CNGA3_BOVIN
ID CNGA3_BOVIN Reviewed; 706 AA.
AC Q29441;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Cyclic nucleotide-gated cation channel alpha-3;
DE AltName: Full=Cone photoreceptor cGMP-gated channel subunit alpha;
DE AltName: Full=Cyclic nucleotide-gated channel alpha-3;
DE Short=CNG channel alpha-3;
DE Short=CNG-3;
DE Short=CNG3;
GN Name=CNGA3; Synonyms=CNCG3;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=7512693; DOI=10.1038/368859a0;
RA Weyand I., Godde M., Frings S., Weiner J., Mueller F., Altenhofen W.,
RA Hatt H., Kaupp U.B.;
RT "Cloning and functional expression of a cyclic-nucleotide-gated channel
RT from mammalian sperm.";
RL Nature 368:859-863(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Kidney;
RX PubMed=8170936; DOI=10.1073/pnas.91.9.3505;
RA Biel M., Zong X., Distler M., Bosse E., Klugbauer N., Murakami M.,
RA Flockerzi V., Hofmann F.;
RT "Another member of the cyclic nucleotide-gated channel family, expressed in
RT testis, kidney, and heart.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:3505-3509(1994).
CC -!- FUNCTION: Visual signal transduction is mediated by a G-protein coupled
CC cascade using cGMP as second messenger. This protein can be activated
CC by cyclic GMP which leads to an opening of the cation channel and
CC thereby causing a depolarization of cone photoreceptors. Essential for
CC the generation of light-evoked electrical responses in the red-,
CC green- and blue sensitive cones Induced a flickering channel gating,
CC weakened the outward rectification in the presence of extracellular
CC calcium, increased sensitivity for L-cis diltiazem and enhanced the
CC cAMP efficacy of the channel when coexpressed with CNGB3 (By
CC similarity). Could be responsible for cGMP-induced calcium entry in
CC cells other than sensory cells. Might be involved in chemotaxis of
CC sperm. {ECO:0000250}.
CC -!- SUBUNIT: Tetramer formed of three CNGA3 and one CNGB3 modulatory
CC subunits (By similarity). Forms functional heterooligomeric channels
CC with CNG4 in vitro. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Testis, kidney, retinal cone and heart.
CC -!- DOMAIN: The C-terminal coiled-coil domain mediates homotrimerization of
CC CNGA subunits. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. CNGA3 subfamily. {ECO:0000305}.
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DR EMBL; X89600; CAA61759.1; -; mRNA.
DR EMBL; X76485; CAA54023.1; -; mRNA.
DR PIR; A55251; A55251.
DR RefSeq; NP_776704.1; NM_174279.2.
DR AlphaFoldDB; Q29441; -.
DR SMR; Q29441; -.
DR STRING; 9913.ENSBTAP00000052464; -.
DR PaxDb; Q29441; -.
DR PRIDE; Q29441; -.
DR GeneID; 281701; -.
DR KEGG; bta:281701; -.
DR CTD; 1261; -.
DR eggNOG; KOG0500; Eukaryota.
DR HOGENOM; CLU_005746_12_0_1; -.
DR InParanoid; Q29441; -.
DR OrthoDB; 1073751at2759; -.
DR TreeFam; TF319048; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030553; F:cGMP binding; IEA:UniProtKB-KW.
DR GO; GO:0005216; F:ion channel activity; IEA:InterPro.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR032406; CLZ_dom.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR018488; cNMP-bd_CS.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF16526; CLZ; 1.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS00888; CNMP_BINDING_1; 1.
DR PROSITE; PS00889; CNMP_BINDING_2; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
PE 2: Evidence at transcript level;
KW cGMP; cGMP-binding; Coiled coil; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Nucleotide-binding; Reference proteome;
KW Sensory transduction; Transmembrane; Transmembrane helix; Transport;
KW Vision.
FT CHAIN 1..706
FT /note="Cyclic nucleotide-gated cation channel alpha-3"
FT /id="PRO_0000219316"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 322..342
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 113..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 685..706
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 645..688
FT /evidence="ECO:0000250"
FT COMPBIAS 145..177
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 501..624
FT /ligand="3',5'-cyclic GMP"
FT /ligand_id="ChEBI:CHEBI:57746"
FT BINDING 568
FT /ligand="3',5'-cyclic GMP"
FT /ligand_id="ChEBI:CHEBI:57746"
FT /evidence="ECO:0000255"
FT BINDING 583
FT /ligand="3',5'-cyclic GMP"
FT /ligand_id="ChEBI:CHEBI:57746"
FT /evidence="ECO:0000255"
SQ SEQUENCE 706 AA; 81132 MW; F4990DCD29B56239 CRC64;
MAKISTQYSH PTRTHPSVRT MDRDLDCIEN GLSRTHLPCE ETSSELQEGI AMETRGLAES
RQSSFTSQGP TRLSRLIISL RAWSARHLHQ EDQRPDSFLE RFRGAELQEV SSRESHVQFN
VGSQEPPDRG RSAWPLARNN TNTCNNSEKD DKAKKEEKEK KEEKKENPKK EEKKKDSVVM
DPSSNMYYHW LTVIAVPVFY NWCLLVCRAC FDELQSEHLM LWLVLDYSAD ILYGMDMLVR
ARTGFLEQGL MVMDASRLWK HYTQTLHFKL DVLSLVPTDL AYFKLGMNYP ELRFNRLLKL
ARLFEFFDRT ETRTNYPNMF RIGNLVLYIL IIIHWNACIY FAISKFIGFG TDSWVYPNVS
NPEYGRLSRK YIYSLYWSTL TLTTIGETPP PVKDEEYLFV VIDFLVGVLI FATIVGNVGS
MISNMNASRA EFQAKIDSIK QYMQFRKVTK DLETRVIRWF DYLWANKKTV DEKEVLKSLP
DKLKAEIAIN VHLDTLRKVR IFQDCEAGLL VELVLKLRPA VFSPGDYICK KGDIGREMYI
IKEGKLAVVA EDGITQFVVL GDGSYFGEIS ILNIKGSKSG NRRTANIRSI GYSDLFCLSK
DDLMEALTEY PEAKKALEEK GRQILMKDNL IDEELAKAGA DPKDIEEKVE HLETSLDSLQ
TRFARLLAEY NATQMKVKQR LSQLESQVKM GLPPDGDAPQ TEASQP
