQ SEQUENCE 764 AA; 86950 MW; FFFA8175DB76796E CRC64;
MASHNENDDI PMLPISDPSS RTRARAFTSR SRSVSLSNPT SSIEGFDTST VVLGYTGPLR
TQRRPPLVQM SGPLTSTRKH EPLFLPHPSS DSVGVSSQPE RYPSFAALEH KNSSEDEFVL
KHANLLRSGQ LGMCNDPYCT TCPSYYNRKA AQIPTSRVSA LFDSTFHNAL YDDAKGWARR
FASSVNRYLP GIMNPHAKEV QTWTKFFALS CLLAIFIDPL FFFLIKVQEQ NKCIMIDWPM
TKAFVAVRSV TDVIFTMNIL LQFRLAYVAR ESTVVGAGQL VSHPKKIALH YLKGKFFLDL
FIVMPLPQIL ILWIIPAHLG ASGANYAKNL LRAAVLFQYI PKLYRLLPFL AGQTPTGFIF
ESAWANFVIN LLTFMLAGHV VGSCWYLFGL QRVNQCLRNA CGNFGRECQD LIDCGNGNSS
VLVRATWKDN ASANACFQED GFPYGIYLKA VNLTNHSNLF TRYSYSLFWG FQQISTLAGN
QVPSYFLGEV FFTMGIIGLG LLLFALLIGN MQNFLQALGK RNLEMTLRRR DVEQWMSHRR
LPDGIRRRVR EAERFNWAAT RGVNEELLFE NMPDDLQRDI RRHLFKFLKK VRIFSLMDEP
ILDAIRERLK QRTYIGSSTV LHRGGLVEKM VFIVRGEMES IGEDGSVLPL YEGDVCGEEL
LTWCLERSSV NPDGTRIRMP SKGLLSSRNV RCVTNVEAFS LSVADLEDVT SLFSRFLRSH
RVQGAIRYDS PYWRLRAARQ IQVAWRYRRR RLHRLCTPQS SYSL
