CNRG_BOVIN
ID CNRG_BOVIN Reviewed; 87 AA.
AC P04972;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma;
DE Short=GMP-PDE gamma;
DE EC=3.1.4.35;
GN Name=PDE6G; Synonyms=PDEG;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP PROTEIN SEQUENCE, AND ACETYLATION AT MET-1.
RC TISSUE=Retina;
RX PubMed=3011376;
RA Ovchinnikov Y.A., Muradov K.G., Feigina M.Y., Nazimov I.V.,
RA Khoroshilova N.I.;
RT "Cyclic GMP-phosphodiesterase from the cattle retina. Amino acid sequence
RT of the gamma subunit.";
RL Dokl. Akad. Nauk SSSR 287:1496-1498(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Retina;
RA Ovchinnikov Y.A., Gubanov V.V., Khramtsov N.V., Akhmedov N.B.,
RA Zagranichny V.E., Ishchenko K.A., Muradov K.G., Barinov A.A.,
RA Bondarenko V.A., Kumarev V.P., Kobzev V.F., Lipkin V.M.;
RT "Cyclic GMP phosphodiesterase from bovine retina. Nucleotide sequence of
RT cDNA of the gamma-subunit.";
RL Dokl. Biochem. 288:216-218(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Retina;
RX PubMed=3015681; DOI=10.1016/0014-5793(86)80830-4;
RA Ovchinnikov Y.A., Lipkin V.M., Kumarev V.P., Gubanov V.V., Khramtsov N.V.,
RA Akhmedov N.B., Zagranichny V.E., Muradov K.G.;
RT "Cyclic GMP phosphodiesterase from cattle retina. Amino acid sequence of
RT the gamma-subunit and nucleotide sequence of the corresponding cDNA.";
RL FEBS Lett. 204:288-292(1986).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 46-87 IN COMPLEX WITH RGS9 AND
RP GNAT1.
RX PubMed=11234020; DOI=10.1038/35059138;
RA Slep K.C., Kercher M.A., He W., Cowan C.W., Wensel T.G., Sigler P.B.;
RT "Structural determinants for regulation of phosphodiesterase by a G protein
RT at 2.0 A.";
RL Nature 409:1071-1077(2001).
CC -!- FUNCTION: Participates in processes of transmission and amplification
CC of the visual signal. cGMP-PDEs are the effector molecules in G-
CC protein-mediated phototransduction in vertebrate rods and cones.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3',5'-cyclic GMP + H2O = GMP + H(+); Xref=Rhea:RHEA:16957,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57746,
CC ChEBI:CHEBI:58115; EC=3.1.4.35;
CC -!- SUBUNIT: Oligomer composed of two catalytic chains (alpha and beta), an
CC inhibitory chain (gamma) and the delta chain.
CC {ECO:0000269|PubMed:11234020}.
CC -!- SIMILARITY: Belongs to the rod/cone cGMP-PDE gamma subunit family.
CC {ECO:0000305}.
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DR EMBL; X04823; CAA28507.1; -; mRNA.
DR EMBL; X04270; CAA27821.1; -; mRNA.
DR PIR; A25268; A25268.
DR RefSeq; NP_776846.1; NM_174421.2.
DR RefSeq; XP_010814750.1; XM_010816448.2.
DR RefSeq; XP_010814751.1; XM_010816449.1.
DR PDB; 1FQJ; X-ray; 2.02 A; C=46-87.
DR PDB; 2JU4; NMR; -; A=1-87.
DR PDB; 3JAB; EM; 11.00 A; D/P=70-87.
DR PDB; 3JBQ; EM; 11.00 A; D/X=70-87.
DR PDB; 6MZB; EM; 3.40 A; C/D=1-87.
DR PDB; 7JSN; EM; 3.20 A; C/D=1-87.
DR PDBsum; 1FQJ; -.
DR PDBsum; 2JU4; -.
DR PDBsum; 3JAB; -.
