CNPY2_HUMAN
ID CNPY2_HUMAN Reviewed; 182 AA.
AC Q9Y2B0; B2R7B9; Q9UHE9;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Protein canopy homolog 2;
DE AltName: Full=MIR-interacting saposin-like protein;
DE AltName: Full=Putative secreted protein Zsig9;
DE AltName: Full=Transmembrane protein 4;
DE Flags: Precursor;
GN Name=CNPY2; Synonyms=MSAP, TMEM4, ZSIG9; ORFNames=UNQ1943/PRO4426;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Gastric adenocarcinoma;
RX PubMed=10072769; DOI=10.1016/s0378-1119(99)00004-9;
RA Yokoyama-Kobayashi M., Yamaguchi T., Sekine S., Kato S.;
RT "Selection of cDNAs encoding putative type II membrane proteins on the cell
RT surface from a human full-length cDNA bank.";
RL Gene 228:161-167(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH MYLIP, AND TISSUE
RP SPECIFICITY.
RX PubMed=12826659; DOI=10.1074/jbc.m306271200;
RA Bornhauser B.C., Olsson P.-A., Lindholm D.;
RT "MSAP is a novel MIR-interacting protein that enhances neurite outgrowth
RT and increases myosin regulatory light chain.";
RL J. Biol. Chem. 278:35412-35420(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Sheppard P., Jelinek L., Whitmore T., Blumberg H., Lehner J., O'Hara P.;
RT "Homo sapiens putative secreted protein.";
RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Eye, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: Positive regulator of neurite outgrowth by stabilizing myosin
CC regulatory light chain (MRLC). It prevents MIR-mediated MRLC
CC ubiquitination and its subsequent proteasomal degradation.
CC -!- SUBUNIT: Interacts with MYLIP/MIR. {ECO:0000269|PubMed:12826659}.
CC -!- INTERACTION:
CC Q9Y2B0; P18825: ADRA2C; NbExp=3; IntAct=EBI-1054195, EBI-12015266;
CC Q9Y2B0; Q8WY64: MYLIP; NbExp=3; IntAct=EBI-1054195, EBI-6952711;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000255|PROSITE-
CC ProRule:PRU10138}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y2B0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y2B0-2; Sequence=VSP_010884, VSP_010885;
CC -!- TISSUE SPECIFICITY: Expressed in different tissues. Highest levels are
CC detected in adult placenta, liver and pancreas.
CC {ECO:0000269|PubMed:12826659}.
CC -!- SIMILARITY: Belongs to the canopy family. {ECO:0000305}.
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DR EMBL; AB015631; BAA76498.1; -; mRNA.
DR EMBL; AY032624; AAK38148.1; -; mRNA.
DR EMBL; AF186113; AAF01431.1; -; mRNA.
DR EMBL; AY359102; AAQ89460.1; -; mRNA.
DR EMBL; AK312921; BAG35766.1; -; mRNA.
DR EMBL; CH471054; EAW96926.1; -; Genomic_DNA.
DR EMBL; BC001027; AAH01027.1; -; mRNA.
DR EMBL; BC065015; AAH65015.1; -; mRNA.
DR CCDS; CCDS8914.1; -. [Q9Y2B0-1]
DR RefSeq; NP_001177920.1; NM_001190991.2. [Q9Y2B0-2]
DR RefSeq; NP_055070.1; NM_014255.6. [Q9Y2B0-1]
DR AlphaFoldDB; Q9Y2B0; -.
DR SMR; Q9Y2B0; -.
DR BioGRID; 115613; 40.
DR DIP; DIP-50705N; -.
DR IntAct; Q9Y2B0; 24.
DR MINT; Q9Y2B0; -.
DR STRING; 9606.ENSP00000273308; -.
DR GlyGen; Q9Y2B0; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9Y2B0; -.
DR PhosphoSitePlus; Q9Y2B0; -.
DR BioMuta; CNPY2; -.
DR DMDM; 50401205; -.
DR EPD; Q9Y2B0; -.
DR jPOST; Q9Y2B0; -.
DR MassIVE; Q9Y2B0; -.
DR MaxQB; Q9Y2B0; -.
DR PaxDb; Q9Y2B0; -.
DR PeptideAtlas; Q9Y2B0; -.
DR PRIDE; Q9Y2B0; -.
