CNGC6_ARATH
ID CNGC6_ARATH Reviewed; 747 AA.
AC O82226; Q9XFS4;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Probable cyclic nucleotide-gated ion channel 6;
DE Short=AtCNGC6;
DE AltName: Full=Cyclic nucleotide- and calmodulin-regulated ion channel 6;
GN Name=CNGC6; OrderedLocusNames=At2g23980; ORFNames=T29E15.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=10341447; DOI=10.1046/j.1365-313x.1999.00422.x;
RA Koehler C., Merkle T., Neuhaus G.;
RT "Characterisation of a novel gene family of putative cyclic nucleotide- and
RT calmodulin-regulated ion channels in Arabidopsis thaliana.";
RL Plant J. 18:97-104(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
RN [5]
RP STRUCTURE BY NMR OF 509-632.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the cnmp-binding domain from Arabidopsis thaliana
RT cyclic nucleotide-regulated ion channel.";
RL Submitted (NOV-2004) to the PDB data bank.
CC -!- FUNCTION: Probable cyclic nucleotide-gated ion channel.
CC -!- SUBUNIT: Homotetramer or heterotetramer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DOMAIN: The binding of calmodulin to the C-terminus might interfere
CC with cyclic nucleotide binding and thus channel activation.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. {ECO:0000305}.
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DR EMBL; Y17914; CAB40131.1; -; mRNA.
DR EMBL; AC005170; AAC63666.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC07510.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61675.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61676.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61680.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61682.1; -; Genomic_DNA.
DR PIR; B84631; B84631.
DR PIR; T52572; T52572.
DR RefSeq; NP_001318278.1; NM_001335878.1.
DR RefSeq; NP_001323879.1; NM_001335882.1.
DR RefSeq; NP_001323883.1; NM_001335886.1.
DR RefSeq; NP_001323885.1; NM_001335881.1.
DR RefSeq; NP_565560.1; NM_127960.3.
DR PDB; 1WGP; NMR; -; A=509-632.
DR PDBsum; 1WGP; -.
DR AlphaFoldDB; O82226; -.
DR SMR; O82226; -.
DR BioGRID; 2283; 17.
DR IntAct; O82226; 16.
DR STRING; 3702.AT2G23980.1; -.
DR TCDB; 1.A.1.5.14; the voltage-gated ion channel (vic) superfamily.
DR iPTMnet; O82226; -.
DR PaxDb; O82226; -.
DR PRIDE; O82226; -.
DR ProteomicsDB; 241243; -.
DR EnsemblPlants; AT2G23980.1; AT2G23980.1; AT2G23980.
DR EnsemblPlants; AT2G23980.10; AT2G23980.10; AT2G23980.
DR EnsemblPlants; AT2G23980.4; AT2G23980.4; AT2G23980.
DR EnsemblPlants; AT2G23980.5; AT2G23980.5; AT2G23980.
DR EnsemblPlants; AT2G23980.9; AT2G23980.9; AT2G23980.
DR GeneID; 816931; -.
DR Gramene; AT2G23980.1; AT2G23980.1; AT2G23980.
DR Gramene; AT2G23980.10; AT2G23980.10; AT2G23980.
DR Gramene; AT2G23980.4; AT2G23980.4; AT2G23980.
DR Gramene; AT2G23980.5; AT2G23980.5; AT2G23980.
DR Gramene; AT2G23980.9; AT2G23980.9; AT2G23980.
DR KEGG; ath:AT2G23980; -.
DR Araport; AT2G23980; -.
DR TAIR; locus:2061401; AT2G23980.
DR eggNOG; KOG0498; Eukaryota.
DR HOGENOM; CLU_013069_3_0_1; -.
DR InParanoid; O82226; -.
DR PhylomeDB; O82226; -.
DR EvolutionaryTrace; O82226; -.
DR PRO; PR:O82226; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82226; baseline and differential.
DR Genevisible; O82226; AT.
DR GO; GO:0071944; C:cell periphery; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0035618; C:root hair; IDA:TAIR.
DR GO; GO:0005262; F:calcium channel activity; IDA:TAIR.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
DR GO; GO:0030553; F:cGMP binding; IEA:UniProtKB-KW.
