ID   CNJC_TETTH              Reviewed;         310 AA.
AC   P16925;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Conjugation stage-specific protein;
GN   Name=CNJC;
OS   Tetrahymena thermophila.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC   Tetrahymena.
OX   NCBI_TaxID=5911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=B;
RX   PubMed=2112240; DOI=10.1093/nar/18.10.2953;
RA   Martindale D.W.;
RT   "A conjugation-specific gene (cnjC) from Tetrahymena encodes a protein
RT   homologous to yeast RNA polymerase subunits (RPB3, RPC40) and similar to a
RT   portion of the prokaryotic RNA polymerase alpha subunit (rpoA).";
RL   Nucleic Acids Res. 18:2953-2960(1990).
CC   -!- FUNCTION: May be a stage-specific RNA polymerase subunit.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DEVELOPMENTAL STAGE: Sexual stage (conjugation) of life cycle.
CC   -!- SIMILARITY: Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase
CC       subunit family. {ECO:0000305}.
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DR   EMBL; X62317; CAA44194.1; -; mRNA.
DR   PIR; S12807; S12807.
DR   AlphaFoldDB; P16925; -.
DR   SMR; P16925; -.
DR   OMA; ENTSPFH; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.170.120.12; -; 1.
DR   Gene3D; 3.30.1360.10; -; 1.
DR   HAMAP; MF_00320; RNApol_arch_Rpo3; 1.
DR   InterPro; IPR001514; DNA-dir_RNA_pol_30-40kDasu_CS.
DR   InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR   InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR022842; RNAP_Rpo3/Rpb3/RPAC1.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   Pfam; PF01000; RNA_pol_A_bac; 1.
DR   Pfam; PF01193; RNA_pol_L; 1.
DR   SMART; SM00662; RPOLD; 1.
DR   SUPFAM; SSF55257; SSF55257; 1.
DR   SUPFAM; SSF56553; SSF56553; 1.
DR   PROSITE; PS00446; RNA_POL_D_30KD; 1.
PE   2: Evidence at transcript level;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Nucleus;
KW   Transcription; Transferase.
FT   CHAIN           1..310
FT                   /note="Conjugation stage-specific protein"
FT                   /id="PRO_0000132749"
SQ   SEQUENCE   310 AA;  36042 MW;  A4965DADCA65C071 CRC64;
     MEGIKLTLTN LEEFKCEFTL ENCDISLANS LRRILLAEIE TIAIHNVKVY ENTSSLPDEY
     IAHRLGLIPL NSAKVDDYNY IWNCNCQGEC DRCTIHFDLR VSNFTNEVLE VTSLDLKQRQ
     DHTDEDAVRP IKVYSILRDE YGNKREVGIP IIKLSKGQQI HFECEAQKGI GKMHSKWNPV
     CISTFSVEPE IIFDQEIQNL DIEQKKALVN ACPTKVFGIN PHTNTLEVQD HMKCMYCEEC
     VYKCAEDFQK PKLIKIDHKK DKFFFKVETT GVMKPVEVLR RGFSTLRQKL DFMREQIQEN
     SNNQFEYGNE