CNIPF_HUMAN
ID CNIPF_HUMAN Reviewed; 899 AA.
AC G9CGD6; G9CGD3; G9CGD4;
DT 20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT 22-FEB-2012, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=CNK3/IPCEF1 fusion protein {ECO:0000303|PubMed:22085542};
GN Name=CNK3/IPCEF1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS CNK3-IPCEF1-1; CNK3-IPCEF1-2 AND
RP CNK3-IPCEF1-3), AND FUNCTION.
RX PubMed=22085542; DOI=10.1016/j.yexcr.2011.10.018;
RA Attar M.A., Salem J.C., Pursel H.S., Santy L.C.;
RT "CNK3 and IPCEF1 produce a single protein that is required for HGF
RT dependent Arf6 activation and migration.";
RL Exp. Cell Res. 318:228-237(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-383, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-873, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Required for hepatocyte growth factor (HGF)-dependent
CC activation of Arf6 and HGF-stimulated cell migration.
CC {ECO:0000269|PubMed:22085542}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=CNK3-IPCEF1-1; Synonyms=CNK3/IPCEF1 Long-1;
CC IsoId=G9CGD6-1; Sequence=Displayed;
CC Name=CNK3-IPCEF1-2; Synonyms=CNK3/IPCEF1 Long-2;
CC IsoId=G9CGD6-2; Sequence=VSP_059280;
CC Name=CNK3-IPCEF1-3; Synonyms=CNK3/IPCEF1 Short;
CC IsoId=G9CGD6-3; Sequence=VSP_059281;
CC Name=CNK3;
CC IsoId=Q6P9H4-1; Sequence=External;
CC Name=IPCEF1-1;
CC IsoId=Q8WWN9-1; Sequence=External;
CC Name=IPCEF1-2;
CC IsoId=Q8WWN9-2; Sequence=External;
CC -!- MISCELLANEOUS: [Isoform CNK3-IPCEF1-1]: Based on a naturally occurring
CC readthrough transcript which produces an CNK3/IPCEF1 fusion protein.
CC {ECO:0000269|PubMed:22085542}.
CC -!- MISCELLANEOUS: [Isoform CNK3-IPCEF1-2]: Based on a naturally occurring
CC readthrough transcript which produces an CNK3/IPCEF1 fusion protein.
CC {ECO:0000269|PubMed:22085542}.
CC -!- MISCELLANEOUS: [Isoform CNK3-IPCEF1-3]: Based on a naturally occurring
CC readthrough transcript which produces an CNK3/IPCEF1 fusion protein.
CC Major isoform found in CaCo2-cells. {ECO:0000269|PubMed:22085542}.
CC -!- SIMILARITY: Belongs to the CNKSR family. {ECO:0000305}.
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DR EMBL; JF308526; AEA29623.1; -; mRNA.
DR EMBL; JF308523; AEA29620.1; -; mRNA.
DR EMBL; JF308524; AEA29621.1; -; mRNA.
DR EMBL; AL033376; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL132774; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL357075; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL445220; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL590401; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KF510943; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; G9CGD6; -.
DR SMR; G9CGD6; -.
DR IntAct; G9CGD6; 2.
DR iPTMnet; G9CGD6; -.
DR PhosphoSitePlus; G9CGD6; -.
DR jPOST; G9CGD6; -.
DR MassIVE; G9CGD6; -.
DR PeptideAtlas; G9CGD6; -.
DR PRIDE; G9CGD6; -.
DR UCSC; uc021zhc.2; human. [G9CGD6-1]
DR HPA; ENSG00000288520; Low tissue specificity.
DR neXtProt; NX_G9CGD6; -.
DR VEuPathDB; HostDB:ENSG00000288520; -.
DR GeneTree; ENSGT00940000154428; -.
DR HOGENOM; CLU_013414_0_0_1; -.
DR OMA; ACHPKVM; -.
DR Pharos; G9CGD6; Tbio.