DR GO; GO:0005223; F:intracellular cGMP-activated cation channel activity; IDA:TAIR.
DR GO; GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
DR GO; GO:0009408; P:response to heat; IDA:TAIR.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR003938; K_chnl_volt-dep_EAG/ELK/ERG.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF00520; Ion_trans; 1.
DR PRINTS; PR01463; EAGCHANLFMLY.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50096; IQ; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calmodulin-binding; cAMP; cAMP-binding; Cell membrane; cGMP;
KW cGMP-binding; Ion channel; Ion transport; Ligand-gated ion channel;
KW Membrane; Nucleotide-binding; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..747
FT /note="Probable cyclic nucleotide-gated ion channel 6"
FT /id="PRO_0000219334"
FT TOPO_DOM 1..117
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical; Name=H1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..150
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical; Name=H2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..205
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical; Name=H3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 227..239
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical; Name=H4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 261..280
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical; Name=H5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 302..407
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical; Name=H6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 429..747
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 650..679
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 630..645
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250"
FT BINDING 514..638
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT BINDING 585
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /evidence="ECO:0000250"
FT HELIX 514..516
FT /evidence="ECO:0007829|PDB:1WGP"
FT HELIX 520..529
FT /evidence="ECO:0007829|PDB:1WGP"
FT STRAND 539..542
FT /evidence="ECO:0007829|PDB:1WGP"
FT STRAND 549..556
FT /evidence="ECO:0007829|PDB:1WGP"
FT STRAND 560..562
FT /evidence="ECO:0007829|PDB:1WGP"
FT STRAND 568..572
FT /evidence="ECO:0007829|PDB:1WGP"
FT STRAND 574..576
FT /evidence="ECO:0007829|PDB:1WGP"
FT STRAND 582..584
FT /evidence="ECO:0007829|PDB:1WGP"
FT HELIX 586..592
FT /evidence="ECO:0007829|PDB:1WGP"
FT STRAND 598..600
FT /evidence="ECO:0007829|PDB:1WGP"
FT STRAND 604..611
FT /evidence="ECO:0007829|PDB:1WGP"
FT STRAND 613..619
FT /evidence="ECO:0007829|PDB:1WGP"
FT HELIX 620..630
FT /evidence="ECO:0007829|PDB:1WGP"
SQ SEQUENCE 747 AA; 85441 MW; 601234797E44700F CRC64;
MFDTCGPKGV KSQVISGQRE NFVRLDSMDS RYSQSSETGL NKCTLNIQGG PKRFAQGSKA
SSGSFKKGFR KGSEGLWSIG RSIGLGVSRA VFPEDLEVSE KKIFDPQDKF LLLCNKLFVA
SCILAVSVDP LFLYLPFIND KAKCVGIDRK LAIIVTTIRT VIDSFYLFHM ALRFRTAYVA
PSSRVFGRGE LVIDPAQIAK RYLQQYFIID LLSVLPVPQI IVWRFLYTSR GANVLATKQA
LRYIVLVQYI PRFLRMYPLS SELKRTAGVF AETAWAGAAY YLLLYMLASH IVGALWYLLA
LERNNDCWSK ACHNNQNCTR NFLFCGNQNM KGYAAWDNIK VSYLQLKCPV NVPEDEEPPF
DFGIYLRALS SGIVSSKNFV SKYFFCLWWG LQNLSTLGQG LETSTYPGEV IFSITLAIAG
LLLFALLIGN MQTYLQSLTI RLEEMRVKRR DSEQWMHHRM LPPELRERVR RYDQYKWLET
RGVDEENLVQ NLPKDLRRDI KRHLCLALVR RVPLFENMDE RLLDAICERL KPCLFTEKSY
LVREGDPVNE MLFIIRGRLE SVTTDGGRSG FYNRSLLKEG DFCGDELLTW ALDPKSGSNL
PSSTRTVKAL TEVEAFALIA DELKFVASQF RRLHSRQVQH TFRFYSQQWR TWAACFMQAA
WRRYIKRKKL EQLRKEEEEE EAAAASVIAG GSPYSIRATF LASKFAANAL RSVHKNRTAK
STLLLSSTKE LVKFQKPPEP DFSAEDH