DR ProteomicsDB; 85715; -. [Q9Y2B0-1]
DR ProteomicsDB; 85716; -. [Q9Y2B0-2]
DR TopDownProteomics; Q9Y2B0-1; -. [Q9Y2B0-1]
DR TopDownProteomics; Q9Y2B0-2; -. [Q9Y2B0-2]
DR Antibodypedia; 50939; 194 antibodies from 26 providers.
DR DNASU; 10330; -.
DR Ensembl; ENST00000273308.9; ENSP00000273308.4; ENSG00000257727.6. [Q9Y2B0-1]
DR GeneID; 10330; -.
DR KEGG; hsa:10330; -.
DR MANE-Select; ENST00000273308.9; ENSP00000273308.4; NM_014255.7; NP_055070.1.
DR UCSC; uc001sku.3; human. [Q9Y2B0-1]
DR CTD; 10330; -.
DR DisGeNET; 10330; -.
DR GeneCards; CNPY2; -.
DR HGNC; HGNC:13529; CNPY2.
DR HPA; ENSG00000257727; Low tissue specificity.
DR MIM; 605861; gene.
DR neXtProt; NX_Q9Y2B0; -.
DR OpenTargets; ENSG00000257727; -.
DR PharmGKB; PA162382584; -.
DR VEuPathDB; HostDB:ENSG00000257727; -.
DR eggNOG; KOG3782; Eukaryota.
DR GeneTree; ENSGT00940000161158; -.
DR HOGENOM; CLU_095726_2_1_1; -.
DR InParanoid; Q9Y2B0; -.
DR OMA; VWARRSQ; -.
DR OrthoDB; 1417418at2759; -.
DR PhylomeDB; Q9Y2B0; -.
DR TreeFam; TF318578; -.
DR PathwayCommons; Q9Y2B0; -.
DR SignaLink; Q9Y2B0; -.
DR BioGRID-ORCS; 10330; 70 hits in 1080 CRISPR screens.
DR ChiTaRS; CNPY2; human.
DR GenomeRNAi; 10330; -.
DR Pharos; Q9Y2B0; Tbio.
DR PRO; PR:Q9Y2B0; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q9Y2B0; protein.
DR Bgee; ENSG00000257727; Expressed in adenohypophysis and 198 other tissues.
DR ExpressionAtlas; Q9Y2B0; baseline and differential.
DR Genevisible; Q9Y2B0; HS.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0010629; P:negative regulation of gene expression; ISS:BHF-UCL.
DR GO; GO:1905599; P:positive regulation of low-density lipoprotein receptor activity; ISS:BHF-UCL.
DR GO; GO:0010988; P:regulation of low-density lipoprotein particle clearance; ISS:BHF-UCL.
DR InterPro; IPR042415; CNPY.
DR InterPro; IPR021852; DUF3456.
DR InterPro; IPR008139; SaposinB_dom.
DR PANTHER; PTHR13341; PTHR13341; 1.
DR Pfam; PF11938; DUF3456; 1.
DR PROSITE; PS00014; ER_TARGET; 1.
DR PROSITE; PS50015; SAP_B; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Endoplasmic reticulum;
KW Phosphoprotein; Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..182
FT /note="Protein canopy homolog 2"
FT /id="PRO_0000031666"
FT DOMAIN 24..175
FT /note="Saposin B-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT MOTIF 179..182
FT /note="Prevents secretion from ER"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10138"
FT MOD_RES 115
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9QXT0"
FT DISULFID 28..171
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 31..164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 86..137
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT VAR_SEQ 70..84
FT /note="PYARSEAHLTELLEE -> TVTVPPNKVAHSGFG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.3"
FT /id="VSP_010884"
FT VAR_SEQ 85..182
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.3"
FT /id="VSP_010885"
SQ SEQUENCE 182 AA; 20652 MW; BE726D302490733F CRC64;
MKGWGWLALL LGALLGTAWA RRSQDLHCGA CRALVDELEW EIAQVDPKKT IQMGSFRINP
DGSQSVVEVP YARSEAHLTE LLEEICDRMK EYGEQIDPST HRKNYVRVVG RNGESSELDL
QGIRIDSDIS GTLKFACESI VEEYEDELIE FFSREADNVK DKLCSKRTDL CDHALHISHD
EL
