CNGC9_ARATH
ID CNGC9_ARATH Reviewed; 733 AA.
AC Q9M0A4;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Putative cyclic nucleotide-gated ion channel 9;
DE AltName: Full=Cyclic nucleotide- and calmodulin-regulated ion channel 9;
GN Name=CNGC9; OrderedLocusNames=At4g30560; ORFNames=F17I23.100;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Putative cyclic nucleotide-gated ion channel.
CC -!- SUBUNIT: Homotetramer or heterotetramer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9M0A4-1; Sequence=Displayed;
CC -!- DOMAIN: The binding of calmodulin to the C-terminus might interfere
CC with cyclic nucleotide binding and thus channel activation.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC
CC 1.A.1.5) family. {ECO:0000305}.
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DR EMBL; AL161577; CAB79774.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85780.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM68123.1; -; Genomic_DNA.
DR PIR; E85357; E85357.
DR RefSeq; NP_001329900.1; NM_001342013.1. [Q9M0A4-1]
DR RefSeq; NP_194785.1; NM_119202.4. [Q9M0A4-1]
DR AlphaFoldDB; Q9M0A4; -.
DR BioGRID; 14466; 25.
DR IntAct; Q9M0A4; 23.
DR STRING; 3702.AT4G30560.1; -.
DR iPTMnet; Q9M0A4; -.
DR PaxDb; Q9M0A4; -.
DR PRIDE; Q9M0A4; -.
DR ProteomicsDB; 220395; -. [Q9M0A4-1]
DR EnsemblPlants; AT4G30560.1; AT4G30560.1; AT4G30560. [Q9M0A4-1]
DR EnsemblPlants; AT4G30560.4; AT4G30560.4; AT4G30560. [Q9M0A4-1]
DR GeneID; 829179; -.
DR Gramene; AT4G30560.1; AT4G30560.1; AT4G30560. [Q9M0A4-1]
DR Gramene; AT4G30560.4; AT4G30560.4; AT4G30560. [Q9M0A4-1]
DR KEGG; ath:AT4G30560; -.
DR Araport; AT4G30560; -.
DR TAIR; locus:2118836; AT4G30560.
DR eggNOG; KOG0498; Eukaryota.
DR InParanoid; Q9M0A4; -.
DR OrthoDB; 281394at2759; -.
DR PhylomeDB; Q9M0A4; -.
DR PRO; PR:Q9M0A4; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9M0A4; baseline and differential.
DR Genevisible; Q9M0A4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005262; F:calcium channel activity; IDA:TAIR.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
DR GO; GO:0030553; F:cGMP binding; IEA:UniProtKB-KW.
DR GO; GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR003938; K_chnl_volt-dep_EAG/ELK/ERG.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR PRINTS; PR01463; EAGCHANLFMLY.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50096; IQ; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Calmodulin-binding; cAMP; cAMP-binding;
KW Cell membrane; cGMP; cGMP-binding; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Nucleotide-binding; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..733
FT /note="Putative cyclic nucleotide-gated ion channel 9"
FT /id="PRO_0000219337"
FT TOPO_DOM 1..117
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical; Name=H1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..151
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical; Name=H2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..207
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical; Name=H3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..239
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical; Name=H4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 261..280
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical; Name=H5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 302..406
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical; Name=H6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 428..733
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 649..678
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 629..644
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250"
FT BINDING 513..637
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT BINDING 584
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /evidence="ECO:0000250"
SQ SEQUENCE 733 AA; 83624 MW; 522D3A190B0423DE CRC64;
MLDCGKKAVK SQVISGRLEK FVRLDSMDSR YSQTSDTGLN RCTLNLQGPT RGGGAQGNNV
SSGSFKKGFR KGSKGLWSIG RSIGLGVSRA VFPEDLKVSE KKIFDPQDKF LLLCNKLFVT
SCILAVSVDP LFLYLPFVKD NEKCIGIDRK LAIIATTLRT VIDAFYLFHM ALRFRTAFVA
PSSRVFGRGE LVIDPAQIAK RYLQQYFIID FLSVLPLPQI VVWRFLYISK GASVLATKRA
LRSIILVQYI PRFIRLYPLS SELKRTAGVF AETAWAGAAY YLLLYMLASH IVGAIWYLLA
LERYNGCWTK VCSNSSLDCH RNFLFCGNEK MDGYAAWTTI KDSVLQLNCP VNTTDNPPFD
FGIYLRALSS GIVSSKSFVS KYFFCLWWGL QNLSTLGQGL ETSTYPGEVI FSIALAIAGL
LLFALLIGNM QTYLQSLTIR LEEMRVKRRD SEQWMHHRML PPELRERVRR YDQYKWLETR
GVDEENLVQN LPKDLRRDIK RHLCLALVRR VPLFENMDER LLDAICERLK PCLYTESSYL
VREGDPVNEM LFIIRGRLES VTTDGGRSGF FNRSLLKEGD FCGEELLTWA LDPKSGSNLP
SSTRTAKALT EVEAFALIAD ELKFVASQFR RLHSRQVQHT FRFYSQQWRT WAAIFIQAAW
RRYVKKKKLE QLRKEEEEGE GSVTSIRATF LASKFAANAL RKVHKNRIEA KSTIELVKYQ
KPSEPDFSAD DTS
