ID   CNRB_CUPMC              Reviewed;         395 AA.
AC   P37973; Q5NUX4; Q7B055;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Nickel and cobalt resistance protein CnrB;
GN   Name=cnrB; OrderedLocusNames=Rmet_6209; ORFNames=RMe0084;
OS   Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS   CH34) (Ralstonia metallidurans).
OG   Plasmid pMOL28.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=266264;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8380802; DOI=10.1128/jb.175.3.767-778.1993;
RA   Liesegang H., Lemke K., Siddiqui R.A., Schlegel H.-G.;
RT   "Characterization of the inducible nickel and cobalt resistance determinant
RT   cnr from pMOL28 of Alcaligenes eutrophus CH34.";
RL   J. Bacteriol. 175:767-778(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   van der Lelie D., Monchy S., Taghavi S., McCorkle S., Dunn J.,
RA   Benotmane M., Vallaeys T., Lapidus A., Mergeay M.;
RT   "Sequence and features of the Ralstonia metallidurans CH34 heavy metal
RT   plasmids pMOL28 and pMOL30.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX   PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA   Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA   Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA   Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT   "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT   master survivalist in harsh and anthropogenic environments.";
RL   PLoS ONE 5:E10433-E10433(2010).
RN   [4]
RP   INDUCTION.
RX   PubMed=10671463; DOI=10.1128/jb.182.5.1390-1398.2000;
RA   Grass G., Grosse C., Nies D.H.;
RT   "Regulation of the cnr cobalt and nickel resistance determinant from
RT   Ralstonia sp. strain CH34.";
RL   J. Bacteriol. 182:1390-1398(2000).
CC   -!- FUNCTION: The products of the genes cnrA, cnrB, and cnrC are likely to
CC       form a membrane-bound protein complex catalyzing an energy-dependent
CC       efflux of Ni(2+) and Co(2+). The mechanism of action of the CnrCBA
CC       complex may be that of a proton/cation antiporter.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: By nickel. {ECO:0000269|PubMed:10671463}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; M91650; AAA21969.1; -; Genomic_DNA.
DR   EMBL; AJ276513; CAB82452.1; -; Genomic_DNA.
DR   EMBL; X90708; CAI30228.1; -; Genomic_DNA.
DR   EMBL; CP000355; ABF13068.1; -; Genomic_DNA.
DR   PIR; F47056; F47056.
DR   RefSeq; WP_011239967.1; NC_007972.2.
DR   RefSeq; YP_161706.1; NC_006525.1.
DR   AlphaFoldDB; P37973; -.
DR   SMR; P37973; -.
DR   TCDB; 8.A.1.2.1; the membrane fusion protein (mfp) family.
DR   EnsemblBacteria; ABF13068; ABF13068; Rmet_6209.
DR   GeneID; 60825773; -.
DR   KEGG; rme:Rmet_6209; -.
DR   HOGENOM; CLU_018816_13_0_4; -.
DR   OMA; AGDYCRE; -.
DR   OrthoDB; 1174564at2; -.
DR   Proteomes; UP000002429; Plasmid pMOL28.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046873; F:metal ion transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR005695; Co/Zn/Cd_resistance_CzcB.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR00999; 8a0102; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Cobalt; Coiled coil; Membrane; Nickel;
KW   Plasmid; Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..395
FT                   /note="Nickel and cobalt resistance protein CnrB"
FT                   /id="PRO_0000201863"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          35..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          140..193
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   395 AA;  40083 MW;  075C2165EB5BA09F CRC64;
     MMKNERRSVN WPMIAGVAAV AAAVGFGAAH LPVSEKSPAS TQAPEAQKPQ SAPVKPGLKE
     VKIPATYLAA ANIAVEPVAS AAVGTEILAP ATVAALPGSE AVIVSRAAGA VQRVQRRLGD
     VVKAGDVLAL VDSPEAAGMA AERKVAQAKA DLARKTYERE ASLFQQGVTP RQEMEAAKAA
     LDVAQAEALR AATVAQSAHL ASDGRSVAVV SPIAGKITAQ SVTLGAFVAP QAELFRVAGT
     GAVQVEAAVT AADTSRIVAG SEATILLANG SPLSARVQAV TPTVTGSARV ATVVVVPAQP
     TDRLVVGEGV QVRLRTAVAD AAALSVPEDA VQNLDGRDVL FVRTQEGFRP MPVLVGTRSG
     GSAQILSGVQ AGEQVATRNA FLVKAEMNKG GGDEE