CNR14_CAEEL
ID CNR14_CAEEL Reviewed; 534 AA.
AC P41830;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Steroid hormone receptor family member cnr14;
DE AltName: Full=Nuclear receptor subfamily 1 group G member 1;
GN Name=sex-1 {ECO:0000312|WormBase:F44A6.2};
GN Synonyms=cnr-14 {ECO:0000312|WormBase:F44A6.2},
GN nhr-24 {ECO:0000312|WormBase:F44A6.2},
GN nr1g1 {ECO:0000312|WormBase:F44A6.2};
GN ORFNames=F44A6.2 {ECO:0000312|WormBase:F44A6.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Bristol N2;
RX PubMed=7816808; DOI=10.1073/pnas.92.1.156;
RA Kostrouch Z., Kostrouchova M., Rall J.E.;
RT "Steroid/thyroid hormone receptor genes in Caenorhabditis elegans.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:156-159(1995).
RN [2]
RP SEQUENCE REVISION TO C-TERMINUS.
RA Kostrouchova M.;
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION.
RX PubMed=16139225; DOI=10.1016/j.devcel.2005.06.009;
RA Powell J.R., Jow M.M., Meyer B.J.;
RT "The T-box transcription factor SEA-1 is an autosomal element of the X:A
RT signal that determines C. elegans sex.";
RL Dev. Cell 9:339-349(2005).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17720939; DOI=10.1534/genetics.106.061812;
RA Gladden J.M., Meyer B.J.;
RT "A ONECUT homeodomain protein communicates X chromosome dose to specify
RT Caenorhabditis elegans sexual fate by repressing a sex switch gene.";
RL Genetics 177:1621-1637(2007).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21471153; DOI=10.1242/dev.057109;
RA Huang X., Zhang H., Zhang H.;
RT "The zinc-finger protein SEA-2 regulates larval developmental timing and
RT adult lifespan in C. elegans.";
RL Development 138:2059-2068(2011).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=23666922; DOI=10.1101/gad.217026.113;
RA Farboud B., Nix P., Jow M.M., Gladden J.M., Meyer B.J.;
RT "Molecular antagonism between X-chromosome and autosome signals determines
RT nematode sex.";
RL Genes Dev. 27:1159-1178(2013).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=33372658; DOI=10.7554/elife.62963;
RA Farboud B., Novak C.S., Nicoll M., Quiogue A., Meyer B.J.;
RT "Dose-dependent action of the RNA binding protein FOX-1 to relay X-
RT chromosome number and determine C. elegans sex.";
RL Elife 9:0-0(2020).
CC -!- FUNCTION: Transciptional regulator which is involved in the sex
CC determination and X chromosome dosage compensation pathways
CC (PubMed:21471153, PubMed:23666922, PubMed:16139225, PubMed:17720939,
CC PubMed:33372658). Directly binds to five 5'-A(G/C)(G/T)(T/G)C(A/G)-3'
CC sites in the promoter of sex-determining factor xol-1 to negatively
CC regulate its expression and promote hermaphrodite development
CC (PubMed:23666922). Together with fox-1 is involved in making the
CC distinction between one and two X-chromosomes (PubMed:21471153,
CC PubMed:23666922, PubMed:33372658). Plays a role in the fox-1-mediated
CC repression of the functionally active isoform (isoform b) of the sex-
CC determining factor xol-1 gene to promote hermaphrodite development
CC (PubMed:33372658). Plays a role in the association of the dosage
CC compensation complex proteins dpy-27 and sdc-3 with the hermaphrodite X
CC chromosomes (PubMed:16139225, PubMed:17720939).
CC {ECO:0000269|PubMed:16139225, ECO:0000269|PubMed:17720939,
CC ECO:0000269|PubMed:21471153, ECO:0000269|PubMed:23666922,
CC ECO:0000269|PubMed:33372658}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:23666922}.
CC -!- TISSUE SPECIFICITY: Most abundant in embryos.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in 43% lethality
CC of hermaphrodites (PubMed:33372658). RNAi-mediated knockdown together
CC with fox-1 results in hermaphrodite embryonic lethality
CC (PubMed:21471153, PubMed:33372658). This hermaphrodite-specific
CC lethality is suppressed in a sea-2 bp283 mutant or sea-1 gk799 mutant
CC background (PubMed:21471153). RNAi-mediated knockdown results in
CC hermaphrodites lethality due to failure of the dosage compensation
CC complex to assemble on X chromosomes in a fox-1 y303 mutant background
CC (PubMed:17720939). RNAi-mediated knockdown in a background containing
CC one copy of the fox-1 gene results in the viability of 3% of
CC hermaphrodites (PubMed:33372658). RNAi-mediated knockdown in a strain
CC where all of the GCACG and GCUAG motifs in intron 6 of the xol-1 gene
CC have been mutated to AUACA and AUAUA, respectively results in the
CC viability of 1% of hermaphrodites (PubMed:33372658). RNAi-mediated
CC knockdown in a strain where all of the GCACG and GCUAG motifs in intron
CC 6 of one copy of the xol-1 gene have been mutated to AUACA and AUAUA,
CC respectively results in the viability of 7% of hermaphrodites
CC (PubMed:33372658). RNAi-mediated knockdown in a strain where all of the
CC GCACG motifs in intron 6 of the xol-1 gene have been mutated to AUACA
CC results in the viability of 24% of hermaphrodites (PubMed:33372658).
