CNO10_DROME
ID CNO10_DROME Reviewed; 635 AA.
AC Q9V3G6; Q8SZP3;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=CCR4-NOT transcription complex subunit 10;
DE Short=CNOT10;
GN Name=Not10; ORFNames=CG18616;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-45, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [5]
RP IDENTIFICATION IN THE CCR4-NOT COMPLEX.
RX PubMed=20504953; DOI=10.1261/rna.2145110;
RA Temme C., Zhang L., Kremmer E., Ihling C., Chartier A., Sinz A.,
RA Simonelig M., Wahle E.;
RT "Subunits of the Drosophila CCR4-NOT complex and their roles in mRNA
RT deadenylation.";
RL RNA 16:1356-1370(2010).
RN [6]
RP IDENTIFICATION IN THE CCR4-NOT COMPLEX.
RX PubMed=23303381; DOI=10.4161/rna.23018;
RA Bawankar P., Loh B., Wohlbold L., Schmidt S., Izaurralde E.;
RT "NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT complex
RT that docks onto the NOT1 N-terminal domain.";
RL RNA Biol. 10:228-244(2013).
CC -!- FUNCTION: Component of the CCR4-NOT complex which is one of the major
CC cellular mRNA deadenylases and is linked to various cellular processes
CC including bulk mRNA degradation, miRNA-mediated repression,
CC translational repression during translational initiation and general
CC transcription regulation. Additional complex functions may be a
CC consequence of its influence on mRNA expression. Is not required for
CC association of CNOT7 to the CCR4-NOT complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the CCR4-NOT complex. CNOT10 and CNOT11 form a
CC subcomplex docked to the CNOT1 scaffold. {ECO:0000269|PubMed:20504953,
CC ECO:0000269|PubMed:23303381}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CNOT10 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL48085.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF54839.1; -; Genomic_DNA.
DR EMBL; AY070614; AAL48085.1; ALT_FRAME; mRNA.
DR RefSeq; NP_001303491.1; NM_001316562.1.
DR RefSeq; NP_650215.1; NM_141958.4.
DR AlphaFoldDB; Q9V3G6; -.
DR BioGRID; 66647; 19.
DR IntAct; Q9V3G6; 1.
DR STRING; 7227.FBpp0082112; -.
DR iPTMnet; Q9V3G6; -.
DR PaxDb; Q9V3G6; -.
DR EnsemblMetazoa; FBtr0082643; FBpp0082112; FBgn0260444.
DR EnsemblMetazoa; FBtr0445304; FBpp0401462; FBgn0260444.
DR GeneID; 41552; -.
DR KEGG; dme:Dmel_CG18616; -.
DR UCSC; CG18616-RA; d. melanogaster.
DR CTD; 41552; -.
DR FlyBase; FBgn0260444; Not10.
DR VEuPathDB; VectorBase:FBgn0260444; -.
DR eggNOG; KOG2471; Eukaryota.
DR GeneTree; ENSGT00390000001827; -.
DR HOGENOM; CLU_013100_2_0_1; -.
DR InParanoid; Q9V3G6; -.
DR OMA; AHSCEIL; -.
DR OrthoDB; 249353at2759; -.
DR PhylomeDB; Q9V3G6; -.
DR SignaLink; Q9V3G6; -.
DR BioGRID-ORCS; 41552; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41552; -.
DR PRO; PR:Q9V3G6; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0260444; Expressed in embryonic/larval hemocyte (Drosophila) and 23 other tissues.
DR ExpressionAtlas; Q9V3G6; baseline and differential.
DR Genevisible; Q9V3G6; DM.
DR GO; GO:0030014; C:CCR4-NOT complex; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR GO; GO:0006402; P:mRNA catabolic process; IDA:FlyBase.
DR GO; GO:0017148; P:negative regulation of translation; IDA:FlyBase.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR039740; CNOT10.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR12979; PTHR12979; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome;
KW RNA-mediated gene silencing; Transcription; Transcription regulation;
KW Translation regulation.
FT CHAIN 1..635
FT /note="CCR4-NOT transcription complex subunit 10"
FT /id="PRO_0000372844"
FT REGION 396..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 450..474
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 131..165
FT /evidence="ECO:0000255"
FT MOD_RES 45
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT CONFLICT 347
FT /note="L -> F (in Ref. 3; AAL48085)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 635 AA; 69693 MW; CCE078AC0D8E7554 CRC64;
MDSAESPTKT QAGEDENYSL LCQAHEQFNN SEFDRCLELL QELETRGESS GPVLRHNRAV
VSYYKTGCTQ HSVLLKELEA LTADADAPGD VSSGLSLKQG AAAATVARYN RAVIYYHRHM
FGTALEKLAP LVARLEALEK AMAALVATLQ LQLLLATNQL NRAEAFLDYL QYKLNLVATA
PSSNAAEEAA VVGTAPPSAA TNSSVVASPG GAAVEGAVPG LGGSLQLLQL ITLVLNRKPV
VIQEDGTPEY AALKAQQYYI MKDFQMAAKQ LMRINNECTQ AGTITPQLST CIANNMGVIH
LRVRHYAIAA KFFQNALNFD QQLARNLRQS TLQTMSSARS CEILYNLGVA MLHLRRPKEA
FQCFLVPVKQ FHSNPRLWLR MAEACIMEHE AKLVEEERQS QSETPSTKPY APQSAGVPEP
TLEFAVLCLR SALTLTQHYK TRFHMAAVSS EDVEAPEPKD PTQESWRHPQ DNNFCNPSKP
VSLESLENMM AAIYAAHSFV SLRLGDHVTA LEMSEKLLAC ERLSDAHKLL GHMYAGEALM
LMDKASEARD HLDPTFVGTL NAFDFETRDW QLKSVDAAQN VVRYNLAVAM ALQNDLPQAK
ALLANLTHSL VANKALALRR FMDLKMGPVP GGTPS
