CNOT3_MOUSE
ID CNOT3_MOUSE Reviewed; 751 AA.
AC Q8K0V4; B2RUA7;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=CCR4-NOT transcription complex subunit 3;
DE AltName: Full=CCR4-associated factor 3;
GN Name=Cnot3; Synonyms=Not3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, Embryo, and Retina;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [3]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH NANOS2.
RX PubMed=20133598; DOI=10.1073/pnas.0908664107;
RA Suzuki A., Igarashi K., Aisaki K., Kanno J., Saga Y.;
RT "NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to
RT suppression of specific RNAs.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:3594-3599(2010).
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=21897366; DOI=10.1038/emboj.2011.320;
RA Morita M., Oike Y., Nagashima T., Kadomatsu T., Tabata M., Suzuki T.,
RA Nakamura T., Yoshida N., Okada M., Yamamoto T.;
RT "Obesity resistance and increased hepatic expression of catabolism-related
RT mRNAs in Cnot3+/- mice.";
RL EMBO J. 30:4678-4691(2011).
RN [5]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=22367759; DOI=10.1002/stem.1070;
RA Zheng X., Dumitru R., Lackford B.L., Freudenberg J.M., Singh A.P.,
RA Archer T.K., Jothi R., Hu G.;
RT "Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit
RT extraembryonic differentiation.";
RL Stem Cells 30:910-922(2012).
CC -!- FUNCTION: Component of the CCR4-NOT complex which is one of the major
CC cellular mRNA deadenylases and is linked to various cellular processes
CC including bulk mRNA degradation, miRNA-mediated repression,
CC translational repression during translational initiation and general
CC transcription regulation. Additional complex functions may be a
CC consequence of its influence on mRNA expression. May be involved in
CC metabolic regulation; may be involved in recruitment of the CCR4-NOT
CC complex to deadenylation target mRNAs involved in energy metabolism.
CC Involved in mitotic progression and regulation of the spindle assembly
CC checkpoint by regulating the stability of MAD1L1 mRNA. Can repress
CC transcription and may link the CCR4-NOT complex to transcriptional
CC regulation; the repressive function may involve histone deacetylases.
CC Involved in the maintenance of embryonic stem (ES) cell identity;
CC prevents their differentiation towards extraembryonic trophectoderm
CC lineages. {ECO:0000269|PubMed:21897366, ECO:0000269|PubMed:22367759}.
CC -!- SUBUNIT: Component of the CCR4-NOT complex; distinct complexes seem to
CC exist that differ in the participation of probably mutually exclusive
CC catalytic subunits. In the complex interacts directly with CNOT2.
CC Interacts with TIP120B and NANOS2 (PubMed:20133598). Interacts with
CC EBF1 (By similarity). {ECO:0000250|UniProtKB:O75175,
CC ECO:0000269|PubMed:20133598}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:20133598}. Cytoplasm,
CC P-body {ECO:0000269|PubMed:20133598}. Note=NANOS2 promotes its
CC localization to P-body.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryonic stem (ES) cells and in
CC inner cell mass (ICM) of the blastocyst. {ECO:0000269|PubMed:22367759}.
CC -!- INDUCTION: Decreased levels in liver and white adipose tissues upon
CC fasting,. {ECO:0000269|PubMed:21897366}.
CC -!- SIMILARITY: Belongs to the CNOT2/3/5 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC030332; AAH30332.1; -; mRNA.
DR EMBL; BC053437; AAH53437.1; -; mRNA.
DR EMBL; BC141041; AAI41042.1; -; mRNA.
DR CCDS; CCDS20721.1; -.
DR RefSeq; NP_666288.1; NM_146176.3.
DR RefSeq; XP_011248803.1; XM_011250501.2.
DR RefSeq; XP_017177635.1; XM_017322146.1.
DR AlphaFoldDB; Q8K0V4; -.
DR SMR; Q8K0V4; -.
DR BioGRID; 231295; 19.
DR DIP; DIP-46844N; -.
DR IntAct; Q8K0V4; 21.
DR MINT; Q8K0V4; -.
DR STRING; 10090.ENSMUSP00000039098; -.
DR iPTMnet; Q8K0V4; -.
DR PhosphoSitePlus; Q8K0V4; -.
DR EPD; Q8K0V4; -.
DR MaxQB; Q8K0V4; -.
DR PaxDb; Q8K0V4; -.
DR PeptideAtlas; Q8K0V4; -.
DR PRIDE; Q8K0V4; -.
DR ProteomicsDB; 283407; -.
DR Antibodypedia; 1756; 260 antibodies from 29 providers.
DR DNASU; 232791; -.
