CNGA2_MOUSE
ID CNGA2_MOUSE Reviewed; 664 AA.
AC Q62398; Q80XH6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Cyclic nucleotide-gated olfactory channel;
DE AltName: Full=Cyclic nucleotide-gated cation channel 2;
DE AltName: Full=Cyclic nucleotide-gated channel alpha-2;
DE Short=CNG channel alpha-2;
DE Short=CNG-2;
DE Short=CNG2;
GN Name=Cnga2; Synonyms=Cncg2, Cncg4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Heart;
RX PubMed=8841933; DOI=10.1006/jmcc.1996.0136;
RA Ruiz M.L., London B., Nadal-Ginard B.;
RT "Cloning and characterization of an olfactory cyclic nucleotide-gated
RT channel expressed in mouse heart.";
RL J. Mol. Cell. Cardiol. 28:1453-1461(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Odorant signal transduction is probably mediated by a G-
CC protein coupled cascade using cAMP as second messenger. The olfactory
CC channel can be shown to be activated by cyclic nucleotides which leads
CC to a depolarization of olfactory sensory neurons.
CC -!- SUBUNIT: Heterotetramer composed of two subunits of CNGA2, one of CNGA4
CC and one of CNGB1b. The complex forms the cyclic nucleotide-gated (CNG)
CC channel of olfactory sensory neurons (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- DOMAIN: The C-terminal coiled-coil domain mediates trimerization of
CC CNGA subunits. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. CNGA2 subfamily. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-2 is the initiator.
CC {ECO:0000305}.
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DR EMBL; U49391; AAC52712.1; -; Genomic_DNA.
DR EMBL; AK132574; BAE21237.1; -; mRNA.
DR EMBL; CH466624; EDL26564.1; -; Genomic_DNA.
DR EMBL; BC048775; AAH48775.1; -; mRNA.
DR CCDS; CCDS30184.1; -.
DR RefSeq; NP_031750.2; NM_007724.3.
DR RefSeq; XP_006527824.1; XM_006527761.1.
DR AlphaFoldDB; Q62398; -.
DR SMR; Q62398; -.
DR STRING; 10090.ENSMUSP00000006020; -.
DR TCDB; 1.A.1.5.4; the voltage-gated ion channel (vic) superfamily.
DR GlyGen; Q62398; 1 site.
DR PhosphoSitePlus; Q62398; -.
DR PaxDb; Q62398; -.
DR PRIDE; Q62398; -.
DR ProteomicsDB; 285507; -.
DR Antibodypedia; 17043; 262 antibodies from 30 providers.
DR DNASU; 12789; -.
DR Ensembl; ENSMUST00000006020; ENSMUSP00000006020; ENSMUSG00000005864.
DR GeneID; 12789; -.
DR KEGG; mmu:12789; -.
DR UCSC; uc009tkk.1; mouse.
DR CTD; 1260; -.
DR MGI; MGI:108040; Cnga2.
DR VEuPathDB; HostDB:ENSMUSG00000005864; -.
DR eggNOG; KOG0500; Eukaryota.
DR GeneTree; ENSGT00940000155374; -.
DR HOGENOM; CLU_005746_12_0_1; -.
DR InParanoid; Q62398; -.
DR OMA; FPIIRQA; -.
DR OrthoDB; 1073751at2759; -.
DR PhylomeDB; Q62398; -.
DR TreeFam; TF319048; -.
DR Reactome; R-MMU-5620916; VxPx cargo-targeting to cilium.
DR BioGRID-ORCS; 12789; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q62398; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q62398; protein.
DR Bgee; ENSMUSG00000005864; Expressed in olfactory epithelium and 32 other tissues.
DR ExpressionAtlas; Q62398; baseline and differential.
DR Genevisible; Q62398; MM.
DR GO; GO:0060170; C:ciliary membrane; IDA:ARUK-UCL.
DR GO; GO:0017071; C:intracellular cyclic nucleotide activated cation channel complex; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
DR GO; GO:0030553; F:cGMP binding; IBA:GO_Central.
DR GO; GO:0005222; F:intracellular cAMP-activated cation channel activity; IBA:GO_Central.
