CNGC4_ARATH
ID CNGC4_ARATH Reviewed; 694 AA.
AC Q94AS9; Q9XFS2;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 21-NOV-2003, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Cyclic nucleotide-gated ion channel 4;
DE Short=AtCNGC4;
DE AltName: Full=Cyclic nucleotide- and calmodulin-regulated ion channel 4;
DE Short=AtHLM1;
GN Name=CNGC4; Synonyms=HLM1; OrderedLocusNames=At5g54250; ORFNames=MDK4.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=10341447; DOI=10.1046/j.1365-313x.1999.00422.x;
RA Koehler C., Merkle T., Neuhaus G.;
RT "Characterisation of a novel gene family of putative cyclic nucleotide- and
RT calmodulin-regulated ion channels in Arabidopsis thaliana.";
RL Plant J. 18:97-104(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=12566578; DOI=10.1105/tpc.006999;
RA Balague C., Lin B., Alcon C., Flottes G., Malmstroem S., Koehler C.,
RA Neuhaus G., Pelletier G., Gaymard F., Roby D.;
RT "HLM1, an essential signaling component in the hypersensitive response, is
RT a member of the cyclic nucleotide-gated channel ion channel family.";
RL Plant Cell 15:365-379(2003).
CC -!- FUNCTION: Acts as cyclic nucleotide-gated ion channel. Permeable to
CC potassium and sodium in a cyclic nucleotide-dependent fashion (cAMP or
CC cGMP). Might constitute a common downstream component of the signaling
CC pathways leading to hypersensitive response (HR).
CC {ECO:0000269|PubMed:12566578}.
CC -!- SUBUNIT: Homotetramer or heterotetramer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q94AS9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q94AS9-2; Sequence=VSP_008985, VSP_008986;
CC -!- INDUCTION: Induced by both ethylene and methyl jasmonate treatments, or
CC after pathogen attack.
CC -!- DOMAIN: The binding of calmodulin to the C-terminus might interfere
CC with cyclic nucleotide binding and thus channel activation.
CC {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Loss-of-function mutation results in the loss of
CC the hypersensitive response leading to broad spectrum disease
CC resistance, and displays a lesion-mimic phenotype.
CC {ECO:0000269|PubMed:12566578}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. {ECO:0000305}.
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DR EMBL; Y17912; CAB40129.1; -; mRNA.
DR EMBL; AB010695; BAB10748.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96473.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96474.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70509.1; -; Genomic_DNA.
DR EMBL; AY045822; AAK76496.1; -; mRNA.
DR EMBL; AY057691; AAL15321.1; -; mRNA.
DR EMBL; AY133734; AAM91668.1; -; mRNA.
DR PIR; T52574; T52574.
DR RefSeq; NP_001332114.1; NM_001345080.1. [Q94AS9-1]
DR RefSeq; NP_200236.1; NM_124805.3. [Q94AS9-1]
DR RefSeq; NP_851188.1; NM_180857.2. [Q94AS9-1]
DR AlphaFoldDB; Q94AS9; -.
DR STRING; 3702.AT5G54250.2; -.
DR TCDB; 1.A.1.5.7; the voltage-gated ion channel (vic) superfamily.
DR iPTMnet; Q94AS9; -.
DR PaxDb; Q94AS9; -.
DR PRIDE; Q94AS9; -.
DR ProteomicsDB; 220482; -. [Q94AS9-1]
DR EnsemblPlants; AT5G54250.1; AT5G54250.1; AT5G54250. [Q94AS9-1]
DR EnsemblPlants; AT5G54250.2; AT5G54250.2; AT5G54250. [Q94AS9-1]
DR EnsemblPlants; AT5G54250.4; AT5G54250.4; AT5G54250. [Q94AS9-1]
DR GeneID; 835513; -.
