CNNM4_CAEEL
ID CNNM4_CAEEL Reviewed; 467 AA.
AC Q17586;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 29-OCT-2014, sequence version 3.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Metal transporter cnnm-4 {ECO:0000305};
DE AltName: Full=CNNM family homolog 4 {ECO:0000312|WormBase:C01H6.6};
GN Name=cnnm-4 {ECO:0000312|WormBase:C01H6.6};
GN ORFNames=C01H6.6 {ECO:0000312|WormBase:C01H6.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27564576; DOI=10.1371/journal.pgen.1006276;
RA Ishii T., Funato Y., Hashizume O., Yamazaki D., Hirata Y., Nishiwaki K.,
RA Kono N., Arai H., Miki H.;
RT "Mg2+ extrusion from intestinal epithelia by CNNM proteins is essential for
RT gonadogenesis via AMPK-TORC1 signaling in Caenorhabditis elegans.";
RL PLoS Genet. 12:E1006276-E1006276(2016).
CC -!- FUNCTION: Probable metal transporter. Probably acts redundantly with
CC the other metal transport proteins cnnm-1, cnnm-2, cnnm-3 and cnnm-5 to
CC regulate Mg(2+) homeostasis. {ECO:0000305|PubMed:27564576}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Quintuple knockout with
CC cnnm-1, cnnm-2, cnnm-3 and cnnm-5 results in a reduced lifespan and
CC 100% sterility. {ECO:0000269|PubMed:27564576}.
CC -!- SIMILARITY: Belongs to the ACDP family. {ECO:0000305}.
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DR EMBL; BX284601; CAA95784.3; -; Genomic_DNA.
DR PIR; T18850; T18850.
DR RefSeq; NP_492040.3; NM_059639.3.
DR AlphaFoldDB; Q17586; -.
DR SMR; Q17586; -.
DR STRING; 6239.C01H6.6.2; -.
DR PaxDb; Q17586; -.
DR EnsemblMetazoa; C01H6.6.1; C01H6.6.1; WBGene00007255.
DR GeneID; 182093; -.
DR KEGG; cel:CELE_C01H6.6; -.
DR UCSC; C01H6.6; c. elegans.
DR CTD; 182093; -.
DR WormBase; C01H6.6; CE50057; WBGene00007255; cnnm-4.
DR eggNOG; KOG2118; Eukaryota.
DR HOGENOM; CLU_467891_0_0_1; -.
DR InParanoid; Q17586; -.
DR OMA; MHAINVV; -.
DR OrthoDB; 1446644at2759; -.
DR PRO; PR:Q17586; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00007255; Expressed in adult organism and 2 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008340; P:determination of adult lifespan; IGI:UniProtKB.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0010960; P:magnesium ion homeostasis; IEA:InterPro.
DR GO; GO:1905941; P:positive regulation of gonad development; IGI:UniProtKB.
DR CDD; cd04590; CBS_pair_CorC_HlyC_assoc; 1.
DR Gene3D; 3.10.580.10; -; 1.
DR InterPro; IPR045095; ACDP.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR002550; CNNM.
DR InterPro; IPR044751; Ion_transp-like_CBS.
DR PANTHER; PTHR12064; PTHR12064; 2.
DR Pfam; PF01595; DUF21; 1.
DR SUPFAM; SSF54631; SSF54631; 1.
DR PROSITE; PS51371; CBS; 2.
DR PROSITE; PS51846; CNNM; 1.
PE 3: Inferred from homology;
KW CBS domain; Cell membrane; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..467
FT /note="Metal transporter cnnm-4"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438476"
FT TOPO_DOM 1..110
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 132..170
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..196
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 218..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..467
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT DOMAIN 107..293
FT /note="CNNM transmembrane"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01193"
FT DOMAIN 317..381
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 394..461
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 364
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 467 AA; 53072 MW; E5A13BDF16676A59 CRC64;
MELYAAGRYD SEPFKMNEHD IIQLYGEDVQ YVKNAFLSSD STCNNNVYSL EEHELDDDKK
NLSGVLVEYK IKISLLQSSD GVKHLFFCTN PKSVDIARML EIPEGKDKTR VYFMMPLLVL
CLGLSATFSG LNLAIMSFSI NDLKLIQESD SDKLMKQRAM DVMRLRRNSN FVLVTIIFGN
CFCNISITLL MNYFAEFYGF GGFIFVELIS TALLLIFTEI LPSLIFTKNA LAIASRLQYF
VIFTMCITSP ISYPLAMLLN IILGKENADD SAPLDLDALQ IDELEDEEAA DGNNFHEMMS
VVKKTIKLRE KLASDVMTEI DKVGMYSEHQ QVTHSFLLDA YEQGHSRLPV YEGETRNKIR
GVLNITDMML LMDDEGRGSD TDLTLGTMLS VLEKRRKHCF VLDTMPVEHF MSELQQGCPM
AIVVRYKEVD SEEDGTEIYE VCGIVTLEDC IEEILGEIFD EKDARQE
