CNRH_CUPMC
ID CNRH_CUPMC Reviewed; 191 AA.
AC P37978; P37976; Q5NUX2; Q7B057;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=RNA polymerase sigma factor CnrH;
GN Name=cnrH; OrderedLocusNames=Rmet_6207; ORFNames=RMe0086;
OS Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS CH34) (Ralstonia metallidurans).
OG Plasmid pMOL28.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=266264;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8380802; DOI=10.1128/jb.175.3.767-778.1993;
RA Liesegang H., Lemke K., Siddiqui R.A., Schlegel H.-G.;
RT "Characterization of the inducible nickel and cobalt resistance determinant
RT cnr from pMOL28 of Alcaligenes eutrophus CH34.";
RL J. Bacteriol. 175:767-778(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA van der Lelie D., Monchy S., Taghavi S., McCorkle S., Dunn J.,
RA Benotmane M., Vallaeys T., Lapidus A., Mergeay M.;
RT "Sequence and features of the Ralstonia metallidurans CH34 heavy metal
RT plasmids pMOL28 and pMOL30.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT master survivalist in harsh and anthropogenic environments.";
RL PLoS ONE 5:E10433-E10433(2010).
RN [4]
RP IDENTIFICATION, AND SIMILARITY TO OTHER ECF SIGMA FACTORS.
RX PubMed=8052622; DOI=10.1073/pnas.91.16.7573;
RA Lonetto M.A., Brown K.L., Rudd K.E., Buttner M.J.;
RT "Analysis of the Streptomyces coelicolor sigE gene reveals the existence of
RT a subfamily of eubacterial RNA polymerase sigma factors involved in the
RT regulation of extracytoplasmic functions.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:7573-7577(1994).
RN [5]
RP CHARACTERIZATION, AND INDUCTION.
RX PubMed=10671463; DOI=10.1128/jb.182.5.1390-1398.2000;
RA Grass G., Grosse C., Nies D.H.;
RT "Regulation of the cnr cobalt and nickel resistance determinant from
RT Ralstonia sp. strain CH34.";
RL J. Bacteriol. 182:1390-1398(2000).
RN [6]
RP DNA-BINDING, AND INDUCTION.
RX PubMed=10671464; DOI=10.1128/jb.182.5.1399-1409.2000;
RA Tibazarwa C., Wuertz S., Mergeay M., Wyns L., van der Lelie D.;
RT "Regulation of the cnr cobalt and nickel resistance determinant of
RT Ralstonia eutropha (Alcaligenes eutrophus) CH34.";
RL J. Bacteriol. 182:1399-1409(2000).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor regulates the genes for a membrane-located
CC efflux system that confers resistance to nickel and cobalt.
CC -!- FUNCTION: CnrH alone is able to activate CNR expression, while both
CC CnrY and CrnX are needed for nickel induction of cnrH
CC (PubMed:10671463). Binds DNA in an RNA polymerase-dependent fashion
CC (PubMed:10671464). CnrH may be controlled by a CnrYX transmembrane
CC anti-sigma factor complex which binds CnrH in the absence of Ni(2+). If
CC Ni(2+) appears in the periplasm, it may be bound by CnrR (CnrX); the
CC signal then would be transmitted by CnrY into the cytoplasm and CnrH
CC would be released. {ECO:0000269|PubMed:10671463,
CC ECO:0000269|PubMed:10671464}.
CC -!- INDUCTION: By nickel and cobalt. {ECO:0000269|PubMed:10671463,
CC ECO:0000269|PubMed:10671464}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC {ECO:0000305}.
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DR EMBL; M91650; AAA21967.1; -; Genomic_DNA.
DR EMBL; AJ276513; CAB82450.1; -; Genomic_DNA.
DR EMBL; X90708; CAI30230.1; -; Genomic_DNA.
DR EMBL; CP000355; ABF13066.1; -; Genomic_DNA.
DR RefSeq; WP_011239969.1; NC_007972.2.
DR RefSeq; YP_161708.1; NC_006525.1.
DR PDB; 4CXF; X-ray; 1.75 A; A=1-191.
DR PDBsum; 4CXF; -.
DR AlphaFoldDB; P37978; -.
DR SMR; P37978; -.
DR PRIDE; P37978; -.
DR EnsemblBacteria; ABF13066; ABF13066; Rmet_6207.
DR GeneID; 60825775; -.
DR KEGG; rme:Rmet_6207; -.
DR HOGENOM; CLU_047691_3_0_4; -.
DR OMA; WHALDDF; -.
DR OrthoDB; 1914729at2; -.
DR Proteomes; UP000002429; Plasmid pMOL28.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0006950; P:response to stress; IEA:UniProt.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR039425; RNA_pol_sigma-70-like.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000838; RNA_pol_sigma70_ECF_CS.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR43133; PTHR43133; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF08281; Sigma70_r4_2; 1.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS01063; SIGMA70_ECF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cobalt; DNA-binding; Nickel; Plasmid; Reference proteome;
KW Sigma factor; Transcription; Transcription regulation.
FT CHAIN 1..191
FT /note="RNA polymerase sigma factor CnrH"
FT /id="PRO_0000094009"
FT DNA_BIND 156..175
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT MOTIF 49..62
FT /note="Polymerase core binding"
FT HELIX 6..15
FT /evidence="ECO:0007829|PDB:4CXF"
FT HELIX 19..39
FT /evidence="ECO:0007829|PDB:4CXF"
FT HELIX 44..61
FT /evidence="ECO:0007829|PDB:4CXF"
FT HELIX 62..64
FT /evidence="ECO:0007829|PDB:4CXF"
FT HELIX 71..87
FT /evidence="ECO:0007829|PDB:4CXF"
FT HELIX 126..135
FT /evidence="ECO:0007829|PDB:4CXF"
FT HELIX 140..150
FT /evidence="ECO:0007829|PDB:4CXF"
FT HELIX 156..163
FT /evidence="ECO:0007829|PDB:4CXF"
FT HELIX 167..188
FT /evidence="ECO:0007829|PDB:4CXF"
SQ SEQUENCE 191 AA; 20623 MW; 711DE56029AB4816 CRC64;
MNPEDADRIL AAQAASGNQR AFGQLVARHG VALAQAARSF GIPETDVDDV VQDTFVAAWH
ALDDFDPDRP FRAWLFRIGL NKMRDLYRFR RVRQFLFGAE NLGDLELAGG VANDEPGPEQ
QVAARLELAR VASTLGKLDT GSREVIVLTA IVGMSQPEAA AVLGLSVKAV EGRIGRARAK
LSALLDADSE K