DR Gramene; AT5G54250.1; AT5G54250.1; AT5G54250. [Q94AS9-1]
DR Gramene; AT5G54250.2; AT5G54250.2; AT5G54250. [Q94AS9-1]
DR Gramene; AT5G54250.4; AT5G54250.4; AT5G54250. [Q94AS9-1]
DR KEGG; ath:AT5G54250; -.
DR Araport; AT5G54250; -.
DR TAIR; locus:2162605; AT5G54250.
DR eggNOG; KOG0498; Eukaryota.
DR HOGENOM; CLU_013069_3_1_1; -.
DR InParanoid; Q94AS9; -.
DR OMA; KGPNPIT; -.
DR PhylomeDB; Q94AS9; -.
DR PRO; PR:Q94AS9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q94AS9; baseline and differential.
DR Genevisible; Q94AS9; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0030553; F:cGMP binding; IEA:UniProtKB-KW.
DR GO; GO:0005216; F:ion channel activity; IEA:InterPro.
DR GO; GO:0009626; P:plant-type hypersensitive response; IMP:TAIR.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF00520; Ion_trans; 1.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calmodulin-binding; cAMP; cAMP-binding;
KW Cell membrane; cGMP; cGMP-binding; Hypersensitive response; Ion channel;
KW Ion transport; Ligand-gated ion channel; Membrane; Nucleotide-binding;
KW Plant defense; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..694
FT /note="Cyclic nucleotide-gated ion channel 4"
FT /id="PRO_0000219332"
FT TOPO_DOM 1..92
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical; Name=H1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 114..126
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical; Name=H2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..187
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical; Name=H3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..216
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical; Name=H4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 238..251
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical; Name=H5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..392
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical; Name=H6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 414..694
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 631..660
FT /note="IQ"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 610..626
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250"
FT COMPBIAS 1..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..54
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 496..626
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT BINDING 565
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /evidence="ECO:0000250"
FT VAR_SEQ 274..293
FT /note="AAGACWYLLGVQRSAKCLKE -> VSYLINCFVLLNILVNTKFQ (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_008985"
FT VAR_SEQ 294..694
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_008986"
SQ SEQUENCE 694 AA; 80081 MW; E3F843AE1B0F1EA0 CRC64;
MATEQEFTRA SRFSRDSSSV GYYSEEDNTE EEDEEEEEME EIEEEEEEEE EEDPRIGLTC
GGRRNGSSNN NKWMMLGRIL DPRSKWVREW NKVFLLVCAT GLFVDPLFLY TLSVSDTCMC
LLVDGWLALT VTALRSMTDL LHLWNIWIQF KIARRWPYPG GDSDGDTNKG GGTRGSTRVA
PPYVKKNGFF FDLFVILPLP QVVLWVVIPS LLKRGSVTLV VSVLLVTFLF QYLPKIYHSI
RHLRRNATLS GYIFGTVWWG IALNMIAYFV AAHAAGACWY LLGVQRSAKC LKEQCENTIG
CDLRMLSCKE PVYYGTTVMV LDRARLAWAQ NHQARSVCLD INTNYTYGAY QWTIQLVSSE
SRLEKILFPI FWGLMTLSTF GNLESTTEWS EVVFNIIVLT SGLLLVTMLI GNIKVFLHAT
TSKKQAMHLK MRNIEWWMKK RHLPIGFRQR VRNYERQRWA AMRGVDECEM VQNLPEGLRR
DIKYHLCLDL VRQVPLFQHM DDLVLENICD RVKSLIFTKG ETIQKEGDAV QRMLFVVRGH
LQSSQLLRDG VKSCCMLGPG NFSGDELLSW CLRRPFVERL PPSSSTLVTL ETTEAFGLDA
EDVKYVTQHF RYTFVNEKVK RSARYYSPGW RTWAAVAVQL AWRRYKHRLT LTSLSFIRPR
RPLSRCASLG EDKLRLYAAI LTSPKPNPDD FDDY