CNPY4_MOUSE
ID CNPY4_MOUSE Reviewed; 245 AA.
AC Q8BQ47; Q8BT84; Q8BTB1; Q8CCG0;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Protein canopy homolog 4;
DE AltName: Full=Protein associated with Tlr4;
DE Flags: Precursor;
GN Name=Cnpy4; Synonyms=Prat4b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Spinal ganglion, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH TLR4, AND TISSUE SPECIFICITY.
RX PubMed=16338228; DOI=10.1016/j.bbrc.2005.11.123;
RA Konno K., Wakabayashi Y., Akashi-Takamura S., Ishii T., Kobayashi M.,
RA Takahashi K., Kusumoto Y., Saitoh S., Yoshizawa Y., Miyake K.;
RT "A molecule that is associated with Toll-like receptor 4 and regulates its
RT cell surface expression.";
RL Biochem. Biophys. Res. Commun. 339:1076-1082(2006).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a role in the regulation of the cell surface expression
CC of TLR4. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with TLR4. {ECO:0000269|PubMed:16338228}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in lung, spleen, thymus, and
CC uterus. Moderately expressed in kidney, stomach and placenta. Weakly
CC expressed in brain, heart, liver, small intestine, skeletal muscle and
CC testis. {ECO:0000269|PubMed:16338228}.
CC -!- SIMILARITY: Belongs to the canopy family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC25336.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAC25383.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK011474; BAC25336.1; ALT_FRAME; mRNA.
DR EMBL; AK012968; BAC25383.1; ALT_FRAME; mRNA.
DR EMBL; AK033219; BAC28201.1; -; mRNA.
DR EMBL; AK051562; BAC34675.1; -; mRNA.
DR EMBL; BC086647; AAH86647.1; -; mRNA.
DR EMBL; BC094672; AAH94672.1; -; mRNA.
DR CCDS; CCDS39341.1; -.
DR RefSeq; NP_848727.1; NM_178612.4.
DR AlphaFoldDB; Q8BQ47; -.
DR BioGRID; 211486; 3.
DR IntAct; Q8BQ47; 1.
DR MINT; Q8BQ47; -.
DR STRING; 10090.ENSMUSP00000106559; -.
DR iPTMnet; Q8BQ47; -.
DR PhosphoSitePlus; Q8BQ47; -.
DR EPD; Q8BQ47; -.
DR jPOST; Q8BQ47; -.
DR MaxQB; Q8BQ47; -.
DR PaxDb; Q8BQ47; -.
DR PeptideAtlas; Q8BQ47; -.
DR PRIDE; Q8BQ47; -.
DR ProteomicsDB; 283582; -.
DR Antibodypedia; 2588; 53 antibodies from 18 providers.
DR DNASU; 66455; -.
DR Ensembl; ENSMUST00000110934; ENSMUSP00000106559; ENSMUSG00000036968.
DR GeneID; 66455; -.
DR KEGG; mmu:66455; -.
DR UCSC; uc009aez.1; mouse.
DR CTD; 245812; -.
DR MGI; MGI:1913705; Cnpy4.
DR VEuPathDB; HostDB:ENSMUSG00000036968; -.
DR eggNOG; KOG4052; Eukaryota.
DR GeneTree; ENSGT00390000014072; -.
DR HOGENOM; CLU_078068_0_1_1; -.
DR InParanoid; Q8BQ47; -.
DR OMA; TISDWYF; -.
DR OrthoDB; 1412562at2759; -.
DR PhylomeDB; Q8BQ47; -.
DR TreeFam; TF318951; -.
DR BioGRID-ORCS; 66455; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q8BQ47; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8BQ47; protein.
DR Bgee; ENSMUSG00000036968; Expressed in humerus cartilage element and 220 other tissues.
DR ExpressionAtlas; Q8BQ47; baseline and differential.
DR Genevisible; Q8BQ47; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005102; F:signaling receptor binding; IPI:MGI.
DR GO; GO:1903078; P:positive regulation of protein localization to plasma membrane; ISO:MGI.
DR GO; GO:0032880; P:regulation of protein localization; ISO:MGI.
DR InterPro; IPR021852; DUF3456.
DR Pfam; PF11938; DUF3456; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Disulfide bond; Reference proteome; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..245
FT /note="Protein canopy homolog 4"
FT /id="PRO_0000314019"
FT REGION 207..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 209..237
FT /evidence="ECO:0000255"
FT COMPBIAS 217..231
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 44..202
FT /evidence="ECO:0000250"
FT DISULFID 47..190
FT /evidence="ECO:0000250"
FT DISULFID 100..162
FT /evidence="ECO:0000250"
FT CONFLICT 22
FT /note="A -> V (in Ref. 1; BAC28201)"
FT /evidence="ECO:0000305"
FT CONFLICT 164
FT /note="T -> K (in Ref. 1; BAC25383)"
FT /evidence="ECO:0000305"
FT CONFLICT 219..222
FT /note="EADD -> APDH (in Ref. 1; BAC25383)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 245 AA; 28094 MW; DAFAB8F191A34029 CRC64;
MCGLRFIMGP VRLEILLFIL AAYGAWAEAT KEEEDDTERL PSKCEVCKLL SMELQEALSR
TGRSREVLEL GQVLDTGKRK RHVPYSLSET RLEEALENLC ERILDYNVHA ERKGSLRYAK
GQSQTMATLK GLVQKGVKVD LGIPLELWDE PSVEVTFLKK QCETMLEEFE DVVGDWYFHH
QEQPLQHFLC ERHVLPASET ACLREAWTGK EKISDGQEEA DDEEEEEEEE ITKTSGNPKH
DPEDL
