CNGA3_MOUSE
ID CNGA3_MOUSE Reviewed; 631 AA.
AC Q9JJZ8; Q8CFV6; Q9WV01;
DT 04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Cyclic nucleotide-gated cation channel alpha-3;
DE AltName: Full=Cone photoreceptor cGMP-gated channel subunit alpha;
DE AltName: Full=Cyclic nucleotide-gated channel alpha-3;
DE Short=CNG channel alpha-3;
DE Short=CNG-3;
DE Short=CNG3;
GN Name=Cnga3; Synonyms=Cng3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Retina;
RX PubMed=10813773;
RX DOI=10.1002/(sici)1096-9861(20000522)421:1<80::aid-cne5>3.0.co;2-o;
RA Hirano A.A., Hack I., Waessle H., Duvoisin R.M.;
RT "Cloning and immunocytochemical localization of a cyclic nucleotide-gated
RT channel alpha-subunit to all cone photoreceptors in the mouse retina.";
RL J. Comp. Neurol. 421:80-94(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-631.
RC STRAIN=129/Sv;
RX PubMed=10377453; DOI=10.1073/pnas.96.13.7553;
RA Biel M., Seeliger M., Pfeifer A., Kohler K., Gerstner A., Ludwig A.,
RA Jaissle G., Fauser S., Zrenner E., Hofmann F.;
RT "Selective loss of cone function in mice lacking the cyclic nucleotide-
RT gated channel CNG3.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:7553-7557(1999).
RN [4]
RP FUNCTION, AND SUBUNIT.
RX PubMed=10662822; DOI=10.1523/jneurosci.20-04-01324.2000;
RA Gerstner A., Zong X., Hofmann F., Biel M.;
RT "Molecular cloning and functional characterization of a new modulatory
RT cyclic nucleotide-gated channel subunit from mouse retina.";
RL J. Neurosci. 20:1324-1332(2000).
CC -!- FUNCTION: Visual signal transduction is mediated by a G-protein coupled
CC cascade using cGMP as second messenger. This protein can be activated
CC by cyclic GMP which leads to an opening of the cation channel and
CC thereby causing a depolarization of cone photoreceptors. Essential for
CC the generation of light-evoked electrical responses in the red-,
CC green- and blue sensitive cones (By similarity). Induced a flickering
CC channel gating, weakened the outward rectification in the presence of
CC extracellular calcium, increased sensitivity for L-cis diltiazem and
CC enhanced the cAMP efficacy of the channel when coexpressed with CNGB3.
CC {ECO:0000250, ECO:0000269|PubMed:10662822}.
CC -!- SUBUNIT: Tetramer formed of three CNGA3 and one CNGB3 modulatory
CC subunits. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Prominently expressed in retina.
CC -!- DOMAIN: The C-terminal coiled-coil domain mediates homotrimerization of
CC CNGA subunits. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. CNGA3 subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ243933; CAB89685.1; -; mRNA.
DR EMBL; BC035272; AAH35272.1; -; mRNA.
DR EMBL; BC049145; AAH49145.1; -; mRNA.
DR EMBL; AJ238239; CAB42891.1; -; Genomic_DNA.
DR EMBL; AJ238240; CAB42891.1; JOINED; Genomic_DNA.
DR EMBL; AJ238241; CAB42891.1; JOINED; Genomic_DNA.
DR CCDS; CCDS14890.1; -.
DR RefSeq; NP_001268939.1; NM_001282010.1.
DR RefSeq; NP_034048.1; NM_009918.2.
DR RefSeq; XP_017169234.1; XM_017313745.1.
DR AlphaFoldDB; Q9JJZ8; -.
DR SMR; Q9JJZ8; -.
DR BioGRID; 198785; 19.
DR STRING; 10090.ENSMUSP00000027288; -.
DR iPTMnet; Q9JJZ8; -.
DR PhosphoSitePlus; Q9JJZ8; -.
DR PaxDb; Q9JJZ8; -.
DR PRIDE; Q9JJZ8; -.
DR ABCD; Q9JJZ8; 1 sequenced antibody.
DR Antibodypedia; 47515; 130 antibodies from 22 providers.
DR DNASU; 12790; -.
DR Ensembl; ENSMUST00000027288; ENSMUSP00000027288; ENSMUSG00000026114.
DR Ensembl; ENSMUST00000194195; ENSMUSP00000142075; ENSMUSG00000026114.
DR GeneID; 12790; -.
DR KEGG; mmu:12790; -.
DR UCSC; uc007ari.2; mouse.
DR CTD; 1261; -.
DR MGI; MGI:1341818; Cnga3.
DR VEuPathDB; HostDB:ENSMUSG00000026114; -.
DR eggNOG; KOG0500; Eukaryota.
DR GeneTree; ENSGT00940000158737; -.
DR HOGENOM; CLU_005746_12_0_1; -.
DR InParanoid; Q9JJZ8; -.
DR OMA; YIGINNP; -.
DR OrthoDB; 1073751at2759; -.
DR PhylomeDB; Q9JJZ8; -.
