CNGC5_ARATH
ID CNGC5_ARATH Reviewed; 717 AA.
AC Q8RWS9; Q9XFS3;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Probable cyclic nucleotide-gated ion channel 5;
DE Short=AtCNGC5;
DE AltName: Full=Cyclic nucleotide- and calmodulin-regulated ion channel 5;
GN Name=CNGC5; OrderedLocusNames=At5g57940; ORFNames=MTI20.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=10341447; DOI=10.1046/j.1365-313x.1999.00422.x;
RA Koehler C., Merkle T., Neuhaus G.;
RT "Characterisation of a novel gene family of putative cyclic nucleotide- and
RT calmodulin-regulated ion channels in Arabidopsis thaliana.";
RL Plant J. 18:97-104(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Probable cyclic nucleotide-gated ion channel.
CC -!- SUBUNIT: Homotetramer or heterotetramer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8RWS9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8RWS9-2; Sequence=VSP_008987;
CC -!- DOMAIN: The binding of calmodulin to the C-terminus might interfere
CC with cyclic nucleotide binding and thus channel activation.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. {ECO:0000305}.
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DR EMBL; Y17913; CAB40130.1; -; mRNA.
DR EMBL; AB013396; BAB08864.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96974.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96975.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96976.1; -; Genomic_DNA.
DR EMBL; AY091133; AAM14082.1; -; mRNA.
DR EMBL; AY114053; AAM45101.1; -; mRNA.
DR PIR; T52573; T52573.
DR RefSeq; NP_200602.2; NM_125179.5. [Q8RWS9-1]
DR RefSeq; NP_851209.1; NM_180878.2. [Q8RWS9-1]
DR RefSeq; NP_974953.1; NM_203224.1. [Q8RWS9-2]
DR AlphaFoldDB; Q8RWS9; -.
DR BioGRID; 21149; 31.
DR IntAct; Q8RWS9; 28.
DR STRING; 3702.AT5G57940.1; -.
DR PaxDb; Q8RWS9; -.
DR PRIDE; Q8RWS9; -.
DR ProteomicsDB; 241242; -. [Q8RWS9-1]
DR EnsemblPlants; AT5G57940.1; AT5G57940.1; AT5G57940. [Q8RWS9-1]
DR EnsemblPlants; AT5G57940.2; AT5G57940.2; AT5G57940. [Q8RWS9-1]
DR EnsemblPlants; AT5G57940.3; AT5G57940.3; AT5G57940. [Q8RWS9-2]
DR GeneID; 835905; -.
DR Gramene; AT5G57940.1; AT5G57940.1; AT5G57940. [Q8RWS9-1]
DR Gramene; AT5G57940.2; AT5G57940.2; AT5G57940. [Q8RWS9-1]
DR Gramene; AT5G57940.3; AT5G57940.3; AT5G57940. [Q8RWS9-2]
DR KEGG; ath:AT5G57940; -.
DR Araport; AT5G57940; -.
DR TAIR; locus:2174448; AT5G57940.
DR eggNOG; KOG0498; Eukaryota.
DR InParanoid; Q8RWS9; -.
DR OMA; FYLLHMA; -.
DR PhylomeDB; Q8RWS9; -.
DR PRO; PR:Q8RWS9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8RWS9; baseline and differential.
DR Genevisible; Q8RWS9; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0035618; C:root hair; IDA:TAIR.
DR GO; GO:0005262; F:calcium channel activity; IDA:TAIR.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
DR GO; GO:0030553; F:cGMP binding; IEA:UniProtKB-KW.
DR GO; GO:0005223; F:intracellular cGMP-activated cation channel activity; IDA:TAIR.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50096; IQ; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calmodulin-binding; cAMP; cAMP-binding;
KW Cell membrane; cGMP; cGMP-binding; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Nucleotide-binding; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..717
FT /note="Probable cyclic nucleotide-gated ion channel 5"
FT /id="PRO_0000219333"
FT TOPO_DOM 1..102
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical; Name=H1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 124..136
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical; Name=H2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..190
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical; Name=H3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..224
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical; Name=H4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..265
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical; Name=H5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..391
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical; Name=H6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 413..717
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 634..663
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 614..629
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250"
FT BINDING 498..628
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT BINDING 569
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /evidence="ECO:0000250"
FT VAR_SEQ 10..16
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10341447"
FT /id="VSP_008987"
SQ SEQUENCE 717 AA; 81969 MW; 444FF45621AE6BDF CRC64;
MAGKRENFVR VDDLDSRLPS SSVAFQQNYA SNFSGQLHPI HASNETSRSF KKGIQKGSKG
LKSIGRSLGF GVYRAVFPED LKVSEKKIFD PQDKFLLYCN KLFVASCILS VFVDPFFFYL
PVINAESKCL GIDRKLAITA STLRTFIDVF YLAHMALQLR TAYIAPSSRV FGRGELVIDP
AQIAKRYLQR WFIIDFLSVL PLPQIVVWRF LQSSNGSDVL ATKQALLFIV LVQYIPRFLR
VLPLTSELKR TAGVFAETAW AGAAYYLLLY MLASHIVGAF WYLLALERND ACWQEACIDA
GNCSTDFLYC GNQNMDGYAV WNRAKESVLK SKCRADLDDN NPPFDFGIYT QALSSGIVSS
QNFIVKYCYC LWWGLQNLST LGQGLETSTY PMEIIFSISL AISGLILFAL LIGNMQTYLQ
SLTIRLEEMR VKRRDSEQWM HHRMLPQDLR ERVRRYDQYK WLETRGVDEE YLVQNLPKDL
RRDIKRHLCL ALVRRVPLFK SMDDKLLDAI CMRLKPCLFT ESTYLVREGD PVDEMLFIIR
GRLESVTTDG GRSGFFNRSL LKEGEFCGEE LLTWALDPKS GVNLPSSTRT VKALTEVEAF
ALTSEELKFV ASQFRRLHSR QVQHTFRFYS HQWRTWAACF IQAAWRRYCK RKKMEEAEAE
AAAVSSSTAG PSYSIGAAFL ATKFAANALR TIHRNRNTKI RDLVKLQKPP EPDFTAD
