CNGC8_ARATH
ID CNGC8_ARATH Reviewed; 753 AA.
AC Q9FXH6;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 21-NOV-2003, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Putative cyclic nucleotide-gated ion channel 8;
DE AltName: Full=Cyclic nucleotide- and calmodulin-regulated ion channel 8;
GN Name=CNGC8; OrderedLocusNames=At1g19780; ORFNames=F14P1.12, F6F9.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Putative cyclic nucleotide-gated ion channel.
CC -!- SUBUNIT: Homotetramer or heterotetramer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DOMAIN: The binding of calmodulin to the C-terminus might interfere
CC with cyclic nucleotide binding and thus channel activation.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG12561.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007797; AAG12561.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC024609; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE29896.1; -; Genomic_DNA.
DR PIR; H86330; H86330.
DR RefSeq; NP_173408.2; NM_101834.4.
DR AlphaFoldDB; Q9FXH6; -.
DR STRING; 3702.AT1G19780.1; -.
DR iPTMnet; Q9FXH6; -.
DR PaxDb; Q9FXH6; -.
DR PRIDE; Q9FXH6; -.
DR ProteomicsDB; 220483; -.
DR EnsemblPlants; AT1G19780.1; AT1G19780.1; AT1G19780.
DR GeneID; 838566; -.
DR Gramene; AT1G19780.1; AT1G19780.1; AT1G19780.
DR KEGG; ath:AT1G19780; -.
DR Araport; AT1G19780; -.
DR TAIR; locus:2013139; AT1G19780.
DR eggNOG; KOG0498; Eukaryota.
DR HOGENOM; CLU_013069_3_0_1; -.
DR InParanoid; Q9FXH6; -.
DR OMA; EACTEND; -.
DR OrthoDB; 281394at2759; -.
DR PhylomeDB; Q9FXH6; -.
DR PRO; PR:Q9FXH6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FXH6; baseline and differential.
DR Genevisible; Q9FXH6; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
DR GO; GO:0030553; F:cGMP binding; IEA:UniProtKB-KW.
DR GO; GO:0005216; F:ion channel activity; IEA:InterPro.
DR GO; GO:0009860; P:pollen tube growth; IGI:TAIR.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50096; IQ; 1.
PE 3: Inferred from homology;
KW Calmodulin-binding; cAMP; cAMP-binding; Cell membrane; cGMP; cGMP-binding;
KW Ion channel; Ion transport; Ligand-gated ion channel; Membrane;
KW Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..753
FT /note="Putative cyclic nucleotide-gated ion channel 8"
FT /id="PRO_0000219336"
FT TOPO_DOM 1..111
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical; Name=H1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..145
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical; Name=H2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..199
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical; Name=H3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..233
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical; Name=H4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..274
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..295
FT /note="Helical; Name=H5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 296..402
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical; Name=H6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 424..753
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 644..673
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 624..639
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250"
FT REGION 731..753
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 508..638
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT BINDING 579
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /evidence="ECO:0000250"
SQ SEQUENCE 753 AA; 85983 MW; 5AB2A74E25BC7CE4 CRC64;
MYKSQYISGH REKFVRLDDT DSRVSMSSNA TGMKKRSCFG LFNVTSRGGG KTKNTSKSFR
EGVKIGSEGL KTIGKSFTSG VTRAVFPEDL RVSEKKIFDP QDKTLLLWNR MFVISCILAV
SVDPLFFYLP IVDNSKNCIG IDSKLAVTTT TLRTIIDVFY LTRMALQFRT AYIAPSSRVF
GRGELVIDPA KIAERYLTRY FIVDFLAVLP LPQIAVWKFL HGSKGTDVLP TKQALLHIVI
TQYIPRFVRF IPLTSELKKT AGAFAEGAWA GAAYYLLWYM LASHITGAFW YMLSVERNDT
CLRSACKVQP DPKVCVQILY CGSKLMSSRE TDWIKSVPDL FKNNCSAKSD ESKFNYGIYS
QAVSSGIVSS TTFFSKFCYC LWWGLQNLST LGQGLQTSTY PGEVLFSIAI AVAGLLLFAL
LIGNMQTYLQ SLTVRLEEMR IKRRDSEQWM HHRSLPQNLR ERVRRYDQYK WLETRGVDEE
NIVQSLPKDL RRDIKRHLCL NLVRRVPLFA NMDERLLDAI CERLKPSLYT ESTYIVREGD
PVNEMLFIIR GRLESVTTDG GRSGFFNRGL LKEGDFCGEE LLTWALDPKA GSNLPSSTRT
VKALTEVEAF ALEAEELKFV ASQFRRLHSR QVQQTFRFYS QQWRTWAACF IQAAWRRHLR
RKIAELRRKE EEEEEMDYED DEYYDDNMGG MVTRSDSSVG SSSTLRSTVF ASRFAANALK
GHKLRVTESS KSLMNLTKPS EPDFEALDTD DLN