DR Ensembl; ENSMUST00000038913; ENSMUSP00000039098; ENSMUSG00000035632.
DR GeneID; 232791; -.
DR KEGG; mmu:232791; -.
DR UCSC; uc009evl.2; mouse.
DR CTD; 4849; -.
DR MGI; MGI:2385261; Cnot3.
DR VEuPathDB; HostDB:ENSMUSG00000035632; -.
DR eggNOG; KOG2150; Eukaryota.
DR GeneTree; ENSGT00390000014743; -.
DR HOGENOM; CLU_013819_1_1_1; -.
DR InParanoid; Q8K0V4; -.
DR OMA; FEYESWN; -.
DR OrthoDB; 1109812at2759; -.
DR PhylomeDB; Q8K0V4; -.
DR TreeFam; TF321963; -.
DR Reactome; R-MMU-429947; Deadenylation of mRNA.
DR Reactome; R-MMU-6804115; TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.
DR BioGRID-ORCS; 232791; 28 hits in 77 CRISPR screens.
DR ChiTaRS; Cnot3; mouse.
DR PRO; PR:Q8K0V4; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8K0V4; protein.
DR Bgee; ENSMUSG00000035632; Expressed in animal zygote and 196 other tissues.
DR ExpressionAtlas; Q8K0V4; baseline and differential.
DR Genevisible; Q8K0V4; MM.
DR GO; GO:0030014; C:CCR4-NOT complex; ISS:UniProtKB.
DR GO; GO:0030015; C:CCR4-NOT core complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000932; C:P-body; IDA:UniProtKB.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR GO; GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IBA:GO_Central.
DR GO; GO:0120162; P:positive regulation of cold-induced thermogenesis; IMP:YuBioLab.
DR GO; GO:2000036; P:regulation of stem cell population maintenance; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0001829; P:trophectodermal cell differentiation; IMP:UniProtKB.
DR Gene3D; 2.30.30.1020; -; 1.
DR InterPro; IPR038635; CCR4-NOT_su2/3/5_N_sf.
DR InterPro; IPR012270; CCR4-NOT_su3/5.
DR InterPro; IPR040168; Not2/3/5.
DR InterPro; IPR007282; NOT2/3/5_C.
DR InterPro; IPR007207; Not_N.
DR PANTHER; PTHR23326; PTHR23326; 2.
DR Pfam; PF04153; NOT2_3_5; 1.
DR Pfam; PF04065; Not3; 1.
DR PIRSF; PIRSF005290; NOT_su_3_5; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Nucleus; Phosphoprotein;
KW Reference proteome; Repressor; RNA-mediated gene silencing; Transcription;
KW Transcription regulation; Translation regulation.
FT CHAIN 1..751
FT /note="CCR4-NOT transcription complex subunit 3"
FT /id="PRO_0000198334"
FT REGION 240..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 659..751
FT /note="Repressor domain"
FT /evidence="ECO:0000250"
FT COMPBIAS 240..279
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..316
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..348
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 391..494
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 292
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O75175"
FT MOD_RES 299
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 540
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75175"
SQ SEQUENCE 751 AA; 81946 MW; 47C2A7321EDAB13A CRC64;
MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK EIKKLQRLRD
QIKTWVASNE IKDKRQLIEN RKLIETQMER FKVVERETKT KAYSKEGLGL AQKVDPAQKE
KEEVGQWLTN TIDTLNMQVD QFESEVESLS VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV
RMLETILRML DNDSILVDAI RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA
TSPPSHSHME DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP
AKNGSKPVHS NQHPQSPAVP PTYPSGPPPT TSALSSTPGN NGASTPAAPT SALGPKASPA
PSHNSGTPAP YAQAVAPPNA SGPSNAQPRP PSAQPSGGSG GGSGGSSSNS NSGTGGGAGK
QNGATSYSSV VADSPAEVTL SSSGGSSASS QALGPTSGPH NPAPSTSKES STAAPSGAGN
VASGSGNNSG GPSLLVPLPV NPPSSPTPSF SEAKAAGTLL NGPPQFSTTP EIKAPEPLSS
LKSMAERAAI SSGIEDPVPT LHLTDRDIIL SSTSAPPTSS QPPLQLSEVN IPLSLGVCPL
GPVSLTKEQL YQQAMEEAAW HHMPHPSDSE RIRQYLPRNP CPTPPYHHQM PPPHSDTVEF
YQRLSTETLF FIFYYLEGTK AQYLAAKALK KQSWRFHTKY MMWFQRHEEP KTITDEFEQG
TYIYFDYEKW GQRKKEGFTF EYRYLEDRDL Q
