CNOT1_DANRE
ID CNOT1_DANRE Reviewed; 2374 AA.
AC A1A5H6; Q6DRB0;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=CCR4-NOT transcription complex subunit 1;
DE AltName: Full=CCR4-associated factor 1;
GN Name=cnot1; ORFNames=zgc:152902;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1595.
RX PubMed=15256591; DOI=10.1073/pnas.0403929101;
RA Amsterdam A., Nissen R.M., Sun Z., Swindell E.C., Farrington S.,
RA Hopkins N.;
RT "Identification of 315 genes essential for early zebrafish development.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:12792-12797(2004).
CC -!- FUNCTION: Scaffolding component of the CCR4-NOT complex which is one of
CC the major cellular mRNA deadenylases and is linked to various cellular
CC processes including bulk mRNA degradation, miRNA-mediated repression,
CC translational repression during translational initiation and general
CC transcription regulation. Additional complex functions may be a
CC consequence of its influence on mRNA expression. Its scaffolding
CC function implies its interaction with the catalytic complex module and
CC diverse RNA-binding proteins mediating the complex recruitment to
CC selected mRNA 3'UTRs. Acts as a transcriptional repressor. Represses
CC the ligand-dependent transcriptional activation by nuclear receptors
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the CCR4-NOT complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- DOMAIN: Contains Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, a motif known to
CC be important for the association with nuclear receptors. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CNOT1 family. {ECO:0000305}.
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DR EMBL; BC128655; AAI28656.1; -; mRNA.
DR EMBL; AY648849; AAT68167.1; -; mRNA.
DR RefSeq; NP_001073420.1; NM_001079951.2.
DR AlphaFoldDB; A1A5H6; -.
DR SMR; A1A5H6; -.
DR STRING; 7955.ENSDARP00000004298; -.
DR PaxDb; A1A5H6; -.
DR PeptideAtlas; A1A5H6; -.
DR PRIDE; A1A5H6; -.
DR GeneID; 448949; -.
DR KEGG; dre:448949; -.
DR CTD; 23019; -.
DR ZFIN; ZDB-GENE-040915-1; cnot1.
DR eggNOG; KOG1831; Eukaryota.
DR InParanoid; A1A5H6; -.
DR OrthoDB; 42530at2759; -.
DR PhylomeDB; A1A5H6; -.
DR Reactome; R-DRE-429947; Deadenylation of mRNA.
DR Reactome; R-DRE-6804115; TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.
DR PRO; PR:A1A5H6; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0030014; C:CCR4-NOT complex; ISS:UniProtKB.
DR GO; GO:0030015; C:CCR4-NOT core complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000932; C:P-body; ISS:UniProtKB.
DR GO; GO:0060090; F:molecular adaptor activity; IBA:GO_Central.
DR GO; GO:0030331; F:nuclear estrogen receptor binding; ISS:UniProtKB.
DR GO; GO:0042974; F:nuclear retinoic acid receptor binding; ISS:UniProtKB.
DR GO; GO:0048589; P:developmental growth; IMP:ZFIN.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR GO; GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0048387; P:negative regulation of retinoic acid receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0000288; P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IBA:GO_Central.
DR GO; GO:0010606; P:positive regulation of cytoplasmic mRNA processing body assembly; ISS:UniProtKB.
DR GO; GO:1900153; P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; ISS:UniProtKB.
DR GO; GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; ISS:UniProtKB.
DR Gene3D; 1.25.40.840; -; 1.
DR InterPro; IPR007196; CCR4-Not_Not1_C.
DR InterPro; IPR032191; CNOT1_CAF1_bind.
DR InterPro; IPR024557; CNOT1_dom_4.
DR InterPro; IPR032194; CNOT1_HEAT.
DR InterPro; IPR032193; CNOT1_TTP_bind.
DR InterPro; IPR038535; CNOT1_TTP_bind_sf.
DR InterPro; IPR040398; Not1.
DR PANTHER; PTHR13162; PTHR13162; 1.
DR Pfam; PF16415; CNOT1_CAF1_bind; 1.
DR Pfam; PF16418; CNOT1_HEAT; 1.
DR Pfam; PF16417; CNOT1_TTP_bind; 1.
DR Pfam; PF12842; DUF3819; 1.
DR Pfam; PF04054; Not1; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome; Repressor;
KW RNA-mediated gene silencing; Transcription; Transcription regulation;
KW Translation regulation.