DR Proteomes; UP000005640; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:2001114; P:positive regulation of cellular response to hepatocyte growth factor stimulus; IMP:UniProtKB.
DR GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR Gene3D; 1.10.150.50; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR InterPro; IPR010599; CNK2/3_dom.
DR InterPro; IPR017874; CRIC_domain.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR Pfam; PF10534; CRIC_ras_sig; 1.
DR Pfam; PF06663; DUF1170; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00536; SAM_1; 1.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00454; SAM; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR SUPFAM; SSF50156; SSF50156; 1.
DR PROSITE; PS51290; CRIC; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50105; SAM_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..899
FT /note="CNK3/IPCEF1 fusion protein"
FT /id="PRO_0000442776"
FT DOMAIN 7..72
FT /note="SAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00184"
FT DOMAIN 80..174
FT /note="CRIC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00621"
FT DOMAIN 211..293
FT /note="PDZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 332..457
FT /note="DUF1170"
FT /evidence="ECO:0000255"
FT DOMAIN 503..602
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 309..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 347..390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 605..687
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 735..770
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 851..899
FT /note="Required for interaction with CYTH2"
FT /evidence="ECO:0000250|UniProtKB:Q8WWN9"
FT REGION 868..899
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 631..687
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 735..749
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 383
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 873
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT VAR_SEQ 474
FT /note="L -> LQ (in isoform CNK3-IPCEF1-2)"
FT /id="VSP_059280"
FT VAR_SEQ 475..543
FT /note="Missing (in isoform CNK3-IPCEF1-3)"
FT /id="VSP_059281"
FT CONFLICT 647
FT /note="S -> L (in Ref. 1; AEA29621)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 899 AA; 100365 MW; AE0CF42C290EADD5 CRC64;
MEPVTKWSPK QVVDWTRGLD DCLQQYVHKF EREKINGEQL LQISHQDLEE LGVTRIGHQE
LVLEAVDLLC ALNYGLETDN MKNLVLKLRA SSHNLQNYIS SRRKSPAYDG NTSRKAPNEF
LTSVVELIGA AKALLAWLDR APFTGITDFS VTKNKIIQLC LDLTTTVQKD CFVAEMEDKV
LTVVKVLNGI CDKTIRSTTD PVMSQCACLE EVHLPNIKPG EGLGMYIKST YDGLHVITGT
TENSPADRSQ KIHAGDEVIQ VNQQTVVGWQ LKNLVKKLRE NPTGVVLLLK KRPTGSFNFT
PAPLKNLRWK PPLVQTSPPP ATTQSPESTM DTSLKKEKSA ILDLYIPPPP AVPYSPRDEN
GSFVYGGSSK CKQPLPGPKG SESPNSFLDQ ESRRRRFTIA DSDQLPGYSV ETNILPTKMR
EKTPSYGKPR PLSMPADGNW MGIVDPFARP RGHGRKAFVS TKMTSYMAID GSALVPLRQK
PRRKTQGFLT MSRRRISCKD LGHADCQGWL YKKKEKGSFL SNKWKKFWVI LKGSSLYWYS
NQMAEKADGF VNLPDFTVER ASECKKKHAF KISHPQIKTF YFAAENVQEM NVWLNKLGSA
VIHQESTTKD EECYSESEQE DPEIAAETPP PPHASQTQSL TAQQASSSSP SLSGTSYSFS
SLENTVKTPS SFPSSLSKER QSLPDTVNSL SAAEDEGQPI TFAVQVHSPV PSEAGIHKAL
ENSFVTSESG FLNSLSSDDT SSLSSNHDHL TVPDKPAGSK IMDKEETKVS EDDEMEKLYK
SLEQASLSPL GDRRPSTKKE LRKSFVKRCK NPSINEKLHK IRTLNSTLKC KEHDLAMINQ
LLDDPKLTAR KYREWKVMNT LLIQDIYQQQ RASPAPDDTD DTPQELKKSP SSPSVENSI
