CNNM2_CAEEL
ID CNNM2_CAEEL Reviewed; 762 AA.
AC Q9GYL2;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Metal transporter cnnm-2 {ECO:0000305};
DE AltName: Full=CNNM family homolog 2 {ECO:0000312|WormBase:R04E5.2};
DE Flags: Precursor;
GN Name=cnnm-2 {ECO:0000312|WormBase:R04E5.2};
GN ORFNames=R04E5.2 {ECO:0000312|WormBase:R04E5.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27564576; DOI=10.1371/journal.pgen.1006276;
RA Ishii T., Funato Y., Hashizume O., Yamazaki D., Hirata Y., Nishiwaki K.,
RA Kono N., Arai H., Miki H.;
RT "Mg2+ extrusion from intestinal epithelia by CNNM proteins is essential for
RT gonadogenesis via AMPK-TORC1 signaling in Caenorhabditis elegans.";
RL PLoS Genet. 12:E1006276-E1006276(2016).
CC -!- FUNCTION: Probable metal transporter. Probably acts redundantly with
CC the other metal transport proteins cnnm-1, cnnm-3, cnnm-4 and cnnm-5 to
CC regulate Mg(2+) homeostasis. {ECO:0000305|PubMed:27564576}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Double knockout with cnnm-3
CC results in 22% sterility. Quintuple knockout with cnnm-1, cnnm-3, cnnm-
CC 4 and cnnm-5 results in a reduced lifespan and 100% sterility.
CC {ECO:0000269|PubMed:27564576}.
CC -!- SIMILARITY: Belongs to the ACDP family. {ECO:0000305}.
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DR EMBL; BX284606; CCD65954.1; -; Genomic_DNA.
DR RefSeq; NP_509493.1; NM_077092.3.
DR AlphaFoldDB; Q9GYL2; -.
DR SMR; Q9GYL2; -.
DR STRING; 6239.R04E5.2; -.
DR EPD; Q9GYL2; -.
DR PaxDb; Q9GYL2; -.
DR PeptideAtlas; Q9GYL2; -.
DR EnsemblMetazoa; R04E5.2.1; R04E5.2.1; WBGene00019869.
DR UCSC; R04E5.2; c. elegans.
DR WormBase; R04E5.2; CE28746; WBGene00019869; cnnm-2.
DR eggNOG; KOG2118; Eukaryota.
DR HOGENOM; CLU_011310_1_2_1; -.
DR InParanoid; Q9GYL2; -.
DR OMA; INLMKMT; -.
DR OrthoDB; 1446644at2759; -.
DR PhylomeDB; Q9GYL2; -.
DR PRO; PR:Q9GYL2; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00019869; Expressed in material anatomical entity and 3 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008340; P:determination of adult lifespan; IGI:UniProtKB.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0010960; P:magnesium ion homeostasis; IEA:InterPro.
DR GO; GO:1905941; P:positive regulation of gonad development; IGI:UniProtKB.
DR Gene3D; 3.10.580.10; -; 1.
DR InterPro; IPR045095; ACDP.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR002550; CNNM.
DR PANTHER; PTHR12064; PTHR12064; 1.
DR Pfam; PF01595; DUF21; 1.
DR SUPFAM; SSF54631; SSF54631; 1.
DR PROSITE; PS51846; CNNM; 1.
PE 3: Inferred from homology;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..762
FT /note="Metal transporter cnnm-2"
FT /evidence="ECO:0000305"
FT /id="PRO_5004327911"
FT TOPO_DOM 22..153
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..208
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 252..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 262..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 283..762
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT DOMAIN 145..323
FT /note="CNNM transmembrane"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01193"
FT DOMAIN 344..408
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 444..514
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT REGION 710..736
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 302
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 405
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 530
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 594
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 669
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 762 AA; 85182 MW; B1A2A93275FB6708 CRC64;
MIIKVFLRLL LLCAHIVCID GKLEIRPVVS GVRIESDVDA SGFLGYGDAG ELLVEANTEV
DLVIFGHGLE NVEMVTFTDS VCVTSEFNVS ESTFYIHKDM KIVFKYAFVA WPQPWRICLK
SECHGLIQID DDRTWIQAVQ STHETFMPVW AQCAILCLLF SISALCSGLT LGLMALTPQE
LSILMKSGSQ REKKHAAAIY PIRCHGNRLL CTVIIMNVIV NTGITLLFDD LAEGLIAFVA
STVGIVVFGE ILPQSICVKY GLAVGANTIF ITKFFMFLLF PITWPLGKIL DKYAGVDIDV
VNRSRMVEML KMNMENDACD IDLSTLKIAI GAMELTKKSV RDVMTDIDDV FMLSEDQVLN
AETMTKISDS GYTRIPVFEG NNRNKVAVKN LLYVSDLALI GKDNNITVKA VARFNKRRLR
IVDESMPLTA LMDEFKLGDY HLAMVAKATE VKKHHHGKFA DGTVDSFILK SMKLVEATMM
PQVENPEDHP VTLVGLITLE DITEELLQAE ITDETDCYVT DDAQKKRRTN TSKKSAAELF
CSEKKSERLS LHMLEMTEKW LLEKTPLFGN MNPKAFENLI QRNIREVLIV PPKNSTSPGT
LNLFEAGVMS KRFLLILEGK ATIRFNEKDL IFECGPWTCF GEAILEKMEM CISDRKEPST
GFFFLPDYNL TVSGPCRFLQ ISTSSLLHSL RITQFVKEIR TPKISITSDD DFGSPTRKAS
ILDSSPNSRK RSSTSVMNSL ALPTARLAAK IASVEELKPL ME
