CNPY1_DANRE
ID CNPY1_DANRE Reviewed; 187 AA.
AC Q2L6L1; Q90ZG9;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Protein canopy-1;
DE AltName: Full=Protein D121;
DE Flags: Precursor;
GN Name=cnpy1; Synonyms=d121; ORFNames=si:dkey-265a7.3;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, INTERACTION WITH FGFR1,
RP INDUCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 184-HIS--LEU-187, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=16488878; DOI=10.1016/j.cub.2006.01.055;
RA Hirate Y., Okamoto H.;
RT "Canopy1, a novel regulator of FGF signaling around the midbrain-hindbrain
RT boundary in zebrafish.";
RL Curr. Biol. 16:421-427(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 101-187.
RA Hirate Y., Mieda M., Harada T., Yamasu K., Okamoto H.;
RT "A systematic search for the downstream target genes of the midbrain-MHB
RT reciprocal inductive signaling by ordered differential display revealed the
RT expression of ephrin-A3 in the posterior tectum of zebrafish embryos.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the maintenance of the midbrain-hindbrain
CC boundary (MHB) organizer. Contributes to a positive-feedback loop of
CC FGF signaling in the MHB, enabling the MHB to exert its role as an
CC organizer for the tectal and cerebellar development.
CC {ECO:0000269|PubMed:16488878}.
CC -!- SUBUNIT: Homodimer. Interacts with fgfr1.
CC {ECO:0000269|PubMed:16488878}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000269|PubMed:16488878}.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally from early cleavage stages
CC until the shield stage. Expressed zygotically in the polster at the bud
CC stage. Expressed in the midbrain-hindbrain boundary (MHB) at 12 hours
CC post-fertilization (hpf). Expressed in the tail bud and the mesenchymal
CC cells of the caudal fin primordia at 26 hpf.
CC {ECO:0000269|PubMed:16488878}.
CC -!- INDUCTION: By the fibroblast growth factor fgf8 in the MHB.
CC {ECO:0000269|PubMed:16488878}.
CC -!- SIMILARITY: Belongs to the canopy family. {ECO:0000305}.
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DR EMBL; AB201383; BAE78824.1; -; mRNA.
DR EMBL; BX510360; CAM46989.1; -; Genomic_DNA.
DR EMBL; BC122425; AAI22426.1; -; mRNA.
DR EMBL; AB055671; BAB62803.1; -; mRNA.
DR RefSeq; NP_001034586.1; NM_001039497.2.
DR AlphaFoldDB; Q2L6L1; -.
DR STRING; 7955.ENSDARP00000009432; -.
DR PaxDb; Q2L6L1; -.
DR PRIDE; Q2L6L1; -.
DR Ensembl; ENSDART00000020792; ENSDARP00000009432; ENSDARG00000003757.
DR Ensembl; ENSDART00000163350; ENSDARP00000133952; ENSDARG00000003757.
DR GeneID; 402819; -.
DR KEGG; dre:402819; -.
DR CTD; 285888; -.
DR ZFIN; ZDB-GENE-060315-3; cnpy1.
DR eggNOG; KOG3782; Eukaryota.
DR GeneTree; ENSGT00940000161119; -.
DR HOGENOM; CLU_095726_2_1_1; -.
DR InParanoid; Q2L6L1; -.
DR OMA; TVCERMN; -.
DR OrthoDB; 1417418at2759; -.
DR PhylomeDB; Q2L6L1; -.
DR TreeFam; TF318578; -.
DR PRO; PR:Q2L6L1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 2.
DR Bgee; ENSDARG00000003757; Expressed in testis and 30 other tissues.
DR ExpressionAtlas; Q2L6L1; baseline and differential.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:ZFIN.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:ZFIN.
DR GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IGI:ZFIN.
DR GO; GO:0070121; P:Kupffer's vesicle development; IMP:ZFIN.
DR GO; GO:0030917; P:midbrain-hindbrain boundary development; IMP:ZFIN.
DR GO; GO:0040036; P:regulation of fibroblast growth factor receptor signaling pathway; IMP:ZFIN.
DR InterPro; IPR042415; CNPY.
DR InterPro; IPR021852; DUF3456.
DR PANTHER; PTHR13341; PTHR13341; 1.
DR Pfam; PF11938; DUF3456; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Disulfide bond; Endoplasmic reticulum;
KW Reference proteome; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..187
FT /note="Protein canopy-1"
FT /id="PRO_0000314013"
FT DOMAIN 28..181
FT /note="Saposin B-type"
FT MOTIF 184..187
FT /note="Prevents secretion from ER"
FT /evidence="ECO:0000255"
FT DISULFID 32..177
FT /evidence="ECO:0000250"
FT DISULFID 35..170
FT /evidence="ECO:0000250"
FT DISULFID 90..143
FT /evidence="ECO:0000250"
FT MUTAGEN 184..187
FT /note="HSEL->KDEL: Does not abolish endoplasmic reticulum
FT localization."
FT /evidence="ECO:0000269|PubMed:16488878"
FT MUTAGEN 184..187
FT /note="Missing: Abolishes endoplasmic reticulum
FT localization."
FT /evidence="ECO:0000269|PubMed:16488878"
SQ SEQUENCE 187 AA; 20973 MW; BA9147D3A4BB47E0 CRC64;
MSPWIKHICL VLVAAFMLVK TTESKKDEAL YCSACMAIAD EINYSISQTD PKKMIHVGGF
RLKPDGSLTD KKVPLARSET YLTELLEEVC KSMSDYALYE NPDTKEKSYK RFAPRDNDGG
NFPDFKNFKF DGPESSSALK FACESIVEEL EDDIISLFAS DSDHVAKTLC SEVSDHCKSS
VFQHSEL
