CNRA_CUPMC
ID CNRA_CUPMC Reviewed; 1076 AA.
AC P37972; Q5NUX5;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 2.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Nickel and cobalt resistance protein CnrA;
GN Name=cnrA; OrderedLocusNames=Rmet_6210; ORFNames=RMe0083;
OS Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS CH34) (Ralstonia metallidurans).
OG Plasmid pMOL28.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=266264;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8380802; DOI=10.1128/jb.175.3.767-778.1993;
RA Liesegang H., Lemke K., Siddiqui R.A., Schlegel H.-G.;
RT "Characterization of the inducible nickel and cobalt resistance determinant
RT cnr from pMOL28 of Alcaligenes eutrophus CH34.";
RL J. Bacteriol. 175:767-778(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA van der Lelie D., Monchy S., Taghavi S., McCorkle S., Dunn J.,
RA Benotmane M., Vallaeys T., Lapidus A., Mergeay M.;
RT "Sequence and features of the Ralstonia metallidurans CH34 heavy metal
RT plasmids pMOL28 and pMOL30.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT master survivalist in harsh and anthropogenic environments.";
RL PLoS ONE 5:E10433-E10433(2010).
RN [4]
RP INDUCTION.
RX PubMed=10671463; DOI=10.1128/jb.182.5.1390-1398.2000;
RA Grass G., Grosse C., Nies D.H.;
RT "Regulation of the cnr cobalt and nickel resistance determinant from
RT Ralstonia sp. strain CH34.";
RL J. Bacteriol. 182:1390-1398(2000).
CC -!- FUNCTION: The products of the genes cnrA, cnrB, and cnrC are likely to
CC form a membrane-bound protein complex catalyzing an energy-dependent
CC efflux of Ni(2+) and Co(2+). The mechanism of action of the CnrCBA
CC complex may be that of a proton/cation antiporter.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- INDUCTION: By nickel. {ECO:0000269|PubMed:10671463}.
CC -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC (TC 2.A.6) family. {ECO:0000305}.
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DR EMBL; M91650; AAA21970.1; -; Genomic_DNA.
DR EMBL; X90708; CAI30227.1; -; Genomic_DNA.
DR EMBL; CP000355; ABF13069.1; -; Genomic_DNA.
DR RefSeq; WP_011239966.1; NC_007972.2.
DR RefSeq; YP_161705.1; NC_006525.1.
DR AlphaFoldDB; P37972; -.
DR SMR; P37972; -.
DR TCDB; 2.A.6.1.1; the resistance-nodulation-cell division (rnd) superfamily.
DR EnsemblBacteria; ABF13069; ABF13069; Rmet_6210.
DR KEGG; rme:Rmet_6210; -.
DR HOGENOM; CLU_002755_1_2_4; -.
DR OMA; WIIAPQM; -.
DR OrthoDB; 91817at2; -.
DR Proteomes; UP000002429; Plasmid pMOL28.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 3.30.2090.10; -; 2.
DR InterPro; IPR027463; AcrB_DN_DC_subdom.
DR InterPro; IPR001036; Acrflvin-R.
DR InterPro; IPR004763; CzcA/CusA/SilA/NccA/HelA/CnrA.
DR PANTHER; PTHR32063; PTHR32063; 1.
DR Pfam; PF00873; ACR_tran; 1.
DR PRINTS; PR00702; ACRIFLAVINRP.
DR SUPFAM; SSF82714; SSF82714; 2.
DR TIGRFAMs; TIGR00914; 2A0601; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Cobalt; Membrane; Nickel; Plasmid;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1076
FT /note="Nickel and cobalt resistance protein CnrA"
FT /id="PRO_0000161814"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 475..495
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 502..522
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 561..581
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 903..923
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 928..948
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 959..979
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1003..1023
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1035..1055
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 108..109
FT /note="QQ -> HE (in Ref. 1; AAA21970)"
FT /evidence="ECO:0000305"
FT CONFLICT 281..288
FT /note="QRQNVPIT -> HGKMSHH (in Ref. 1; AAA21970)"
FT /evidence="ECO:0000305"
FT CONFLICT 460
FT /note="E -> D (in Ref. 1; AAA21970)"
FT /evidence="ECO:0000305"
FT CONFLICT 490
FT /note="C -> S (in Ref. 1; AAA21970)"
FT /evidence="ECO:0000305"
FT CONFLICT 936
FT /note="V -> S (in Ref. 1; AAA21970)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1076 AA; 115738 MW; EC9AB46BAA3CF46D CRC64;
MIESILSGSV RYRWLVLFLT AVVAVIGAWQ LNLLPIDVTP DITNKQVQIN SVVPTMSPVE
VEKRVTYPIE TAIAGLNGVE STRSMSRNGF SQVTVIFKES ANLYFMRQQV SERLAQARPN
LPENVEPQMG PVSTGLGEVF HYSVEYQYPD GTGASIKDGE PGWQSDGSFL TERGERLDDR
VSRLAYLRTV QDWIIRPQLR TTPGVADVDS LGGYVKQFVV EPDTGKMAAY GVSYADLARA
LEDTNLSVGA NFIRRSGESY LVRADARIKS ADEISRAVIA QRQNVPITVG QVARVKIGGE
LRSGAASRNG NETVVGSALM LVGANSRTVA QAVGDKLEQI SKTLPPGVVI VPTLNRSQLV
IATIETVAKN LIEGALLVVA ILFALLGNWR AATIAALVIP LSLLVSAIGM NQFHISGNLM
SLGALDFGLI IDGAVIIVEN SLRRLAERQH REGRLLTLDE RLQEVVQSSR EMVRPTVYGQ
LVIFMVFLPC LTFQGVEGKM FSPMVITLML ALASAFVLSL TFVPAMVAVM LRKKVAETEV
RVIVATKESY RPWLEHAVAR PMPFIGAGIA TVAVATVAFT FVGREFMPTL DELNLNLSSV
RIPSTSIDQS VAIDLPLERA VLSLPEVQTV YSKAGTASLA ADPMPPNASD NYIILKPKSE
WPEGVTTKEQ VIERIREKTA PMVGNNYDVT QPIEMRFNEL IGGVRSDVAV KVYGENLDEL
AATAQRIAAV LKKTPGATDV RVPLTSGFPT FDIVFDRAAI ARYGLTVKEV ADTISTAMAG
RPAGQIFDGD RRFDIVIRLP GEQRENLDVL GALPVMLPLS EGQARASVPL RQLVQFRFTQ
GLNEVSRDNG KRRVYVEANV GGRDLGSFVD DAAARIAKEV KLPPGMYIEW GGQFQNLQAA
TKRLAIIVPL CFILIAATLY MAIGSAALTA TVLTAVPLAL AGGVFALLLR GIPFSISAAV
GFIAVSGVAV LNGLVLISAI RKRLDDGMAP DAAVIEGAME RVRPVLMTAL VASLGFVPMA
IATGTGAEVQ KPLATVVIGG LVTATVLTLF VLPALCGIVL KRRTAGRPEA QAALEA
