CNRR_CUPMC
ID CNRR_CUPMC Reviewed; 148 AA.
AC P37975; Q5NUX1; Q7B058;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 2.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Nickel and cobalt resistance protein CnrR;
DE Flags: Precursor;
GN Name=cnrR; Synonyms=cnrX; OrderedLocusNames=Rmet_6206; ORFNames=RMe0087;
OS Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS CH34) (Ralstonia metallidurans).
OG Plasmid pMOL28.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=266264;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8380802; DOI=10.1128/jb.175.3.767-778.1993;
RA Liesegang H., Lemke K., Siddiqui R.A., Schlegel H.-G.;
RT "Characterization of the inducible nickel and cobalt resistance determinant
RT cnr from pMOL28 of Alcaligenes eutrophus CH34.";
RL J. Bacteriol. 175:767-778(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA van der Lelie D., Monchy S., Taghavi S., McCorkle S., Dunn J.,
RA Benotmane M., Vallaeys T., Lapidus A., Mergeay M.;
RT "Sequence and features of the Ralstonia metallidurans CH34 heavy metal
RT plasmids pMOL28 and pMOL30.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT master survivalist in harsh and anthropogenic environments.";
RL PLoS ONE 5:E10433-E10433(2010).
RN [4]
RP CHARACTERIZATION, INDUCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10671463; DOI=10.1128/jb.182.5.1390-1398.2000;
RA Grass G., Grosse C., Nies D.H.;
RT "Regulation of the cnr cobalt and nickel resistance determinant from
RT Ralstonia sp. strain CH34.";
RL J. Bacteriol. 182:1390-1398(2000).
RN [5]
RP SUBCELLULAR LOCATION, INDUCTION, AND SUGGESTION OF NICKEL BINDING.
RX PubMed=10671464; DOI=10.1128/jb.182.5.1399-1409.2000;
RA Tibazarwa C., Wuertz S., Mergeay M., Wyns L., van der Lelie D.;
RT "Regulation of the cnr cobalt and nickel resistance determinant of
RT Ralstonia eutropha (Alcaligenes eutrophus) CH34.";
RL J. Bacteriol. 182:1399-1409(2000).
CC -!- FUNCTION: CnrH alone is able to activate cnr expression, while both
CC CnrY and CrnR (CnrX) are needed for nickel induction of CnrH
CC (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.
CC {ECO:0000269|PubMed:10671463, ECO:0000269|PubMed:10671464}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:10671463,
CC ECO:0000269|PubMed:10671464}.
CC -!- INDUCTION: By nickel and cobalt. {ECO:0000269|PubMed:10671463,
CC ECO:0000269|PubMed:10671464}.
CC -!- SIMILARITY: To A.xylosoxydans NccX. {ECO:0000305}.
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DR EMBL; M91650; AAA21966.1; -; Genomic_DNA.
DR EMBL; AJ276513; CAB82449.1; -; Genomic_DNA.
DR EMBL; X90708; CAI30231.1; -; Genomic_DNA.
DR EMBL; CP000355; ABF13065.1; -; Genomic_DNA.
DR PIR; C47056; C47056.
DR RefSeq; WP_011239970.1; NC_007972.2.
DR RefSeq; YP_161709.1; NC_006525.1.
DR PDB; 2Y39; X-ray; 1.41 A; A=31-148.
DR PDB; 2Y3B; X-ray; 1.55 A; A=31-148.
DR PDB; 2Y3D; X-ray; 2.30 A; A/B=31-148.
DR PDB; 2Y3G; X-ray; 1.91 A; A/B/C/D=31-148.
DR PDB; 2Y3H; X-ray; 1.89 A; A/B/C/D=31-148.
DR PDB; 3EPV; X-ray; 1.74 A; A/B/C/D=40-148.
DR PDB; 3ZG1; X-ray; 1.85 A; A/B/C/D=1-148.
DR PDB; 4WWB; X-ray; 1.11 A; A=31-148.
DR PDB; 4WWD; X-ray; 1.30 A; A=31-148.
DR PDB; 4WWF; X-ray; 1.10 A; A/B=31-148.
DR PDBsum; 2Y39; -.
DR PDBsum; 2Y3B; -.
DR PDBsum; 2Y3D; -.
DR PDBsum; 2Y3G; -.
DR PDBsum; 2Y3H; -.
DR PDBsum; 3EPV; -.
DR PDBsum; 3ZG1; -.
DR PDBsum; 4WWB; -.
DR PDBsum; 4WWD; -.
DR PDBsum; 4WWF; -.
DR AlphaFoldDB; P37975; -.
DR SMR; P37975; -.
DR EnsemblBacteria; ABF13065; ABF13065; Rmet_6206.
DR GeneID; 60825776; -.
DR KEGG; rme:Rmet_6206; -.
DR HOGENOM; CLU_147385_0_0_4; -.
DR OMA; QHLFAMR; -.
DR OrthoDB; 1704773at2; -.
DR EvolutionaryTrace; P37975; -.
DR Proteomes; UP000002429; Plasmid pMOL28.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR InterPro; IPR025961; Metal_resist.
DR Pfam; PF13801; Metal_resist; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cobalt; Coiled coil; Nickel; Periplasm; Plasmid;
KW Reference proteome; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..148
FT /note="Nickel and cobalt resistance protein CnrR"
FT /id="PRO_0000089977"
FT TOPO_DOM 28..148
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT COILED 54..117
FT /evidence="ECO:0000255"
FT CONFLICT 136
FT /note="D -> V (in Ref. 1; AAA21966/CAB82449)"
FT /evidence="ECO:0000305"
FT HELIX 41..48
FT /evidence="ECO:0007829|PDB:4WWF"
FT HELIX 53..90
FT /evidence="ECO:0007829|PDB:4WWF"
FT HELIX 96..126
FT /evidence="ECO:0007829|PDB:4WWF"
FT HELIX 129..131
FT /evidence="ECO:0007829|PDB:4WWF"
FT HELIX 132..144
FT /evidence="ECO:0007829|PDB:4WWF"
SQ SEQUENCE 148 AA; 16633 MW; 1D1B57FE1FF6F36A CRC64;
MMKSRTRRLS LSTLFGALLG VSVAAAWLYY SHRNEAGHGD LHEILHEAVP LDANEREILE
LKEDAFAQRR REIETRLRAA NGKLADAIAK NPAWSPEVEA ATQEVERAAG DLQRATLVHV
FEMRAGLKPE HRPAYDRVLI DALRRGSQ
