CNNM1_CAEEL
ID CNNM1_CAEEL Reviewed; 811 AA.
AC A3QM97;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Metal transporter cnnm-1 {ECO:0000305};
DE AltName: Full=CNNM family homolog 1 {ECO:0000312|WormBase:C52D10.12};
DE Flags: Precursor;
GN Name=cnnm-1 {ECO:0000303|PubMed:27564576, ECO:0000312|WormBase:C52D10.12};
GN ORFNames=C52D10.12 {ECO:0000312|WormBase:C52D10.12};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=27564576; DOI=10.1371/journal.pgen.1006276;
RA Ishii T., Funato Y., Hashizume O., Yamazaki D., Hirata Y., Nishiwaki K.,
RA Kono N., Arai H., Miki H.;
RT "Mg2+ extrusion from intestinal epithelia by CNNM proteins is essential for
RT gonadogenesis via AMPK-TORC1 signaling in Caenorhabditis elegans.";
RL PLoS Genet. 12:E1006276-E1006276(2016).
CC -!- FUNCTION: Probable metal transporter. Probably acts redundantly with
CC the other metal transport proteins cnnm-2, cnnm-3, cnnm-4 and cnnm-5 to
CC regulate Mg(2+) homeostasis. Promotes postembryonic gonad development
CC by regulating Mg(2+) levels, probably via AMPK signaling.
CC {ECO:0000269|PubMed:27564576}.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane
CC {ECO:0000269|PubMed:27564576}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the intestine and in neurons,
CC but it is also expressed in a variety of tissues including the pharynx,
CC hypodermis, rectum and in muscles. {ECO:0000269|PubMed:27564576}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Double knockout with cnnm-3
CC results in increased levels of intestinal Mg(2+) and reduced levels in
CC other tissues. This Mg(2+) deficiency in tissues leads to a reduced
CC lifespan, 100% sterility, and smaller animals that exhibit a
CC developmental delay with defective gonad development and which
CC therefore do not produce oocytes or form vulva. In addition, the gonad
CC development defect in the cnnm-1 and cnnm-3 double knockout is rescued
CC when the AMPK alpha subunit aak-2 is also knocked out. Quintuple
CC knockout with cnnm-2, cnnm-3, cnnm-4 and cnnm-5 results in a reduced
CC lifespan and 100% sterility. {ECO:0000269|PubMed:27564576}.
CC -!- SIMILARITY: Belongs to the ACDP family. {ECO:0000305}.
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DR EMBL; BX284604; CAM36329.1; -; Genomic_DNA.
DR RefSeq; NP_503052.1; NM_070651.3.
DR AlphaFoldDB; A3QM97; -.
DR SMR; A3QM97; -.
DR STRING; 6239.C52D10.12.1; -.
DR iPTMnet; A3QM97; -.
DR EPD; A3QM97; -.
DR PaxDb; A3QM97; -.
DR PeptideAtlas; A3QM97; -.
DR EnsemblMetazoa; C52D10.12.1; C52D10.12.1; WBGene00016879.
DR EnsemblMetazoa; C52D10.12.2; C52D10.12.2; WBGene00016879.
DR GeneID; 178499; -.
DR KEGG; cel:CELE_C52D10.12; -.
DR UCSC; C52D10.12.1; c. elegans.
DR CTD; 178499; -.
DR WormBase; C52D10.12; CE28434; WBGene00016879; cnnm-1.
DR eggNOG; KOG2118; Eukaryota.
DR GeneTree; ENSGT00940000169533; -.
DR HOGENOM; CLU_011310_1_2_1; -.
DR InParanoid; A3QM97; -.
DR OMA; IYGNHLD; -.
DR OrthoDB; 1446644at2759; -.
DR PhylomeDB; A3QM97; -.
DR PRO; PR:A3QM97; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00016879; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:UniProtKB.
DR GO; GO:0010960; P:magnesium ion homeostasis; IGI:UniProtKB.
DR GO; GO:0015693; P:magnesium ion transport; IGI:UniProtKB.
DR GO; GO:1905941; P:positive regulation of gonad development; IGI:UniProtKB.
DR GO; GO:0040018; P:positive regulation of multicellular organism growth; IGI:UniProtKB.
DR GO; GO:0040026; P:positive regulation of vulval development; IGI:UniProtKB.
DR GO; GO:0032026; P:response to magnesium ion; IGI:UniProtKB.
DR CDD; cd04590; CBS_pair_CorC_HlyC_assoc; 1.
DR Gene3D; 3.10.580.10; -; 1.
DR InterPro; IPR045095; ACDP.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR002550; CNNM.
DR InterPro; IPR044751; Ion_transp-like_CBS.
DR PANTHER; PTHR12064; PTHR12064; 1.
DR Pfam; PF01595; DUF21; 1.
DR SUPFAM; SSF54631; SSF54631; 1.
DR PROSITE; PS51371; CBS; 2.
DR PROSITE; PS51846; CNNM; 1.
PE 2: Evidence at transcript level;
KW CBS domain; Cell membrane; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..811
FT /note="Metal transporter cnnm-1"
FT /evidence="ECO:0000305"
FT /id="PRO_5002658165"
FT TOPO_DOM 25..204
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..259
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..284
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..315
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 337..811
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT DOMAIN 197..376
FT /note="CNNM transmembrane"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01193"
FT DOMAIN 394..456
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 462..530
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT REGION 741..760
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 741..757
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 20
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 49
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 122
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 435
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 453
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 745
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 752
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 811 AA; 89495 MW; F9A49FE3031A8296 CRC64;
MSASCLRLLT LSLFILGQCN VTAAQNGVDD EVTTVTAILD SATTAAADNS TVPTQSASNN
NTSQSSKIPT IFGMRVELPA DDPFGYDKHG VCSVTPEEEF KVVIYGNHLD KIHQIIWTFT
NNCSEPAYVI DALNHFKVHF NHKATFHLTL KLLPEMVHAY KMCVKPKVAP GSPPLGEIYP
LDDISTWLTT ERPPKEYFLP LPLQIACIGF LLCLSALFSG LTLGLMSLTP QELELVIKSG
AIKEQKCAAK ILPVRKKGNL LLCSLLLGNV IVNSAISILM GELTTGIYAL IGSTMGIVIF
GEILPQSICV KKGLEVGAHT ISITQLFIFL TFPIAWPVSK LLDCLLGDEY QAYDRKRLME
LIKMSITDNG QVSNELKIAV GAMEIADKVV KDVMTKIEDV FMLPDTTVLN AKTVMEIVKM
GYTRIPVYQY GDKNNVTDML FVKDLALLDP DDNFTVKTVC GYHKHPVKFV MNDTPLPNLL
EAFKKGEGHL AMVKRLINTD DKHDPSYVLV GVVTLEDIVE EILQAEINDE FDIVSDNVNK
VKIKKEQNRD ATKYFGDHEA PQTMISMQLQ MVALQWLVSN ERGFRQEFLD TNVLERLIRS
SARRVDVSAL MAMGDDAINV PRLAKVYTKD ELSDKYILIL EGRIQVTIGA SGMMFEAGPW
HHFGGEIMAK LVDGAATLGR SMSIVGTSEL SARRPDLMFK PDYSAVVKED CTYLEISVSA
YINAYKASLM QRERPLNDLS DVSHNSSAHN SNLSLVEKPG PITDPSAMLV PENVRKPSVV
SMDSPKILVG LGQHPVAPVA EEEEMALLDQ P