FT CHAIN 1..2374
FT /note="CCR4-NOT transcription complex subunit 1"
FT /id="PRO_0000315543"
FT REGION 1009..1060
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1082..1604
FT /note="Interaction with CCR4-NOT complex catalytic
FT subunits"
FT /evidence="ECO:0000250"
FT MOTIF 153..157
FT /note="LXXLL"
FT MOTIF 181..185
FT /note="LXXLL"
FT MOTIF 223..227
FT /note="LXXLL"
FT MOTIF 570..574
FT /note="LXXLL"
FT MOTIF 1638..1642
FT /note="LXXLL"
FT MOTIF 1940..1944
FT /note="LXXLL"
FT MOTIF 2094..2098
FT /note="LXXLL"
FT COMPBIAS 1009..1050
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 424
FT /note="N -> Y (in Ref. 2; AAT68167)"
FT /evidence="ECO:0000305"
FT CONFLICT 433
FT /note="T -> I (in Ref. 2; AAT68167)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2374 AA; 266604 MW; CAE9127310F44516 CRC64;
MNLDSLSLAL SQISYLVDNL TKKNYRASQQ EIQHIVNRHG PEADRHLLRC LFSHVDFSGD
GKSSGKDFHQ TQFLIQECVS LITKPNFIST LCYAIDNPLH YQKSLKPSPH LFTQLSKVLK
LSKVQEVILG LALSNSSNAD LRGFAAQFVK QKLPDLLRSY VDADLGGNQE GGFQDIAIEV
LHLLLSHLLF GQKGSSGVGQ EQIDAFLKTL CRDFPQERCP VVLAPLLYPD KRDILMDRIL
PDSGDLNKTM MESSLADFMQ EVGYGFCASL EECRNIILQY GVREVTASQV ARVLGMMART
HSGLSDGISL QTITNPVGGG GIWSDGKDKS DSSQAWNVEV LIDVVKEVNP NLNFKEVTYE
LDHPGFLIRD SKGLQIVVYG IQRGLGMEVF PVDLIYRPWK HAEGQLSFIQ HSLLSPEVFC
FADNPCHTVA IDTLKAPPED DNREIATWKS LDLVESLLRL SEVGHYEQVK QLFSFPIKHC
PDMLVLALLQ ISTSWHTLRH ELISTLMPIF LGNHPNSAII LHYAWHGQGQ SPSIRQLIMH
SMAEWYMRGE QYDQAKLSRI LDVAQDLKSL SMLLNGTPFA FVIDLAALAS RREYLKLDKW
LTDKIREHGE PFIQACVTFL KRRCPSIMGG LAPEKDQPKS AQLPPETLAT MLACLQSCAG
SVSQELSETI LTMVANCSNV MNKARQPPPG VLPKGRAPST SSLDAISPVQ MDPLSAMGSL
SLGVSSTSHT PSMQGFPSLQ GSAFSNPQSP AKAFSNLPNP NPSTAFPGIN PLSSQLQGPL
STSLSGIGSG LGMPTVSSDV FSARKMSTPG LNPPTFQQTD LSQVWPEANQ HFSKEIDDEA
NSYFQRIYNH PPHPTMSVDE VLEMLQRFKD SNIKREREVF NCMLRNLFEE YRFFPQYPDK
ELHITACLFG GIIEKGLVTY MALGLALRYV LEALRKPFGS KMYYFGIAAL DRFKNRLKDY
PQYCQHLASI AHFLQFPHHL QEYIEYGQQS RDPPVKMQGS ITTPGSLALA QAQAQSQPPK
APQPGQASTL VTTATTTTTA AKTTTITRPT AVGPKKDVPP SINTTNIDTL LVATDQTERI
VEPPENVQEK IAFIFNNLSQ SNMSQKVEEL KETVKEEFMP WVSQYLVMKR VSIEPNFHSL
YSNFLDTLKN PEFVKMVLNE TYRNIKVLLT SDKAAANFSD RSLLKNLGHW LGMITLAKNK
PILYTDLELK SLLLEAYVKG QQELLYVVPF VAKVLESSLR SVIFRPQNPW TMGIMNVLAE
LHQEHDLKLN LKFEIEVLCK NLSMDITDLK PGNLLRDKDK LKTLEEQLSA PKKETKPPEE
LLPIVTTDSV PFTAAPSTPA TTTACTATGP PTPQFSYHDI NVYALAGLAP HININVNIPL
LQAHPQLKQC VRPAIERAVQ ELVHPVVDRS IKIAMTTCEQ IVRKDFALDS EESHMRVAAH
HMMRNLTAGM AMITCREPLL MSIATNLKNS FAAALRAPTP QQREMMEEAA ARIAQDNCEL
ACCFIQKTAV EKAGPEMDKR LATEFELRKH ARQEGRRYCD PMVLTYQAER MPEQIRLKVG
GVDPKQLAVY EEFARNVPGF LPSNDLSQPT GFLAQPMKQQ AWPTDDVAHI YEKCISDLEQ
HLHAIPPALA MNPQTQAIRS LLEAVVMARN SRDGIAALGL LQKAVEGLLD ATSGADPELL
LSYRECHLLV LKALQDGRAY GPQWCNKQIT RCLIECRDEY KYNVEAVELL IRNHLVNMQQ
YDLHLAQSME NGLNYMAVAF AMQLVKLLLV DERSVSHITE ADLFHTIETL MRTSAHSRAN
APEGLPQLMD VVRSNYEAMI DRHHGGPNFM MHSGISQASE YDDPPGLREK AEYLLREWVN
LYHSAAAGRD STKAFSAFVG QMHQQGILKT DDLITRFFRL CTEMCVEISY RAQAEQQHPT
TSPAIIRAKC YHNLDAFVRL IALLVKHSGE ATNTVTKINL LNKVLGIVVG VLIQDHDVRQ
TEFQQLPYHR IFIMLLLELN APEHVLETIN FQTLTAFCNT FHILRPTKAP GFVYAWLELI
SHRIFIARML AHTPQQKGWP MYAQLLIDLF KYLAPFLRNV ELNKPMQILY KGTLRVLLVL
LHDFPEFLCD YHYGFCDVIP PNCIQLRNLI LSAFPRNMRL PDPFTPNLKV DMLSEINIAP
RILTNFTGVM PSQFKKDLDS YLKTRSPVTF LSELRSNLQV SNEPGNRYNI QLINALVPYV
GTQAIAHIHN KGSTPSMSTI THSAHMDIFQ NLAVDLDTEG RYLFLNAIAN QLRYPNSHTH
YFSCTMLYLF AEANAEAIQE QITRVLLERL IVNRPHPWGL LITFIELIKN PAFKFWSHDF
VHCAPEIEKL FQSVAQCCMG QKQAQQVMEG TGAS
