ID   CNRC_CUPMC              Reviewed;         418 AA.
AC   P37974; Q5NUX3; Q7B056;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Nickel and cobalt resistance protein CnrC;
DE   Flags: Precursor;
GN   Name=cnrC; OrderedLocusNames=Rmet_6208; ORFNames=RMe0085;
OS   Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS   CH34) (Ralstonia metallidurans).
OG   Plasmid pMOL28.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=266264;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8380802; DOI=10.1128/jb.175.3.767-778.1993;
RA   Liesegang H., Lemke K., Siddiqui R.A., Schlegel H.-G.;
RT   "Characterization of the inducible nickel and cobalt resistance determinant
RT   cnr from pMOL28 of Alcaligenes eutrophus CH34.";
RL   J. Bacteriol. 175:767-778(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   van der Lelie D., Monchy S., Taghavi S., McCorkle S., Dunn J.,
RA   Benotmane M., Vallaeys T., Lapidus A., Mergeay M.;
RT   "Sequence and features of the Ralstonia metallidurans CH34 heavy metal
RT   plasmids pMOL28 and pMOL30.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX   PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA   Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA   Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA   Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT   "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT   master survivalist in harsh and anthropogenic environments.";
RL   PLoS ONE 5:E10433-E10433(2010).
RN   [4]
RP   INDUCTION.
RX   PubMed=10671463; DOI=10.1128/jb.182.5.1390-1398.2000;
RA   Grass G., Grosse C., Nies D.H.;
RT   "Regulation of the cnr cobalt and nickel resistance determinant from
RT   Ralstonia sp. strain CH34.";
RL   J. Bacteriol. 182:1390-1398(2000).
CC   -!- FUNCTION: The products of the genes cnrA, cnrB, and cnrC are likely to
CC       form a membrane-bound protein complex catalyzing an energy-dependent
CC       efflux of Ni(2+) and Co(2+). The mechanism of action of the CnrCBA
CC       complex may be that of a proton/cation antiporter.
CC   -!- INDUCTION: By nickel. {ECO:0000269|PubMed:10671463}.
CC   -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M91650; AAA21968.1; -; Genomic_DNA.
DR   EMBL; AJ276513; CAB82451.1; -; Genomic_DNA.
DR   EMBL; X90708; CAI30229.1; -; Genomic_DNA.
DR   EMBL; CP000355; ABF13067.1; -; Genomic_DNA.
DR   PIR; E47056; E47056.
DR   RefSeq; WP_011239968.1; NC_007972.2.
DR   RefSeq; YP_161707.1; NC_006525.1.
DR   AlphaFoldDB; P37974; -.
DR   SMR; P37974; -.
DR   TCDB; 1.B.17.2.1; the outer membrane factor (omf) family.
DR   EnsemblBacteria; ABF13067; ABF13067; Rmet_6208.
DR   GeneID; 60825774; -.
DR   KEGG; rme:Rmet_6208; -.
DR   HOGENOM; CLU_012817_14_2_4; -.
DR   OMA; HIEIQRL; -.
DR   OrthoDB; 1533572at2; -.
DR   Proteomes; UP000002429; Plasmid pMOL28.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR003423; OMP_efflux.
DR   Pfam; PF02321; OEP; 2.
PE   2: Evidence at transcript level;
KW   Cobalt; Nickel; Plasmid; Reference proteome; Signal; Transport.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..418
FT                   /note="Nickel and cobalt resistance protein CnrC"
FT                   /id="PRO_0000020952"
SQ   SEQUENCE   418 AA;  43760 MW;  C7A277D975CE69E5 CRC64;
     MKQVISSFLC RPRFVGSAIW LLPVALSHAA EAPPFPNLLQ QSLALAPAMV AQAANVRAAG
     ADAAQAQAWL NPRIDTVLEN LGAPSSDGLS QRQNTYSITQ PFELGGKRGA RIEVGERNFA
     AAQARERQAQ VAYAAELAVA YATAEAALGR KILATENLAR ANEELAAARA LVDSGKEASL
     RSAQAKASVA AAQAAEAAAT NDATQALARL SAMSGASEPY TAVTSSLLTT QAVVPNAPAA
     LAESPSVRAA EAERNALDAQ VDVERKRWIP DVGVSAGVRR YGWTNSSGYV VGVTASIPLF
     DQNRNGINAA VERVAAAQAR LDSVRLEANV ARQSAISQVA TADKQLAAAS EGEQAAAEAY
     RMGRIGYESG KTPLMELLAV RRALVDARQL TIDARLARVR ALAALAQADG RLAFEESR