DR TreeFam; TF319048; -.
DR BioGRID-ORCS; 12790; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q9JJZ8; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9JJZ8; protein.
DR Bgee; ENSMUSG00000026114; Expressed in retinal neural layer and 36 other tissues.
DR ExpressionAtlas; Q9JJZ8; baseline and differential.
DR GO; GO:0043194; C:axon initial segment; ISO:MGI.
DR GO; GO:0120199; C:cone photoreceptor outer segment; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0030425; C:dendrite; ISO:MGI.
DR GO; GO:0097386; C:glial cell projection; ISO:MGI.
DR GO; GO:0017071; C:intracellular cyclic nucleotide activated cation channel complex; IBA:GO_Central.
DR GO; GO:0043025; C:neuronal cell body; ISO:MGI.
DR GO; GO:0043204; C:perikaryon; ISO:MGI.
DR GO; GO:0001750; C:photoreceptor outer segment; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:1902495; C:transmembrane transporter complex; ISO:MGI.
DR GO; GO:0030553; F:cGMP binding; ISO:MGI.
DR GO; GO:0005222; F:intracellular cAMP-activated cation channel activity; IBA:GO_Central.
DR GO; GO:0005223; F:intracellular cGMP-activated cation channel activity; ISO:MGI.
DR GO; GO:0005221; F:intracellular cyclic nucleotide activated cation channel activity; IPI:MGI.
DR GO; GO:0008022; F:protein C-terminus binding; ISO:MGI.
DR GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR GO; GO:0098655; P:cation transmembrane transport; IBA:GO_Central.
DR GO; GO:0006812; P:cation transport; ISO:MGI.
DR GO; GO:0098659; P:inorganic cation import across plasma membrane; ISO:MGI.
DR GO; GO:0034220; P:ion transmembrane transport; ISO:MGI.
DR GO; GO:0051591; P:response to cAMP; ISO:MGI.
DR GO; GO:0032026; P:response to magnesium ion; ISO:MGI.
DR GO; GO:0046549; P:retinal cone cell development; IMP:MGI.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR032406; CLZ_dom.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR018488; cNMP-bd_CS.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF16526; CLZ; 1.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS00888; CNMP_BINDING_1; 1.
DR PROSITE; PS00889; CNMP_BINDING_2; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
PE 1: Evidence at protein level;
KW cGMP; cGMP-binding; Coiled coil; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Nucleotide-binding; Reference proteome;
KW Sensory transduction; Transmembrane; Transmembrane helix; Transport;
KW Vision.
FT CHAIN 1..631
FT /note="Cyclic nucleotide-gated cation channel alpha-3"
FT /id="PRO_0000219318"
FT TRANSMEM 112..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 71..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 567..610
FT /evidence="ECO:0000250"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 423..546
FT /ligand="3',5'-cyclic GMP"
FT /ligand_id="ChEBI:CHEBI:57746"
FT BINDING 490
FT /ligand="3',5'-cyclic GMP"
FT /ligand_id="ChEBI:CHEBI:57746"
FT /evidence="ECO:0000255"
FT BINDING 505
FT /ligand="3',5'-cyclic GMP"
FT /ligand_id="ChEBI:CHEBI:57746"
FT /evidence="ECO:0000255"
FT CONFLICT 110
FT /note="Y -> C (in Ref. 1; CAB89685)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="L -> V (in Ref. 3; CAB42891)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 631 AA; 72702 MW; 4FAD66E3A8A3FE6C CRC64;
MAKVNTQCSQ PSPTQLSIKN ADRDLDHVEN GLGRVSRLII SIRAWASRHL HDEDQTPDSF
LDRFHGSELK EVSTRESNAQ PNPGEQKPPD GGEGRKEEPI VVDPSSNIYY RWLTAIALPV
FYNWCLLVCR ACFDELQSEH LTLWLVLDYS ADVLYVLDML VRARTGFLEQ GLMVRDTKRL
WKHYTKTLHF KLDILSLIPT DLAYLKLGVN YPELRFNRLL KFSRLFEFFD RTETRTNYPN
VFRIGNLVLY TLIIIHWNAC IYFAISKFIG FGTDSWVYPN TSKPEYARLS RKYIYSLYWS
TLTLTTIGET PPPVKDEEYL FVVIDFLVGI LIFATIVGNV GSMISNMNAP RVEFQAKIDS
VKQYMQFRKV TKDLETRVIR WFDYLWANRK TVDEKEVLKN LPDKLKAEIA INVHLDTLKK
VRIFQDCEAG LLVELVLKLR PTVFSPGDYI CKKGDIGREM YIIKEGKLAV VADDGVTQFV
VLSDGSYFGE ISILNIKGSK SGNRRTANIR SIGYSDLFCL SKDDLMEALT EYPDAKRALE
EKGRQILMKD NLIDEDLVAA RVDTRDVEEK VEYLESSLDI LQTRFARLLA EYSASQMKLK
QRLTRLESQM NRRCCGFSPD RENSEDASKT D