DR GO; GO:0005223; F:intracellular cGMP-activated cation channel activity; IBA:GO_Central.
DR GO; GO:0005216; F:ion channel activity; IMP:MGI.
DR GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR GO; GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
DR GO; GO:0098655; P:cation transmembrane transport; IBA:GO_Central.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0007608; P:sensory perception of smell; IMP:MGI.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR032406; CLZ_dom.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR018488; cNMP-bd_CS.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR003938; K_chnl_volt-dep_EAG/ELK/ERG.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF16526; CLZ; 1.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR PRINTS; PR01463; EAGCHANLFMLY.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS00888; CNMP_BINDING_1; 1.
DR PROSITE; PS00889; CNMP_BINDING_2; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
PE 2: Evidence at transcript level;
KW Calmodulin-binding; cAMP; cAMP-binding; Coiled coil; Glycoprotein;
KW Ion channel; Ion transport; Ligand-gated ion channel; Membrane;
KW Nucleotide-binding; Olfaction; Reference proteome; Sensory transduction;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..664
FT /note="Cyclic nucleotide-gated olfactory channel"
FT /id="PRO_0000219313"
FT TOPO_DOM 1..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical; Name=H1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..175
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..194
FT /note="Helical; Name=H2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..218
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..238
FT /note="Helical; Name=H3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 239..276
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..299
FT /note="Helical; Name=H4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..351
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..371
FT /note="Helical; Name=H5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..455
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 456..476
FT /note="Helical; Name=H6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 477..664
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 599..642
FT /evidence="ECO:0000250"
FT COMPBIAS 1..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..40
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 464..586
FT /ligand="3',5'-cyclic AMP"
FT /ligand_id="ChEBI:CHEBI:58165"
FT /evidence="ECO:0000250"
FT BINDING 523
FT /ligand="3',5'-cyclic AMP"
FT /ligand_id="ChEBI:CHEBI:58165"
FT /evidence="ECO:0000255"
FT BINDING 538
FT /ligand="3',5'-cyclic AMP"
FT /ligand_id="ChEBI:CHEBI:58165"
FT /evidence="ECO:0000255"
FT CARBOHYD 381
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 110
FT /note="T -> P (in Ref. 1; AAC52712)"
FT /evidence="ECO:0000305"
FT CONFLICT 355
FT /note="V -> F (in Ref. 1; AAC52712)"
FT /evidence="ECO:0000305"
FT CONFLICT 540..541
FT /note="AN -> GT (in Ref. 1; AAC52712)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 664 AA; 76193 MW; 34F8DF3B372C0D9C CRC64;
MMTEKSNGVK SSPANNHNHH PPPSIKANGK DDHRAGSRPQ SVAADDDTSS ELQRLAEMDT
PRRGRGGFRR IVRLVGIIRD WANKNFREEE PRPDSFLERF RGPELQTVTT HQGDGKGDKD
GEGKGTKKKF ELFVLDPAGD WYYRWLFVIA MPVLYNWCLL VARACFSDLQ RNYFVVWLVL
DYFSDTVYIA DLIIRLRTGF LEQGLLVKDP KKLRDNYIHT LQFKLDVASI IPTDLIYFAV
GIHSPEVRFN RLLHFARMFE FFDRTETRTS YPNIFRISNL VLYILVIIHW NACIYYAISK
SIGFGVDTWV YPNITDPEYG YLAREYIYCL YWSTLTLTTI GETPPPVKDE EYLFVIFDFL
IGVLIFATIV GNVGSMISNM NATRAEFQAK IDAVKHYMQF RKVSKDMEAK VIKWFDYLWT
NKKTVDEREV LKNLPAKLRA EIAINVHLST LKKVRIFQDC EAGLLVELVL KLRPQVFSPG
DYICRKGDIG KEMYIIKEGK LAVVADDGVT QYALLSAGSC FGEISILNIK GSKMGNRRTA
NIRSLGYSDL FCLSKDDLME AVTEYPDAKK VLEERGREIL MKEGLLDENE VAASMEVDVQ
EKLEQLETNM ETLYTRFARL LAEYTGAQQK LKQRITVLET KMKQNHEDDY LSDGINTPEP
AVAE