DR PDBsum; 3JBQ; -.
DR PDBsum; 6MZB; -.
DR PDBsum; 7JSN; -.
DR AlphaFoldDB; P04972; -.
DR BMRB; P04972; -.
DR SMR; P04972; -.
DR IntAct; P04972; 2.
DR MINT; P04972; -.
DR STRING; 9913.ENSBTAP00000000459; -.
DR BindingDB; P04972; -.
DR ChEMBL; CHEMBL2096979; -.
DR DrugCentral; P04972; -.
DR iPTMnet; P04972; -.
DR PaxDb; P04972; -.
DR PRIDE; P04972; -.
DR ABCD; P04972; 1 sequenced antibody.
DR Ensembl; ENSBTAT00000000459; ENSBTAP00000000459; ENSBTAG00000000354.
DR GeneID; 281977; -.
DR KEGG; bta:281977; -.
DR CTD; 5148; -.
DR VEuPathDB; HostDB:ENSBTAG00000000354; -.
DR VGNC; VGNC:32683; PDE6G.
DR eggNOG; ENOG502S20G; Eukaryota.
DR GeneTree; ENSGT00390000013260; -.
DR HOGENOM; CLU_170469_0_0_1; -.
DR InParanoid; P04972; -.
DR OMA; KGRGWHP; -.
DR OrthoDB; 1574918at2759; -.
DR TreeFam; TF333297; -.
DR EvolutionaryTrace; P04972; -.
DR PRO; PR:P04972; -.
DR Proteomes; UP000009136; Chromosome 19.
DR Bgee; ENSBTAG00000000354; Expressed in retina and 30 other tissues.
DR GO; GO:0097381; C:photoreceptor disc membrane; TAS:Reactome.
DR GO; GO:0042622; C:photoreceptor outer segment membrane; IDA:CAFA.
DR GO; GO:0047555; F:3',5'-cyclic-GMP phosphodiesterase activity; IEA:UniProtKB-EC.
DR GO; GO:0030553; F:cGMP binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IMP:CAFA.
DR GO; GO:0045742; P:positive regulation of epidermal growth factor receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0045745; P:positive regulation of G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; IEA:Ensembl.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR DisProt; DP00347; -.
DR Gene3D; 4.10.1120.10; -; 1.
DR InterPro; IPR006952; PDE6_gamma.
DR InterPro; IPR037030; PDE6_gamma_sf.
DR PANTHER; PTHR12122; PTHR12122; 1.
DR Pfam; PF04868; PDE6_gamma; 1.
DR PIRSF; PIRSF000969; 35-cGMP_Pdiase_g; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; cGMP; Direct protein sequencing; Hydrolase;
KW Reference proteome; Sensory transduction; Vision.
FT CHAIN 1..87
FT /note="Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic
FT phosphodiesterase subunit gamma"
FT /id="PRO_0000166113"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000269|PubMed:3011376"
FT TURN 12..14
FT /evidence="ECO:0007829|PDB:7JSN"
FT STRAND 24..27
FT /evidence="ECO:0007829|PDB:2JU4"
FT STRAND 36..38
FT /evidence="ECO:0007829|PDB:6MZB"
FT STRAND 45..48
FT /evidence="ECO:0007829|PDB:2JU4"
FT STRAND 55..58
FT /evidence="ECO:0007829|PDB:7JSN"
FT HELIX 62..65
FT /evidence="ECO:0007829|PDB:1FQJ"
FT HELIX 69..73
FT /evidence="ECO:0007829|PDB:1FQJ"
FT HELIX 78..83
FT /evidence="ECO:0007829|PDB:1FQJ"
SQ SEQUENCE 87 AA; 9669 MW; 3AA2D6B5D7D14D58 CRC64;
MNLEPPKAEI RSATRVMGGP VTPRKGPPKF KQRQTRQFKS KPPKKGVQGF GDDIPGMEGL
GTDITVICPW EAFNHLELHE LAQYGII
