ID   CNPDE_HELPY             Reviewed;         348 AA.
AC   O25683;
DT   11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=3',5'-cyclic-nucleotide phosphodiesterase;
DE            EC=3.1.4.17;
DE   AltName: Full=cAMP/cGMP phosphodiesterase;
GN   OrderedLocusNames=HP_1042;
OS   Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA   Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA   Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA   McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA   Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA   Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA   Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY,
RP   COFACTOR, SUBSTRATE SPECIFICITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=23825549; DOI=10.1371/journal.pone.0066605;
RA   Choi H.P., Juarez S., Ciordia S., Fernandez M., Bargiela R., Albar J.P.,
RA   Mazumdar V., Anton B.P., Kasif S., Ferrer M., Steffen M.;
RT   "Biochemical characterization of hypothetical proteins from Helicobacter
RT   pylori.";
RL   PLoS ONE 8:E66605-E66605(2013).
CC   -!- FUNCTION: Hydrolyzes cAMP to 5'-AMP and cGMP to 5'-GMP. Does not show
CC       phosphohydrolase activity toward various phosphatidylcholine and
CC       phosphorylated sugars. {ECO:0000269|PubMed:23825549}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-
CC         phosphate + H(+); Xref=Rhea:RHEA:14653, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57867, ChEBI:CHEBI:58464; EC=3.1.4.17;
CC         Evidence={ECO:0000269|PubMed:23825549};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000269|PubMed:23825549};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.88 mM for cAMP {ECO:0000269|PubMed:23825549};
CC         KM=0.82 mM for cGMP {ECO:0000269|PubMed:23825549};
CC         KM=0.53 mM for bis-p-nitrophenylphosphate
CC         {ECO:0000269|PubMed:23825549};
CC         Note=kcat is 2008 min(-1) and 957 min(-1) with cAMP and cGMP as
CC         substrate, respectively.;
CC       pH dependence:
CC         Optimum pH is 4.0-4.5. {ECO:0000269|PubMed:23825549};
CC       Temperature dependence:
CC         Optimum temperature is 30-40 degrees Celsius.
CC         {ECO:0000269|PubMed:23825549};
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DR   EMBL; AE000511; AAD08096.1; -; Genomic_DNA.
DR   PIR; B64650; B64650.
DR   RefSeq; NP_207832.1; NC_000915.1.
DR   RefSeq; WP_000984056.1; NC_018939.1.
DR   AlphaFoldDB; O25683; -.
DR   SMR; O25683; -.
DR   STRING; 85962.C694_05390; -.
DR   PaxDb; O25683; -.
DR   EnsemblBacteria; AAD08096; AAD08096; HP_1042.
DR   KEGG; hpy:HP_1042; -.
DR   PATRIC; fig|85962.8.peg.1091; -.
DR   eggNOG; COG2404; Bacteria.
DR   OMA; NANYGRE; -.
DR   SABIO-RK; O25683; -.
DR   Proteomes; UP000000429; Chromosome.
DR   GO; GO:0004114; F:3',5'-cyclic-nucleotide phosphodiesterase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   InterPro; IPR038763; DHH_sf.
DR   SUPFAM; SSF64182; SSF64182; 1.
PE   1: Evidence at protein level;
KW   cAMP; cGMP; Hydrolase; Manganese; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..348
FT                   /note="3',5'-cyclic-nucleotide phosphodiesterase"
FT                   /id="PRO_0000424564"
SQ   SEQUENCE   348 AA;  40223 MW;  BE5DD03EA971AEAB CRC64;
     MMQVYHLSHI DLDGYACQLV SKQFFKNIQC YNANYGREVS ARIYEILNAI AQSKESEFLI
     LVSDLNLNLN EAEYLQDKIQ EHRLQNKNIQ IQLLDHHISG KEVAESFHWY FLDINRCATK
     IVYEFLKKHY AILEPKNTTW LEPLVEMVNS VDIWDTQGYG FELGKVCMRM INQSSELNRF
     MFDDENRNYK LKLLEEVKNY LFLENAPVAY DNDLFKLKKI ALGGDPDAET MDNISSNAQT
     HLLSLKKHDC SVYYQDKKGF LSYSMGGISV LANLFLTQNP DFDFYMDVNA KGNVSLRANG
     NCDVCELSQM CFNGGGHRNA SGGKIDGFRE SFNYRDIKEQ IEEIFNNA