CNN1_MOUSE
ID CNN1_MOUSE Reviewed; 297 AA.
AC Q08091;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Calponin-1;
DE AltName: Full=Basic calponin;
DE AltName: Full=Calponin H1, smooth muscle;
GN Name=Cnn1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Smooth muscle;
RX PubMed=8370452; DOI=10.1016/0014-5793(93)80909-e;
RA Strasser P., Gimona M., Moessler H., Herzog M., Small J.V.;
RT "Mammalian calponin. Identification and expression of genetic variants.";
RL FEBS Lett. 330:13-18(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RA Gao J.M., Hwang J.M., Resek M.E., Jin J.P.;
RL Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv; TISSUE=Liver;
RA Miano J.M., Olson E.N.;
RL Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=8550594; DOI=10.1074/jbc.271.1.395;
RA Samaha F.F., Ip H.S., Morrisey E.E., Seltzer J., Tang Z., Solway J.,
RA Parmacek M.S.;
RT "Developmental pattern of expression and genomic organization of the
RT calponin-h1 gene. A contractile smooth muscle cell marker.";
RL J. Biol. Chem. 271:395-403(1996).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Thin filament-associated protein that is implicated in the
CC regulation and modulation of smooth muscle contraction. It is capable
CC of binding to actin, calmodulin and tropomyosin. The interaction of
CC calponin with actin inhibits the actomyosin Mg-ATPase activity.
CC -!- SUBUNIT: Part of cGMP kinase signaling complex at least composed of
CC ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and
CC ITPR1. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Alpha;
CC IsoId=Q08091-1; Sequence=Displayed;
CC Name=Beta;
CC IsoId=Q08091-2; Sequence=VSP_000755;
CC -!- TISSUE SPECIFICITY: Smooth muscle, and tissues containing significant
CC amounts of smooth muscle.
CC -!- SIMILARITY: Belongs to the calponin family. {ECO:0000305}.
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DR EMBL; Z19542; CAA79602.1; -; mRNA.
DR EMBL; L49022; AAB01453.1; -; Genomic_DNA.
DR EMBL; L49022; AAB01452.1; -; Genomic_DNA.
DR EMBL; U28932; AAC52448.1; -; Genomic_DNA.
DR EMBL; U40351; AAC52353.1; -; Genomic_DNA.
DR EMBL; U38930; AAC52353.1; JOINED; Genomic_DNA.
DR EMBL; U40348; AAC52353.1; JOINED; Genomic_DNA.
DR EMBL; U40349; AAC52353.1; JOINED; Genomic_DNA.
DR EMBL; U40350; AAC52353.1; JOINED; Genomic_DNA.
DR CCDS; CCDS22921.1; -. [Q08091-1]
DR PIR; S36145; S31486.
DR RefSeq; NP_034052.3; NM_009922.4. [Q08091-1]
DR AlphaFoldDB; Q08091; -.
DR SMR; Q08091; -.
DR STRING; 10090.ENSMUSP00000001384; -.
DR iPTMnet; Q08091; -.
DR PhosphoSitePlus; Q08091; -.
DR CPTAC; non-CPTAC-3970; -.
DR MaxQB; Q08091; -.
DR PaxDb; Q08091; -.
DR PeptideAtlas; Q08091; -.
DR PRIDE; Q08091; -.
DR ProteomicsDB; 279120; -. [Q08091-1]
DR ProteomicsDB; 279121; -. [Q08091-2]
DR Antibodypedia; 1965; 1097 antibodies from 42 providers.
DR DNASU; 12797; -.
DR Ensembl; ENSMUST00000001384; ENSMUSP00000001384; ENSMUSG00000001349. [Q08091-1]
DR GeneID; 12797; -.
DR KEGG; mmu:12797; -.
DR UCSC; uc009onv.2; mouse. [Q08091-1]
DR CTD; 1264; -.
DR MGI; MGI:104979; Cnn1.
DR VEuPathDB; HostDB:ENSMUSG00000001349; -.
DR eggNOG; KOG2046; Eukaryota.
DR GeneTree; ENSGT00940000159680; -.
DR HOGENOM; CLU_055232_0_0_1; -.
DR InParanoid; Q08091; -.
DR OMA; GEPTHNH; -.
DR PhylomeDB; Q08091; -.
DR TreeFam; TF313921; -.
DR BioGRID-ORCS; 12797; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q08091; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q08091; protein.
DR Bgee; ENSMUSG00000001349; Expressed in ascending aorta and 118 other tissues.
DR ExpressionAtlas; Q08091; baseline and differential.
DR Genevisible; Q08091; MM.
DR GO; GO:0005856; C:cytoskeleton; ISO:MGI.
DR GO; GO:0003779; F:actin binding; ISO:MGI.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0031032; P:actomyosin structure organization; IEA:InterPro.
DR GO; GO:1904706; P:negative regulation of vascular associated smooth muscle cell proliferation; ISO:MGI.
DR CDD; cd00014; CH; 1.
DR Gene3D; 1.10.418.10; -; 1.
DR InterPro; IPR001997; Calponin/LIMCH1.
DR InterPro; IPR000557; Calponin_repeat.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR003096; SM22_calponin.
DR Pfam; PF00402; Calponin; 3.
DR Pfam; PF00307; CH; 1.
DR PRINTS; PR00889; CALPONIN.
DR PRINTS; PR00888; SM22CALPONIN.
DR SMART; SM00033; CH; 1.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS01052; CALPONIN_1; 3.
DR PROSITE; PS51122; CALPONIN_2; 3.
DR PROSITE; PS50021; CH; 1.
PE 1: Evidence at protein level;
KW Actin-binding; Alternative splicing; Calmodulin-binding; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..297
FT /note="Calponin-1"
FT /id="PRO_0000204768"
FT DOMAIN 28..131
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT REPEAT 164..189
FT /note="Calponin-like 1"
FT REPEAT 204..229
FT /note="Calponin-like 2"
FT REPEAT 243..268
FT /note="Calponin-like 3"
FT MOD_RES 170
FT /note="Phosphothreonine; by ROCK2"
FT /evidence="ECO:0000250"
FT MOD_RES 175
FT /note="Phosphoserine; by ROCK2"
FT /evidence="ECO:0000250"
FT MOD_RES 180
FT /note="Phosphothreonine; by ROCK2"
FT /evidence="ECO:0000250"
FT MOD_RES 184
FT /note="Phosphothreonine; by ROCK2"
FT /evidence="ECO:0000250"
FT MOD_RES 259
FT /note="Phosphothreonine; by ROCK2"
FT /evidence="ECO:0000250"
FT VAR_SEQ 217..256
FT /note="Missing (in isoform Beta)"
FT /evidence="ECO:0000305"
FT /id="VSP_000755"
FT CONFLICT 35
FT /note="R -> E (in Ref. 2; AAB01453/AAB01452)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 297 AA; 33356 MW; 0D1F1F31899700ED CRC64;
MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF MDGLKDGIIL
CEFINKLQPG SVKKVNESTQ NWHQLENIGN FIKAITKYGV KPHDIFEAND LFENTNHTQV
QSTLLALASM AKTKGNKVNV GVKYAEKQER RFEPEKLREG RNIIGLQMGT NKFASQQGMT
AYGTRRHLYD PKLGTDQPLD QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT
LNVSLQMGSN KGASQRGMTV YGLPRQVYDP KYCLNPEYPE LSEPTHNHHP HNYYNSA