CC RNAi-mediated knockdown in a strain where all of the GCACG motifs in
CC intron 6 of one copy of the xol-1 gene have been mutated to AUACA
CC results in the viability of 18% of hermaphrodites (PubMed:33372658).
CC RNAi-mediated knockdown in a strain where all of the GCUAG motifs in
CC intron 6 of the xol-1 gene have been mutated to AUAUA results in the
CC viability of 33% of hermaphrodites (PubMed:33372658). RNAi-mediated
CC knockdown in a strain where all of the GCUAG motifs in intron 6 of one
CC copy of the xol-1 gene have been mutated to AUAUA results in the
CC viability of 13% of hermaphrodites (PubMed:33372658). RNAi-mediated
CC knockdown in a strain where all of the GCACG and GCUAG motifs in intron
CC 6 of the xol-1 gene have been mutated to GCUUG results in the viability
CC of 8% of hermaphrodites (PubMed:33372658). RNAi-mediated knockdown in a
CC strain where all of the GCACG and GCUAG motifs in intron 6 of one copy
CC of the xol-1 gene have been mutated to GCUUG results in the viability
CC of 36% of hermaphrodites (PubMed:33372658). RNAi-mediated knockdown in
CC strains containing five or three fox-1-binding GCACG motifs in intron 6
CC of the xol-1 gene results in the viability of 49% and 33% of
CC hermaphrodites, respectively (PubMed:33372658).
CC {ECO:0000269|PubMed:17720939, ECO:0000269|PubMed:21471153,
CC ECO:0000269|PubMed:33372658}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR1
CC subfamily. {ECO:0000305}.
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DR EMBL; U13074; AAA96982.1; -; mRNA.
DR EMBL; BX284606; CAA90722.1; -; Genomic_DNA.
DR PIR; T22154; T22154.
DR RefSeq; NP_001024662.1; NM_001029491.2.
DR AlphaFoldDB; P41830; -.
DR SMR; P41830; -.
DR BioGRID; 46145; 30.
DR DIP; DIP-24967N; -.
DR IntAct; P41830; 15.
DR STRING; 6239.F44A6.2.1; -.
DR EPD; P41830; -.
DR PaxDb; P41830; -.
DR PeptideAtlas; P41830; -.
DR PRIDE; P41830; -.
DR EnsemblMetazoa; F44A6.2.1; F44A6.2.1; WBGene00004786.
DR GeneID; 181231; -.
DR KEGG; cel:CELE_F44A6.2; -.
DR UCSC; F44A6.2.1; c. elegans.
DR CTD; 181231; -.
DR WormBase; F44A6.2; CE03323; WBGene00004786; sex-1.
DR eggNOG; KOG4846; Eukaryota.
DR GeneTree; ENSGT00940000174451; -.
DR HOGENOM; CLU_040350_0_0_1; -.
DR InParanoid; P41830; -.
DR OMA; FANHIIT; -.
DR OrthoDB; 1240230at2759; -.
DR PhylomeDB; P41830; -.
DR SignaLink; P41830; -.
DR PRO; PR:P41830; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00004786; Expressed in embryo and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:WormBase.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0042464; P:dosage compensation by hypoactivation of X chromosome; IMP:UniProtKB.
DR GO; GO:0009755; P:hormone-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007538; P:primary sex determination; IMP:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:UniProtKB.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 2: Evidence at transcript level;
KW Differentiation; DNA-binding; Metal-binding; Nucleus; Receptor;
KW Reference proteome; Sexual differentiation; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..534
FT /note="Steroid hormone receptor family member cnr14"
FT /id="PRO_0000053523"
FT DOMAIN 252..493
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 148..223
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 151..171
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 187..211
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 30..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 502..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 534 AA; 60848 MW; E6E888F18750E1B0 CRC64;
MSFETKPNYL LLTNPDTPLS VCTSPYYSPS GKTASIPSSE ASKPEGTNGQ WSHLPTGATY
VTDEFSSEFQ IQNGSTAAQS GNANNYADPL SHRRYFNNVN GYNHHQFYDT ASQASVSSPA
TSVTSSLSPP DSLSNGHTTQ RHHIGKAISF CKVCGDKASG YHYGVTSCEG CKGFFRRSIQ
RKIDYRCLKQ QVCEIKRESR NRCQYCRFKK CLDSGMSKDS VRQMKFRNAM RDDKSPDSVF
VPEISTLERQ EEVDAVYEAV LRAHTTFSFY TDIKIRSIVA RPFNVRINED SKMNRLNAWQ
IYAHEIDVDI KEVVNFVKEI PKFNFINGND KAVLLRKNAF PLYLLRIVRG MSNRGLMLRD
GRLIDFKSLQ LLYGSLADEM LAFANHIITI GCTDGDIALF IVLILCQPLT TEQQFSTNFK
SQLQLLEMFD FYKKVLFQKM TCRIDGCDTY KQLMKCIHEL NRLNELHKQQ LNILRENLSF
LNLPPLVVEM FQLSTLPLPV NHNNQENQYT PAPEHQSPQP QQPTPNQQQT PVHC
